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Elevated matrilysin levels in bronchoalveolar lavage fluid do not distinguish idiopathic pulmonary fibrosis from other interstitial lung diseases.
APMIS. 2007 Aug; 115(8):969-75.A

Abstract

Microarray studies have shown that matrilysin or matrix metalloproteinase (MMP)-7 is highly upregulated in the lungs of patients with idiopathic pulmonary fibrosis (IPF), but MMP-7 protein expression has not been systematically compared between IPF and other interstitial lung diseases. MMP-7 levels in bronchoalveolar lavage fluid (BALF) were compared to corresponding samples from nonspecific interstitial pneumonia (NSIP), sarcoidosis, and healthy controls. MMP-7 levels in the BALF were determined by ELISA and localization of MMP-7 in the lung tissue by immunohistochemistry. MMP-7 was similarly elevated in the BALF of all these disorders compared to healthy controls (p=0.007). Even control subjects with prolonged cough displayed a tendency towards elevated MMP-7 expression. There was a negative correlation between BALF MMP-7 levels and forced expiratory vital capacity (r=-0.348, p=0.02, n=42). In IPF lung, MMP-7 immunoreactivity appeared predominantly in the fibrotic parenchyma and arterial wall. In sarcoidosis and NSIP, prominent MMP-7 immunoreactivity was found in areas of inflammation. These results demonstrate that elevated BALF MMP-7 is not restricted to IPF alone but is also observed in other interstitial lung diseases and cannot be used as a differential diagnostic marker for IPF.

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Authors+Show Affiliations

Vuorinen K
Department of Medicine and Division of Pulmonary Medicine, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
Myllärniemi M
No affiliation info available
Lammi L
No affiliation info available
Piirilä P
No affiliation info available
Rytilä P
No affiliation info available
Salmenkivi K
No affiliation info available
Kinnula VL
No affiliation info available

MeSH

AdultAgedBronchoalveolar Lavage FluidDiagnosis, DifferentialFemaleHumansImmunohistochemistryLungLung Diseases, InterstitialMaleMatrix Metalloproteinase 7Middle AgedPulmonary Fibrosis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17696954

Citation

Vuorinen, Kirsi, et al. "Elevated Matrilysin Levels in Bronchoalveolar Lavage Fluid Do Not Distinguish Idiopathic Pulmonary Fibrosis From Other Interstitial Lung Diseases." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 115, no. 8, 2007, pp. 969-75.
Vuorinen K, Myllärniemi M, Lammi L, et al. Elevated matrilysin levels in bronchoalveolar lavage fluid do not distinguish idiopathic pulmonary fibrosis from other interstitial lung diseases. APMIS. 2007;115(8):969-75.
Vuorinen, K., Myllärniemi, M., Lammi, L., Piirilä, P., Rytilä, P., Salmenkivi, K., & Kinnula, V. L. (2007). Elevated matrilysin levels in bronchoalveolar lavage fluid do not distinguish idiopathic pulmonary fibrosis from other interstitial lung diseases. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 115(8), 969-75.
Vuorinen K, et al. Elevated Matrilysin Levels in Bronchoalveolar Lavage Fluid Do Not Distinguish Idiopathic Pulmonary Fibrosis From Other Interstitial Lung Diseases. APMIS. 2007;115(8):969-75. PubMed PMID: 17696954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated matrilysin levels in bronchoalveolar lavage fluid do not distinguish idiopathic pulmonary fibrosis from other interstitial lung diseases. AU - Vuorinen,Kirsi, AU - Myllärniemi,Marjukka, AU - Lammi,Lauri, AU - Piirilä,Päivi, AU - Rytilä,Paula, AU - Salmenkivi,Kaisa, AU - Kinnula,Vuokko L, PY - 2007/8/19/pubmed PY - 2007/9/21/medline PY - 2007/8/19/entrez SP - 969 EP - 75 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 115 IS - 8 N2 - Microarray studies have shown that matrilysin or matrix metalloproteinase (MMP)-7 is highly upregulated in the lungs of patients with idiopathic pulmonary fibrosis (IPF), but MMP-7 protein expression has not been systematically compared between IPF and other interstitial lung diseases. MMP-7 levels in bronchoalveolar lavage fluid (BALF) were compared to corresponding samples from nonspecific interstitial pneumonia (NSIP), sarcoidosis, and healthy controls. MMP-7 levels in the BALF were determined by ELISA and localization of MMP-7 in the lung tissue by immunohistochemistry. MMP-7 was similarly elevated in the BALF of all these disorders compared to healthy controls (p=0.007). Even control subjects with prolonged cough displayed a tendency towards elevated MMP-7 expression. There was a negative correlation between BALF MMP-7 levels and forced expiratory vital capacity (r=-0.348, p=0.02, n=42). In IPF lung, MMP-7 immunoreactivity appeared predominantly in the fibrotic parenchyma and arterial wall. In sarcoidosis and NSIP, prominent MMP-7 immunoreactivity was found in areas of inflammation. These results demonstrate that elevated BALF MMP-7 is not restricted to IPF alone but is also observed in other interstitial lung diseases and cannot be used as a differential diagnostic marker for IPF. SN - 0903-4641 UR - https://www.unboundmedicine.com/medline/citation/17696954/Elevated_matrilysin_levels_in_bronchoalveolar_lavage_fluid_do_not_distinguish_idiopathic_pulmonary_fibrosis_from_other_interstitial_lung_diseases_ L2 - https://doi.org/10.1111/j.1600-0463.2007.apm_697.x DB - PRIME DP - Unbound Medicine ER -