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Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction.
Plant Physiol. 2007 Oct; 145(2):450-64.PP

Abstract

Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabidopsis thaliana), encodes a protein possessing in vitro adenylation activity on both indole-3-acetic acid (IAA) and SA. Here, we show that GH3.5 acts as a bifunctional modulator in both SA and auxin signaling during pathogen infection. Overexpression of the GH3.5 gene in an activation-tagged mutant gh3.5-1D led to elevated accumulation of SA and increased expression of PR-1 in local and systemic tissues in response to avirulent pathogens. In contrast, two T-DNA insertional mutations of GH3.5 partially compromised the systemic acquired resistance associated with diminished PR-1 expression in systemic tissues. The gh3.5-1D mutant also accumulated high levels of free IAA after pathogen infection and impaired different resistance-gene-mediated resistance, which was also observed in the GH3.6 activation-tagged mutant dfl1-D that impacted the auxin pathway, indicating an important role of GH3.5/GH3.6 in disease susceptibility. Furthermore, microarray analysis showed that the SA and auxin pathways were simultaneously augmented in gh3.5-1D after infection with an avirulent pathogen. The SA pathway was amplified by GH3.5 through inducing SA-responsive genes and basal defense components, whereas the auxin pathway was derepressed through up-regulating IAA biosynthesis and down-regulating auxin repressor genes. Taken together, our data reveal novel regulatory functions of GH3.5 in the plant-pathogen interaction.

Authors+Show Affiliations

National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17704230

Citation

Zhang, Zhongqin, et al. "Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin Signaling During Arabidopsis-Pseudomonas Syringae Interaction." Plant Physiology, vol. 145, no. 2, 2007, pp. 450-64.
Zhang Z, Li Q, Li Z, et al. Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction. Plant Physiol. 2007;145(2):450-64.
Zhang, Z., Li, Q., Li, Z., Staswick, P. E., Wang, M., Zhu, Y., & He, Z. (2007). Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction. Plant Physiology, 145(2), 450-64.
Zhang Z, et al. Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin Signaling During Arabidopsis-Pseudomonas Syringae Interaction. Plant Physiol. 2007;145(2):450-64. PubMed PMID: 17704230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction. AU - Zhang,Zhongqin, AU - Li,Qun, AU - Li,Zhimiao, AU - Staswick,Paul E, AU - Wang,Muyang, AU - Zhu,Ying, AU - He,Zuhua, Y1 - 2007/08/17/ PY - 2007/8/21/pubmed PY - 2008/1/4/medline PY - 2007/8/21/entrez SP - 450 EP - 64 JF - Plant physiology JO - Plant Physiol VL - 145 IS - 2 N2 - Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabidopsis thaliana), encodes a protein possessing in vitro adenylation activity on both indole-3-acetic acid (IAA) and SA. Here, we show that GH3.5 acts as a bifunctional modulator in both SA and auxin signaling during pathogen infection. Overexpression of the GH3.5 gene in an activation-tagged mutant gh3.5-1D led to elevated accumulation of SA and increased expression of PR-1 in local and systemic tissues in response to avirulent pathogens. In contrast, two T-DNA insertional mutations of GH3.5 partially compromised the systemic acquired resistance associated with diminished PR-1 expression in systemic tissues. The gh3.5-1D mutant also accumulated high levels of free IAA after pathogen infection and impaired different resistance-gene-mediated resistance, which was also observed in the GH3.6 activation-tagged mutant dfl1-D that impacted the auxin pathway, indicating an important role of GH3.5/GH3.6 in disease susceptibility. Furthermore, microarray analysis showed that the SA and auxin pathways were simultaneously augmented in gh3.5-1D after infection with an avirulent pathogen. The SA pathway was amplified by GH3.5 through inducing SA-responsive genes and basal defense components, whereas the auxin pathway was derepressed through up-regulating IAA biosynthesis and down-regulating auxin repressor genes. Taken together, our data reveal novel regulatory functions of GH3.5 in the plant-pathogen interaction. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/17704230/Dual_regulation_role_of_GH3_5_in_salicylic_acid_and_auxin_signaling_during_Arabidopsis_Pseudomonas_syringae_interaction_ L2 - http://www.plantphysiol.org/lookup/pmidlookup?view=long&pmid=17704230 DB - PRIME DP - Unbound Medicine ER -