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Recent advances using green and red fluorescent protein variants.
Appl Microbiol Biotechnol. 2007 Nov; 77(1):1-12.AM

Abstract

Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to green fluorescent protein (GFP) and its variants, recent progress has led to the development of monomeric red fluorescent proteins (mRFPs) that show improved properties with respect to maturation, brightness, and the monomeric state. This review considers green and red spectral variants, their paired use for live-cell imaging in vivo, in vitro, and in fluorescence resonance energy transfer (FRET) studies, in addition to other recent "two-color" advances including photoswitching and bimolecular fluorescence complementation (BiFC). It will be seen that green and red fluorescent proteins now exist with nearly ideal properties for dual-color microscopy and FRET.

Authors+Show Affiliations

Institut für Zellbiologie (ABI), Ludwig-Maximilians-Universität München, Schillerstrasse 42, Munich, Germany. amueller@lrz.uni-muenchen.deNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

17704916

Citation

Müller-Taubenberger, Annette, and Kurt I. Anderson. "Recent Advances Using Green and Red Fluorescent Protein Variants." Applied Microbiology and Biotechnology, vol. 77, no. 1, 2007, pp. 1-12.
Müller-Taubenberger A, Anderson KI. Recent advances using green and red fluorescent protein variants. Appl Microbiol Biotechnol. 2007;77(1):1-12.
Müller-Taubenberger, A., & Anderson, K. I. (2007). Recent advances using green and red fluorescent protein variants. Applied Microbiology and Biotechnology, 77(1), 1-12.
Müller-Taubenberger A, Anderson KI. Recent Advances Using Green and Red Fluorescent Protein Variants. Appl Microbiol Biotechnol. 2007;77(1):1-12. PubMed PMID: 17704916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recent advances using green and red fluorescent protein variants. AU - Müller-Taubenberger,Annette, AU - Anderson,Kurt I, Y1 - 2007/08/18/ PY - 2007/05/30/received PY - 2007/07/14/accepted PY - 2007/07/12/revised PY - 2007/8/21/pubmed PY - 2008/1/9/medline PY - 2007/8/21/entrez SP - 1 EP - 12 JF - Applied microbiology and biotechnology JO - Appl. Microbiol. Biotechnol. VL - 77 IS - 1 N2 - Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to green fluorescent protein (GFP) and its variants, recent progress has led to the development of monomeric red fluorescent proteins (mRFPs) that show improved properties with respect to maturation, brightness, and the monomeric state. This review considers green and red spectral variants, their paired use for live-cell imaging in vivo, in vitro, and in fluorescence resonance energy transfer (FRET) studies, in addition to other recent "two-color" advances including photoswitching and bimolecular fluorescence complementation (BiFC). It will be seen that green and red fluorescent proteins now exist with nearly ideal properties for dual-color microscopy and FRET. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/17704916/Recent_advances_using_green_and_red_fluorescent_protein_variants_ L2 - https://dx.doi.org/10.1007/s00253-007-1131-5 DB - PRIME DP - Unbound Medicine ER -