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Growth on ethanol results in co-ordinated Saccharomyces cerevisiae response to inactivation of genes encoding superoxide dismutases.
Redox Rep. 2007; 12(4):181-8.RR

Abstract

Superoxide dismutase (SOD) is an essential enzyme protecting cells against oxidative stress. However, its specific role under different conditions is not clear. To study the possible role of SOD in the cell during respiration, Saccharomyces cerevisiae single and double mutants with inactivated SOD1 and/or SOD2 genes growing on ethanol as an energy and carbon source were used. Activities of antioxidant and associated enzymes as well as the level of protein carbonyls were measured. SOD activity was significantly higher in a Mn-SOD deficient strain than that in the wild-type parental strain, but significantly lower in a Cu, Zn-SOD mutant. A strong positive correlation between SOD and catalase activities (R(2) = 0.99) shows possible protection of catalase by SOD from inactivation in vivo and/or decrease in catalase activity because of lower H(2)O(2) formation in the mutant cells. SOD deficiency resulted in a malate dehydrogenase activity increase, whereas glucose-6-phosphate dehydrogenase (G6PDH) activity was lower in SOD-deficient strains. Linear and non-linear positive correlations between SOD and isocitrate dehydrogenase activities are discussed. No changes in the activity of glutathione reductase and protein carbonyl levels support the idea that SOD-deficient cells are not exposed to strong oxidative stress during exponential growth of yeast cultures on ethanol.

Authors+Show Affiliations

Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine. lushchak@pu.if.ua <lushchak@pu.if.ua>No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17705988

Citation

Lushchak, Oleh V., et al. "Growth On Ethanol Results in Co-ordinated Saccharomyces Cerevisiae Response to Inactivation of Genes Encoding Superoxide Dismutases." Redox Report : Communications in Free Radical Research, vol. 12, no. 4, 2007, pp. 181-8.
Lushchak OV, Semchyshyn HM, Lushchak VI. Growth on ethanol results in co-ordinated Saccharomyces cerevisiae response to inactivation of genes encoding superoxide dismutases. Redox Rep. 2007;12(4):181-8.
Lushchak, O. V., Semchyshyn, H. M., & Lushchak, V. I. (2007). Growth on ethanol results in co-ordinated Saccharomyces cerevisiae response to inactivation of genes encoding superoxide dismutases. Redox Report : Communications in Free Radical Research, 12(4), 181-8.
Lushchak OV, Semchyshyn HM, Lushchak VI. Growth On Ethanol Results in Co-ordinated Saccharomyces Cerevisiae Response to Inactivation of Genes Encoding Superoxide Dismutases. Redox Rep. 2007;12(4):181-8. PubMed PMID: 17705988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth on ethanol results in co-ordinated Saccharomyces cerevisiae response to inactivation of genes encoding superoxide dismutases. AU - Lushchak,Oleh V, AU - Semchyshyn,Halyna M, AU - Lushchak,Volodymyr I, PY - 2007/8/21/pubmed PY - 2007/10/30/medline PY - 2007/8/21/entrez SP - 181 EP - 8 JF - Redox report : communications in free radical research JO - Redox Rep VL - 12 IS - 4 N2 - Superoxide dismutase (SOD) is an essential enzyme protecting cells against oxidative stress. However, its specific role under different conditions is not clear. To study the possible role of SOD in the cell during respiration, Saccharomyces cerevisiae single and double mutants with inactivated SOD1 and/or SOD2 genes growing on ethanol as an energy and carbon source were used. Activities of antioxidant and associated enzymes as well as the level of protein carbonyls were measured. SOD activity was significantly higher in a Mn-SOD deficient strain than that in the wild-type parental strain, but significantly lower in a Cu, Zn-SOD mutant. A strong positive correlation between SOD and catalase activities (R(2) = 0.99) shows possible protection of catalase by SOD from inactivation in vivo and/or decrease in catalase activity because of lower H(2)O(2) formation in the mutant cells. SOD deficiency resulted in a malate dehydrogenase activity increase, whereas glucose-6-phosphate dehydrogenase (G6PDH) activity was lower in SOD-deficient strains. Linear and non-linear positive correlations between SOD and isocitrate dehydrogenase activities are discussed. No changes in the activity of glutathione reductase and protein carbonyl levels support the idea that SOD-deficient cells are not exposed to strong oxidative stress during exponential growth of yeast cultures on ethanol. SN - 1743-2928 UR - https://www.unboundmedicine.com/medline/citation/17705988/Growth_on_ethanol_results_in_co_ordinated_Saccharomyces_cerevisiae_response_to_inactivation_of_genes_encoding_superoxide_dismutases_ L2 - https://www.tandfonline.com/doi/full/10.1179/135100007X200263 DB - PRIME DP - Unbound Medicine ER -