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Increased frequency of human leukocyte antigen-E inhibitory receptor CD94/NKG2A-expressing peritoneal natural killer cells in patients with endometriosis.
Fertil Steril. 2008 May; 89(5 Suppl):1490-6.FS

Abstract

OBJECTIVE

To analyze the frequency of peritoneal natural killer (NK) cells expressing the human leukocyte antigen (HLA)-E receptor CD94/NKG2A in patients with endometriosis.

DESIGN

Case-control study.

SETTING

University hospital.

PATIENT(S)

Stage III and stage IV endometriosis, according to the revised American Society for Reproductive Medicine classification, was laparoscopically and histologically confirmed in 11 and 9 patients, respectively; 13 subjects without endometriosis were selected for the control group.

INTERVENTION(S)

Collection of peripheral venous blood, peritoneal fluid, endometriotic tissue, and normal endometrium in subjects undergoing laparoscopy.

MAIN OUTCOME MEASURE(S)

Surface expression levels of CD94/NKG2A and CD94/NKG2C were detected by three-color cytofluorometric analysis. Semiquantitative HLA-E messenger RNA expression analysis was performed in endometriotic lesions and in eutopic endometrium. NK cell-mediated cytotoxic activity toward HLA-E positive target, DT360 cell line, was also determined.

RESULT(S)

In women with endometriosis, the percentage of CD94/NKG2A-positive peritoneal NK cells was significantly higher than in the control group. The CD94/NKG2A ligand, HLA-E, was detected at high levels in endometriotic tissue as messenger RNA transcript. Target cells bearing HLA-E were resistant to NK cell-mediated lysis in a CD94/NKG2A-dependent manner.

CONCLUSION(S)

Increased expression of CD94/NKG2A in peritoneal NK cells may mediate the resistance of endometriotic tissue to NK cell-mediated lysis, thus contributing to the progression of the disease.

Authors+Show Affiliations

Department of Experimental Medicine, University "La Sapienza," Rome, Italy. ricciarda.galandrini@uniroma1.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17706207

Citation

Galandrini, Ricciarda, et al. "Increased Frequency of Human Leukocyte antigen-E Inhibitory Receptor CD94/NKG2A-expressing Peritoneal Natural Killer Cells in Patients With Endometriosis." Fertility and Sterility, vol. 89, no. 5 Suppl, 2008, pp. 1490-6.
Galandrini R, Porpora MG, Stoppacciaro A, et al. Increased frequency of human leukocyte antigen-E inhibitory receptor CD94/NKG2A-expressing peritoneal natural killer cells in patients with endometriosis. Fertil Steril. 2008;89(5 Suppl):1490-6.
Galandrini, R., Porpora, M. G., Stoppacciaro, A., Micucci, F., Capuano, C., Tassi, I., Di Felice, A., Benedetti-Panici, P., & Santoni, A. (2008). Increased frequency of human leukocyte antigen-E inhibitory receptor CD94/NKG2A-expressing peritoneal natural killer cells in patients with endometriosis. Fertility and Sterility, 89(5 Suppl), 1490-6.
Galandrini R, et al. Increased Frequency of Human Leukocyte antigen-E Inhibitory Receptor CD94/NKG2A-expressing Peritoneal Natural Killer Cells in Patients With Endometriosis. Fertil Steril. 2008;89(5 Suppl):1490-6. PubMed PMID: 17706207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased frequency of human leukocyte antigen-E inhibitory receptor CD94/NKG2A-expressing peritoneal natural killer cells in patients with endometriosis. AU - Galandrini,Ricciarda, AU - Porpora,Maria Grazia, AU - Stoppacciaro,Antonella, AU - Micucci,Federica, AU - Capuano,Cristina, AU - Tassi,Ilaria, AU - Di Felice,Alessia, AU - Benedetti-Panici,Pierluigi, AU - Santoni,Angela, Y1 - 2007/08/13/ PY - 2006/11/22/received PY - 2007/05/04/revised PY - 2007/05/04/accepted PY - 2007/8/21/pubmed PY - 2008/7/17/medline PY - 2007/8/21/entrez SP - 1490 EP - 6 JF - Fertility and sterility JO - Fertil. Steril. VL - 89 IS - 5 Suppl N2 - OBJECTIVE: To analyze the frequency of peritoneal natural killer (NK) cells expressing the human leukocyte antigen (HLA)-E receptor CD94/NKG2A in patients with endometriosis. DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Stage III and stage IV endometriosis, according to the revised American Society for Reproductive Medicine classification, was laparoscopically and histologically confirmed in 11 and 9 patients, respectively; 13 subjects without endometriosis were selected for the control group. INTERVENTION(S): Collection of peripheral venous blood, peritoneal fluid, endometriotic tissue, and normal endometrium in subjects undergoing laparoscopy. MAIN OUTCOME MEASURE(S): Surface expression levels of CD94/NKG2A and CD94/NKG2C were detected by three-color cytofluorometric analysis. Semiquantitative HLA-E messenger RNA expression analysis was performed in endometriotic lesions and in eutopic endometrium. NK cell-mediated cytotoxic activity toward HLA-E positive target, DT360 cell line, was also determined. RESULT(S): In women with endometriosis, the percentage of CD94/NKG2A-positive peritoneal NK cells was significantly higher than in the control group. The CD94/NKG2A ligand, HLA-E, was detected at high levels in endometriotic tissue as messenger RNA transcript. Target cells bearing HLA-E were resistant to NK cell-mediated lysis in a CD94/NKG2A-dependent manner. CONCLUSION(S): Increased expression of CD94/NKG2A in peritoneal NK cells may mediate the resistance of endometriotic tissue to NK cell-mediated lysis, thus contributing to the progression of the disease. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17706207/Increased_frequency_of_human_leukocyte_antigen_E_inhibitory_receptor_CD94/NKG2A_expressing_peritoneal_natural_killer_cells_in_patients_with_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)01143-0 DB - PRIME DP - Unbound Medicine ER -