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Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats.

Abstract

The protective efficacy of diallyl tetrasulfide (DTS) from garlic on liver injury induced by cadmium (Cd) was investigated. In this study, Cd (3 mg/kg body weight) was administered subcutaneously for 3 weeks to induce toxicity. DTS was administered orally (10, 20 and 40 mg/kg body weight) for 3 weeks with subcutaneous (sc) injection of Cd. Cd-induced liver damage was evidenced from increased activities of serum hepatic enzymes, namely aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate dehydrogenase, with significant elevation of lipid peroxidation indices (thiobarbituric acid reactive substances and hydroperoxides) and protein carbonyl groups in the liver. Rats subjected to Cd toxicity also showed a decline in the levels of total thiols, reduced glutathione (GSH), vitamin C and vitamin E, accompanied by an increased accumulation of Cd, and significantly decreased activities of superoxide dismutase, catalase (CAT), glutathione peroxidase, glutathione-S-transferase (GST), glutathione reductase, and glucose-6-phosphate dehydrogenase in the liver. Administration of DTS at 40 mg/kg body weight significantly normalised the activities of hepatic marker enzymes, compared to other doses of DTS (10 and 20 mg/kg body weight). In addition, DTS (40 mg/kg body weight) significantly reduced the accumulation of Cd and the level of lipid peroxidation, and restored the level of antioxidant defense in the liver. Histological studies also showed that administration of DTS to Cd-treated rats resulted in a marked improvement of hepatocytes morphology with mild portal inflammation. Our results suggest that DTS might play a vital role in protecting Cd-induced oxidative damage in the liver.

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  • Authors+Show Affiliations

    ,

    Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.

    Source

    Human & experimental toxicology 26:6 2007 Jun pg 527-34

    MeSH

    Administration, Oral
    Allyl Compounds
    Animals
    Antioxidants
    Cadmium Chloride
    Catalase
    Chemical and Drug Induced Liver Injury
    Dose-Response Relationship, Drug
    Glucosephosphate Dehydrogenase
    Glutathione Peroxidase
    Glutathione Reductase
    Injections, Subcutaneous
    Intubation, Gastrointestinal
    Lipid Peroxidation
    Liver
    Liver Diseases
    Male
    Oxidative Stress
    Protein Carbonylation
    Rats
    Rats, Wistar
    Sulfides
    Superoxide Dismutase
    Thiobarbituric Acid Reactive Substances

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17698948

    Citation

    Murugavel, P, and L Pari. "Effects of Diallyl Tetrasulfide On Cadmium-induced Oxidative Damage in the Liver of Rats." Human & Experimental Toxicology, vol. 26, no. 6, 2007, pp. 527-34.
    Murugavel P, Pari L. Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats. Hum Exp Toxicol. 2007;26(6):527-34.
    Murugavel, P., & Pari, L. (2007). Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats. Human & Experimental Toxicology, 26(6), pp. 527-34.
    Murugavel P, Pari L. Effects of Diallyl Tetrasulfide On Cadmium-induced Oxidative Damage in the Liver of Rats. Hum Exp Toxicol. 2007;26(6):527-34. PubMed PMID: 17698948.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats. AU - Murugavel,P, AU - Pari,L, PY - 2007/8/21/pubmed PY - 2007/10/17/medline PY - 2007/8/21/entrez SP - 527 EP - 34 JF - Human & experimental toxicology JO - Hum Exp Toxicol VL - 26 IS - 6 N2 - The protective efficacy of diallyl tetrasulfide (DTS) from garlic on liver injury induced by cadmium (Cd) was investigated. In this study, Cd (3 mg/kg body weight) was administered subcutaneously for 3 weeks to induce toxicity. DTS was administered orally (10, 20 and 40 mg/kg body weight) for 3 weeks with subcutaneous (sc) injection of Cd. Cd-induced liver damage was evidenced from increased activities of serum hepatic enzymes, namely aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate dehydrogenase, with significant elevation of lipid peroxidation indices (thiobarbituric acid reactive substances and hydroperoxides) and protein carbonyl groups in the liver. Rats subjected to Cd toxicity also showed a decline in the levels of total thiols, reduced glutathione (GSH), vitamin C and vitamin E, accompanied by an increased accumulation of Cd, and significantly decreased activities of superoxide dismutase, catalase (CAT), glutathione peroxidase, glutathione-S-transferase (GST), glutathione reductase, and glucose-6-phosphate dehydrogenase in the liver. Administration of DTS at 40 mg/kg body weight significantly normalised the activities of hepatic marker enzymes, compared to other doses of DTS (10 and 20 mg/kg body weight). In addition, DTS (40 mg/kg body weight) significantly reduced the accumulation of Cd and the level of lipid peroxidation, and restored the level of antioxidant defense in the liver. Histological studies also showed that administration of DTS to Cd-treated rats resulted in a marked improvement of hepatocytes morphology with mild portal inflammation. Our results suggest that DTS might play a vital role in protecting Cd-induced oxidative damage in the liver. SN - 0960-3271 UR - https://www.unboundmedicine.com/medline/citation/17698948/Effects_of_diallyl_tetrasulfide_on_cadmium_induced_oxidative_damage_in_the_liver_of_rats_ L2 - http://journals.sagepub.com/doi/full/10.1177/0960327107073810?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -