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Effects of trichlorfon on malondialdehyde and antioxidant system in human erythrocytes.

Abstract

Organophosphorus insecticides may induce oxidative stress leading to generation of free radicals and alteration in antioxidant system. The aim of this study was to examine the potency of trichlorfon, an organophosphate insecticide, to induce oxidative stress response in human erythrocytes in vitro. For this purpose trichlorfon solutions in different concentrations and erythrocyte solutions were incubated at 37 degrees C for 60 min. At the end of the incubation time, malondialdehyde (MDA), an end product of lipid peroxidation, total glutathione, reduced glutathione (GSH) levels, activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzymes were determined by spectrophotometric methods. Trichlorfon increased MDA formation depended on the concentration. On the other hand, decreases in the GSH-Px activity, GSH levels and increases in the total glutathione levels, SOD and CAT activities were seen in the studied concentrations. The present findings indicate that the in vitro toxicity of trichlorfon may be associated with oxidative stress.

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  • Authors+Show Affiliations

    ,

    Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey.

    ,

    Source

    MeSH

    Antioxidants
    Catalase
    Dose-Response Relationship, Drug
    Erythrocytes
    Glutathione
    Glutathione Peroxidase
    Humans
    Insecticides
    Lipid Peroxidation
    Malondialdehyde
    Oxidative Stress
    Superoxide Dismutase
    Trichlorfon

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17697763

    Citation

    Karademir Catalgol, Betul, et al. "Effects of Trichlorfon On Malondialdehyde and Antioxidant System in Human Erythrocytes." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 21, no. 8, 2007, pp. 1538-44.
    Karademir Catalgol B, Ozden S, Alpertunga B. Effects of trichlorfon on malondialdehyde and antioxidant system in human erythrocytes. Toxicol In Vitro. 2007;21(8):1538-44.
    Karademir Catalgol, B., Ozden, S., & Alpertunga, B. (2007). Effects of trichlorfon on malondialdehyde and antioxidant system in human erythrocytes. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 21(8), pp. 1538-44.
    Karademir Catalgol B, Ozden S, Alpertunga B. Effects of Trichlorfon On Malondialdehyde and Antioxidant System in Human Erythrocytes. Toxicol In Vitro. 2007;21(8):1538-44. PubMed PMID: 17697763.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of trichlorfon on malondialdehyde and antioxidant system in human erythrocytes. AU - Karademir Catalgol,Betul, AU - Ozden,Sibel, AU - Alpertunga,Buket, Y1 - 2007/06/27/ PY - 2007/01/19/received PY - 2007/05/10/revised PY - 2007/06/05/accepted PY - 2007/8/19/pubmed PY - 2008/2/19/medline PY - 2007/8/19/entrez SP - 1538 EP - 44 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 21 IS - 8 N2 - Organophosphorus insecticides may induce oxidative stress leading to generation of free radicals and alteration in antioxidant system. The aim of this study was to examine the potency of trichlorfon, an organophosphate insecticide, to induce oxidative stress response in human erythrocytes in vitro. For this purpose trichlorfon solutions in different concentrations and erythrocyte solutions were incubated at 37 degrees C for 60 min. At the end of the incubation time, malondialdehyde (MDA), an end product of lipid peroxidation, total glutathione, reduced glutathione (GSH) levels, activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzymes were determined by spectrophotometric methods. Trichlorfon increased MDA formation depended on the concentration. On the other hand, decreases in the GSH-Px activity, GSH levels and increases in the total glutathione levels, SOD and CAT activities were seen in the studied concentrations. The present findings indicate that the in vitro toxicity of trichlorfon may be associated with oxidative stress. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/17697763/Effects_of_trichlorfon_on_malondialdehyde_and_antioxidant_system_in_human_erythrocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(07)00175-0 DB - PRIME DP - Unbound Medicine ER -