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Effect of intrathecal NG-nitro-L-arginine methyl ester administration on Fos expression in the spinal dorsal horn in rats following sciatic nerve ligation.
Acta Anaesthesiol Taiwan. 2007 Jun; 45(2):65-72.AA

Abstract

BACKGROUND

In the available literature, the pro- or antinociceptive role of nitric oxide (NO) is warmly disputed. As a marker of neuronal activation of the central nervous system, Fos expression has been widely used to assess the change in central neuronal activity evoked by peripheral input. In this study, we examined the effect of intrathecal L-NAME, a non-selective nitric oxide synthase (NOS) inhibitor, on nociceptive behavior and spinal Fos expression in rats following chronic constriction injury (CCI) of sciatic nerve, a model of neuropathic pain similar to that observed in clinical setting.

METHODS

Eighty adult male SD rats showing no neurological deficiency a week after intrathecal catheterization were used in this study. L-NAME 250 microg of 10 microl (an equivalent or 0.9% saline) was injected intrathecally 15 min prior to CCI or sham operation. In addition to examination of thermal hyperalgesia by paw withdrawal latency (PWL), measurement of Fos protein staining neurons in the lumbar spinal cord using an immunohistochemistry technique were made at 1, 3, 7 and 14 days after operation.

RESULTS

As compared with untreated animals, both CCI and sham operations evoked an early and long-term Fos expression, whereas a significant decrease in PWL was demonstrated only in rats receiving CCI. On days 3, 7 and 14 after CCI, the number of FLI neurons in the spinal dorsal horn ipsilateral to the injury decreased by 54%, 57% and 43%, respectively, in CCI-L-NAME group when compared with CCI-saline group, corresponding to the significant attenuation of thermal hyperalgesia. However, intrathecal L-NAME preadministration had no effect on the spinal Fos expression evoked by sham operation.

CONCLUSIONS

Spinal Fos expression could be induced by different mechanisms, and it should not regarded as a reliable marker of pain sensation disorders. NO plays an important role in the development of nociception and spinal Fos expression through central sensitization mediated by peripheral nerve injury.

Authors+Show Affiliations

Li P
Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Xue FS
No affiliation info available
Li CW
No affiliation info available
Liu KP
No affiliation info available
Liu Y
No affiliation info available
Xu YC
No affiliation info available
Yang QY
No affiliation info available

MeSH

AnimalsDogsHyperalgesiaImmunohistochemistryInjections, SpinalNG-Nitroarginine Methyl EsterNitric OxidePosterior Horn CellsProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleyReceptors, N-Methyl-D-AspartateSciatic Neuropathy

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17694681

Citation

Li, Ping, et al. "Effect of Intrathecal NG-nitro-L-arginine Methyl Ester Administration On Fos Expression in the Spinal Dorsal Horn in Rats Following Sciatic Nerve Ligation." Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists, vol. 45, no. 2, 2007, pp. 65-72.
Li P, Xue FS, Li CW, et al. Effect of intrathecal NG-nitro-L-arginine methyl ester administration on Fos expression in the spinal dorsal horn in rats following sciatic nerve ligation. Acta Anaesthesiol Taiwan. 2007;45(2):65-72.
Li, P., Xue, F. S., Li, C. W., Liu, K. P., Liu, Y., Xu, Y. C., & Yang, Q. Y. (2007). Effect of intrathecal NG-nitro-L-arginine methyl ester administration on Fos expression in the spinal dorsal horn in rats following sciatic nerve ligation. Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists, 45(2), 65-72.
Li P, et al. Effect of Intrathecal NG-nitro-L-arginine Methyl Ester Administration On Fos Expression in the Spinal Dorsal Horn in Rats Following Sciatic Nerve Ligation. Acta Anaesthesiol Taiwan. 2007;45(2):65-72. PubMed PMID: 17694681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of intrathecal NG-nitro-L-arginine methyl ester administration on Fos expression in the spinal dorsal horn in rats following sciatic nerve ligation. AU - Li,Ping, AU - Xue,Fu-Shan, AU - Li,Cheng-Wen, AU - Liu,Kun-Peng, AU - Liu,Yi, AU - Xu,Ya-Chao, AU - Yang,Quan-Yong, PY - 2007/8/19/pubmed PY - 2007/8/31/medline PY - 2007/8/19/entrez SP - 65 EP - 72 JF - Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists JO - Acta Anaesthesiol Taiwan VL - 45 IS - 2 N2 - BACKGROUND: In the available literature, the pro- or antinociceptive role of nitric oxide (NO) is warmly disputed. As a marker of neuronal activation of the central nervous system, Fos expression has been widely used to assess the change in central neuronal activity evoked by peripheral input. In this study, we examined the effect of intrathecal L-NAME, a non-selective nitric oxide synthase (NOS) inhibitor, on nociceptive behavior and spinal Fos expression in rats following chronic constriction injury (CCI) of sciatic nerve, a model of neuropathic pain similar to that observed in clinical setting. METHODS: Eighty adult male SD rats showing no neurological deficiency a week after intrathecal catheterization were used in this study. L-NAME 250 microg of 10 microl (an equivalent or 0.9% saline) was injected intrathecally 15 min prior to CCI or sham operation. In addition to examination of thermal hyperalgesia by paw withdrawal latency (PWL), measurement of Fos protein staining neurons in the lumbar spinal cord using an immunohistochemistry technique were made at 1, 3, 7 and 14 days after operation. RESULTS: As compared with untreated animals, both CCI and sham operations evoked an early and long-term Fos expression, whereas a significant decrease in PWL was demonstrated only in rats receiving CCI. On days 3, 7 and 14 after CCI, the number of FLI neurons in the spinal dorsal horn ipsilateral to the injury decreased by 54%, 57% and 43%, respectively, in CCI-L-NAME group when compared with CCI-saline group, corresponding to the significant attenuation of thermal hyperalgesia. However, intrathecal L-NAME preadministration had no effect on the spinal Fos expression evoked by sham operation. CONCLUSIONS: Spinal Fos expression could be induced by different mechanisms, and it should not regarded as a reliable marker of pain sensation disorders. NO plays an important role in the development of nociception and spinal Fos expression through central sensitization mediated by peripheral nerve injury. SN - 1875-4597 UR - https://www.unboundmedicine.com/medline/citation/17694681/Effect_of_intrathecal_NG_nitro_L_arginine_methyl_ester_administration_on_Fos_expression_in_the_spinal_dorsal_horn_in_rats_following_sciatic_nerve_ligation_ L2 - https://antibodies.cancer.gov/detail/CPTC-FOS-4 DB - PRIME DP - Unbound Medicine ER -