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Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae.
Vet Parasitol. 2007 Oct 21; 149(1-2):117-25.VP

Abstract

This study identified and characterized hydrolytic enzymes in salivary gland products of Oestrus ovis larvae. Third instars were collected from the heads of slaughtered goats. Salivary glands were extracted, their products obtained by centrifugation and the enzymatic profile determined. Optimum pH, temperature of maximum proteolytic activity, thermal stability, and resistance of salivary gland products were determined on collagen and subclasses of proteases were identified using protease inhibitors. Zymograms were used to determine the molecular weight of proteases. Antigenic protein bands were revealed by immunoblotting using sera obtained from experimentally infested goats. Seven positive enzymatic activities were detected in salivary gland products: acid phosphatase, naphthol-AS-BI-phosphohydrolase, esterase (C4), esterase lipase (C8), leucine arylamidase, alpha-glucosidase and N-acetyl-beta-glucosaminidase. Optimum pH for proteolytic activity was 8.0; proteolytic activity increased with temperature (10-50 degrees C) then drastically decreased at 60 degrees C. Proteases in O. ovis salivary gland products belong to the serine subclass. In Zymograms, bands of proteolytic activity were detected in the 20-63 kDa range; the immunoblot showed three antigenic bands, one of them related to a protease band (63 kDa). Serine proteases in O. ovis salivary gland products are most likely involved in larval nutrition and host immuno-modulation.

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Authors+Show Affiliations

Angulo-Valadez CE
Centro de Investigaciones Biológicas del Noroeste, Mar Bermejo 195, Col. Playa de Santa Rita, La Paz, B.C.S. 23090, Mexico.
Cepeda-Palacios R
No affiliation info available
Ascencio F
No affiliation info available
Jacquiet P
No affiliation info available
Dorchies P
No affiliation info available
Romero MJ
No affiliation info available
Khelifa RM
No affiliation info available

MeSH

AnimalsAntigensDipteraGoat DiseasesGoatsHydrogen-Ion ConcentrationImmunoblottingLarvaMyiasisPeptide HydrolasesProtease InhibitorsSalivary GlandsSalivary Proteins and PeptidesTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17697751

Citation

Angulo-Valadez, C E., et al. "Proteolytic Activity in Salivary Gland Products of Sheep Bot Fly (Oestrus Ovis) Larvae." Veterinary Parasitology, vol. 149, no. 1-2, 2007, pp. 117-25.
Angulo-Valadez CE, Cepeda-Palacios R, Ascencio F, et al. Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae. Vet Parasitol. 2007;149(1-2):117-25.
Angulo-Valadez, C. E., Cepeda-Palacios, R., Ascencio, F., Jacquiet, P., Dorchies, P., Romero, M. J., & Khelifa, R. M. (2007). Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae. Veterinary Parasitology, 149(1-2), 117-25.
Angulo-Valadez CE, et al. Proteolytic Activity in Salivary Gland Products of Sheep Bot Fly (Oestrus Ovis) Larvae. Vet Parasitol. 2007 Oct 21;149(1-2):117-25. PubMed PMID: 17697751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae. AU - Angulo-Valadez,C E, AU - Cepeda-Palacios,R, AU - Ascencio,F, AU - Jacquiet,P, AU - Dorchies,P, AU - Romero,M J, AU - Khelifa,R M, Y1 - 2007/08/13/ PY - 2007/02/14/received PY - 2007/06/27/revised PY - 2007/06/27/accepted PY - 2007/8/19/pubmed PY - 2008/11/14/medline PY - 2007/8/19/entrez SP - 117 EP - 25 JF - Veterinary parasitology JO - Vet Parasitol VL - 149 IS - 1-2 N2 - This study identified and characterized hydrolytic enzymes in salivary gland products of Oestrus ovis larvae. Third instars were collected from the heads of slaughtered goats. Salivary glands were extracted, their products obtained by centrifugation and the enzymatic profile determined. Optimum pH, temperature of maximum proteolytic activity, thermal stability, and resistance of salivary gland products were determined on collagen and subclasses of proteases were identified using protease inhibitors. Zymograms were used to determine the molecular weight of proteases. Antigenic protein bands were revealed by immunoblotting using sera obtained from experimentally infested goats. Seven positive enzymatic activities were detected in salivary gland products: acid phosphatase, naphthol-AS-BI-phosphohydrolase, esterase (C4), esterase lipase (C8), leucine arylamidase, alpha-glucosidase and N-acetyl-beta-glucosaminidase. Optimum pH for proteolytic activity was 8.0; proteolytic activity increased with temperature (10-50 degrees C) then drastically decreased at 60 degrees C. Proteases in O. ovis salivary gland products belong to the serine subclass. In Zymograms, bands of proteolytic activity were detected in the 20-63 kDa range; the immunoblot showed three antigenic bands, one of them related to a protease band (63 kDa). Serine proteases in O. ovis salivary gland products are most likely involved in larval nutrition and host immuno-modulation. SN - 0304-4017 UR - https://www.unboundmedicine.com/medline/citation/17697751/Proteolytic_activity_in_salivary_gland_products_of_sheep_bot_fly__Oestrus_ovis__larvae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(07)00333-0 DB - PRIME DP - Unbound Medicine ER -