Tags

Type your tag names separated by a space and hit enter

Apoptosis in tissues from fatal dengue shock syndrome.
J Clin Virol. 2007 Sep; 40(1):50-4.JC

Abstract

BACKGROUND

Apoptosis, or programmed cell death, has been implicated in dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) pathogenesis.

OBJECTIVES

To determine the in vivo apoptosis contribution to the pathogenesis of fatal DHF/DSS during a Cuban dengue epidemic.

STUDY DESIGN

We detected apoptosis by the TdT-mediated dUTP Nick-End Labeling (TUNEL) technique and dengue virus (DENV) antigens by an immunohistochemical assay in different tissues from six individuals who died of DHF/DSS during the Santiago de Cuba DENV-2 epidemic in 1997.

RESULTS

DENV antigens were immunolocalized mainly in hepatocytes. Apoptotic cells were found in five of the six cases studied. Apoptosis was demonstrated in liver, brain, intestinal and lung tissues. Severe brain hypoxia and ischemia in the studied subjects during DHF/DSS probably might induce apoptosis in cerebral cells. Apoptotic microvascular endothelial cells (ECs) in pulmonary and intestinal tissues, a finding only previously reported in vitro, are likely related to vascular plasma leakage manifested by the individuals.

CONCLUSIONS

Apoptosis was demonstrated in cerebral cells, white blood cells, intestinal and pulmonary microvascular ECs from Cuban fatal cases of DHF/DSS. As far as we know, these findings have not been previously reported in DHF/DSS. Our results indicate there is very likely an in vivo contribution of apoptosis to the pathophysiological mechanisms of DHF/DSS.

Authors+Show Affiliations

Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana, Cuba.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17693133

Citation

Limonta, Daniel, et al. "Apoptosis in Tissues From Fatal Dengue Shock Syndrome." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 40, no. 1, 2007, pp. 50-4.
Limonta D, Capó V, Torres G, et al. Apoptosis in tissues from fatal dengue shock syndrome. J Clin Virol. 2007;40(1):50-4.
Limonta, D., Capó, V., Torres, G., Pérez, A. B., & Guzmán, M. G. (2007). Apoptosis in tissues from fatal dengue shock syndrome. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 40(1), 50-4.
Limonta D, et al. Apoptosis in Tissues From Fatal Dengue Shock Syndrome. J Clin Virol. 2007;40(1):50-4. PubMed PMID: 17693133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis in tissues from fatal dengue shock syndrome. AU - Limonta,Daniel, AU - Capó,Virginia, AU - Torres,Griselda, AU - Pérez,Ana B, AU - Guzmán,María G, Y1 - 2007/08/10/ PY - 2006/12/20/received PY - 2007/04/17/revised PY - 2007/04/25/accepted PY - 2007/8/19/pubmed PY - 2007/12/7/medline PY - 2007/8/19/entrez SP - 50 EP - 4 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 40 IS - 1 N2 - BACKGROUND: Apoptosis, or programmed cell death, has been implicated in dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) pathogenesis. OBJECTIVES: To determine the in vivo apoptosis contribution to the pathogenesis of fatal DHF/DSS during a Cuban dengue epidemic. STUDY DESIGN: We detected apoptosis by the TdT-mediated dUTP Nick-End Labeling (TUNEL) technique and dengue virus (DENV) antigens by an immunohistochemical assay in different tissues from six individuals who died of DHF/DSS during the Santiago de Cuba DENV-2 epidemic in 1997. RESULTS: DENV antigens were immunolocalized mainly in hepatocytes. Apoptotic cells were found in five of the six cases studied. Apoptosis was demonstrated in liver, brain, intestinal and lung tissues. Severe brain hypoxia and ischemia in the studied subjects during DHF/DSS probably might induce apoptosis in cerebral cells. Apoptotic microvascular endothelial cells (ECs) in pulmonary and intestinal tissues, a finding only previously reported in vitro, are likely related to vascular plasma leakage manifested by the individuals. CONCLUSIONS: Apoptosis was demonstrated in cerebral cells, white blood cells, intestinal and pulmonary microvascular ECs from Cuban fatal cases of DHF/DSS. As far as we know, these findings have not been previously reported in DHF/DSS. Our results indicate there is very likely an in vivo contribution of apoptosis to the pathophysiological mechanisms of DHF/DSS. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/17693133/Apoptosis_in_tissues_from_fatal_dengue_shock_syndrome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(07)00159-X DB - PRIME DP - Unbound Medicine ER -