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[Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints].
Yao Xue Xue Bao. 2007 May; 42(5):566-70.YX

Abstract

To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints. The samples were extracted using ASE for 10 min under 100 degrees C and 9.65 MPa, and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively. Based on this method, the HPLC fingerprints of Radix gentianae were established. Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value, three compounds in water phase were identified as gentiopicroside, asafetida acid and loganic acid. There is no report of the compounds in chloroform phase. The content of gentiopicroside and loganic acid in samples of different groups were determined, separately. The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint. The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90, however, the chloroform phase fingerprint congruence coefficients were within 0.62 -0.99. This method can be used for determination of potent component in Radix gentianae and its quality control. Radix gentianae from different producing areas have the largest diversities, and the diversities embodied in the content of chloroform phase compounds.

Authors+Show Affiliations

Li WL
Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of State Oceanic Administration People's Republic of China, Qingdao 266061, China.
Chen JH
No affiliation info available
Yin YF
No affiliation info available
Wu FQ
No affiliation info available
Yang BJ
No affiliation info available
Yang HH
No affiliation info available
Wang XR
No affiliation info available

MeSH

Chromatography, High Pressure LiquidEcosystemGentianaGlucosidesIridoid GlucosidesIridoidsPlant RootsPlants, MedicinalReproducibility of ResultsSensitivity and SpecificitySpectrometry, Mass, Electrospray Ionization

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17703785

Citation

Li, Wen-Long, et al. "[Content of Gentiopicroside and Loganic Acid in Radix Gentianae and Their Fingerprints]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 42, no. 5, 2007, pp. 566-70.
Li WL, Chen JH, Yin YF, et al. [Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints]. Yao Xue Xue Bao. 2007;42(5):566-70.
Li, W. L., Chen, J. H., Yin, Y. F., Wu, F. Q., Yang, B. J., Yang, H. H., & Wang, X. R. (2007). [Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 42(5), 566-70.
Li WL, et al. [Content of Gentiopicroside and Loganic Acid in Radix Gentianae and Their Fingerprints]. Yao Xue Xue Bao. 2007;42(5):566-70. PubMed PMID: 17703785.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints]. AU - Li,Wen-Long, AU - Chen,Jun-Hui, AU - Yin,Yue-Fen, AU - Wu,Feng-Qi, AU - Yang,Bai-Juan, AU - Yang,Huang-Hao, AU - Wang,Xiao-Ru, PY - 2007/8/21/pubmed PY - 2009/7/17/medline PY - 2007/8/21/entrez SP - 566 EP - 70 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 42 IS - 5 N2 - To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints. The samples were extracted using ASE for 10 min under 100 degrees C and 9.65 MPa, and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively. Based on this method, the HPLC fingerprints of Radix gentianae were established. Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value, three compounds in water phase were identified as gentiopicroside, asafetida acid and loganic acid. There is no report of the compounds in chloroform phase. The content of gentiopicroside and loganic acid in samples of different groups were determined, separately. The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint. The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90, however, the chloroform phase fingerprint congruence coefficients were within 0.62 -0.99. This method can be used for determination of potent component in Radix gentianae and its quality control. Radix gentianae from different producing areas have the largest diversities, and the diversities embodied in the content of chloroform phase compounds. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/17703785/[Content_of_gentiopicroside_and_loganic_acid_in_Radix_gentianae_and_their_fingerprints]_ DB - PRIME DP - Unbound Medicine ER -