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The effect of glypican-1 glycosaminoglycan chains on turkey myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness.
Poult Sci. 2007 Sep; 86(9):2020-8.PS

Abstract

The glypicans are a family of cell-surface heparan sulfate proteoglycans consisting of a core protein covalently attached with glycosaminoglycans (GAG). Only glypican-1 is expressed in skeletal muscle and increases in expression during myoblast differentiation. Previous studies have suggested that glypican-1 influences fibroblast growth factor 2 (FGF2) signaling pathway by its heparan sulfate chains. Fibroblast growth factor 2 is a potent stimulator of muscle cell proliferation and an intense inhibitor of differentiation. To investigate the functional contribution of each GAG chain attachment site, a turkey glypican-1 full length cDNA (1,650 bp, Gen-Bank accession number AY551002) was cloned into the pCMS-EGFP vector and mutated at 2 or all 3 potential GAG attachment sites at Ser(483), Ser(485), and Ser(487) to obtain 1-chain and no-chain mutants, respectively. The unmutated glypican-1, 1-chain, and no-chain mutants, and the pCMS-EGFP vector without an insert were transfected into turkey myogenic satellite cells. The transfected cell cultures were assayed for cell proliferation, differentiation, and FGF2 responsiveness. The overexpression of glypican-1 increased FGF2 responsiveness during proliferation compared with the 1-chain, no-chain mutants, and the pCMS-EGFP vector without an insert, but there was no significant interaction between FGF2 and glypican-1. The overexpression of glypican-1 also increased differentiation but did not affect proliferation when compared with the 1-chain, no-chain mutants, and the pCMS-EGFP vector without an insert. To support the overexpression data, glypican-1 expression was reduced using a small interfering RNA against turkey glypican-1. Inhibition of glypican-1 expression decreased myogenic satellite cell proliferation, differentiation, and FGF2 responsiveness during proliferation. These data indicate that glypican-1 function requires the GAG chain attachment sites for myogenic satellite cell FGF2 responsiveness during proliferation and to affect the process of differentiation.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster Ohio 44691, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17704393

Citation

Zhang, X, et al. "The Effect of Glypican-1 Glycosaminoglycan Chains On Turkey Myogenic Satellite Cell Proliferation, Differentiation, and Fibroblast Growth Factor 2 Responsiveness." Poultry Science, vol. 86, no. 9, 2007, pp. 2020-8.
Zhang X, Liu C, Nestor KE, et al. The effect of glypican-1 glycosaminoglycan chains on turkey myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. Poult Sci. 2007;86(9):2020-8.
Zhang, X., Liu, C., Nestor, K. E., McFarland, D. C., & Velleman, S. G. (2007). The effect of glypican-1 glycosaminoglycan chains on turkey myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. Poultry Science, 86(9), 2020-8.
Zhang X, et al. The Effect of Glypican-1 Glycosaminoglycan Chains On Turkey Myogenic Satellite Cell Proliferation, Differentiation, and Fibroblast Growth Factor 2 Responsiveness. Poult Sci. 2007;86(9):2020-8. PubMed PMID: 17704393.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of glypican-1 glycosaminoglycan chains on turkey myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. AU - Zhang,X, AU - Liu,C, AU - Nestor,K E, AU - McFarland,D C, AU - Velleman,S G, PY - 2007/8/21/pubmed PY - 2007/12/21/medline PY - 2007/8/21/entrez SP - 2020 EP - 8 JF - Poultry science JO - Poult Sci VL - 86 IS - 9 N2 - The glypicans are a family of cell-surface heparan sulfate proteoglycans consisting of a core protein covalently attached with glycosaminoglycans (GAG). Only glypican-1 is expressed in skeletal muscle and increases in expression during myoblast differentiation. Previous studies have suggested that glypican-1 influences fibroblast growth factor 2 (FGF2) signaling pathway by its heparan sulfate chains. Fibroblast growth factor 2 is a potent stimulator of muscle cell proliferation and an intense inhibitor of differentiation. To investigate the functional contribution of each GAG chain attachment site, a turkey glypican-1 full length cDNA (1,650 bp, Gen-Bank accession number AY551002) was cloned into the pCMS-EGFP vector and mutated at 2 or all 3 potential GAG attachment sites at Ser(483), Ser(485), and Ser(487) to obtain 1-chain and no-chain mutants, respectively. The unmutated glypican-1, 1-chain, and no-chain mutants, and the pCMS-EGFP vector without an insert were transfected into turkey myogenic satellite cells. The transfected cell cultures were assayed for cell proliferation, differentiation, and FGF2 responsiveness. The overexpression of glypican-1 increased FGF2 responsiveness during proliferation compared with the 1-chain, no-chain mutants, and the pCMS-EGFP vector without an insert, but there was no significant interaction between FGF2 and glypican-1. The overexpression of glypican-1 also increased differentiation but did not affect proliferation when compared with the 1-chain, no-chain mutants, and the pCMS-EGFP vector without an insert. To support the overexpression data, glypican-1 expression was reduced using a small interfering RNA against turkey glypican-1. Inhibition of glypican-1 expression decreased myogenic satellite cell proliferation, differentiation, and FGF2 responsiveness during proliferation. These data indicate that glypican-1 function requires the GAG chain attachment sites for myogenic satellite cell FGF2 responsiveness during proliferation and to affect the process of differentiation. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/17704393/The_effect_of_glypican_1_glycosaminoglycan_chains_on_turkey_myogenic_satellite_cell_proliferation_differentiation_and_fibroblast_growth_factor_2_responsiveness_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)39231-4 DB - PRIME DP - Unbound Medicine ER -