Tags

Type your tag names separated by a space and hit enter

GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro.
Food Chem Toxicol. 2007 Dec; 45(12):2592-6.FC

Abstract

Antioxidants are of major importance in the protection against cellular oxidative damage caused by endogenous as well as exogenous free radicals. This study aims to establish the impact of genetic polymorphisms in GSTM1 and GSTT1, which encode for enzymatic antioxidative defence, on H(2)O(2)-induced oxidative DNA damage and on the effectiveness of quercetin and ascorbic acid in preventing this induced damage in human lymphocytes. Lymphocytes from 12 healthy volunteers were pre-incubated either with 10 microM of quercetin or with 10 microM of ascorbic acid, and exposed to 25 microM H(2)O(2) for 1h. The induction of oxidative DNA damage was quantified using the Comet assay. Genotyping of these 12 subjects showed that six individuals were GSTM1+ and six were GSTM1-; eight were GSTT1+ and four GSTT1-.

RESULTS

Baseline levels of oxidative DNA damage did not differ between GSTM1 or GSTT1 variants and their respective wild types. Also with respect to ex vivo induced levels of oxidative DNA damage, no significant difference was seen between variants and wild types of both genotypes. The protection against H(2)O(2)-induced oxidative DNA damage by quercetin was significantly higher in GSTT1 wild types than in GSTT1 variants (57% and 9% decrease, respectively; p=0.01); furthermore, GSTT1 wild types were protected against induced oxidative DNA damage by ascorbic acid pre-incubation while GSTT1 variants showed an increase of damage (16% decrease vs. 91% increase; p=0.01). For GSTM1 variants and wild types, observed differences in protective effects of quercetin or ascorbic acid were not statistically significant. Overall, quercetin proves to be better in protecting human lymphocytes in vitro against oxidative DNA damage upon H(2)O(2) challenge than ascorbic acid.

Links

Publisher Full Text

Authors+Show Affiliations

Wilms LC
Department of Health Risk Analysis and Toxicology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. L.Wilms@GRAT.unimaas.nl
Claughton TA
No affiliation info available
de Kok TM
No affiliation info available
Kleinjans JC
No affiliation info available

MeSH

AdultAntioxidantsAscorbic AcidDNA DamageFemaleGlutathione TransferaseHumansHydrogen PeroxideIn Vitro TechniquesLymphocytesMaleMiddle AgedPhytotherapyPolymorphism, GeneticQuercetin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17706336

Citation

Wilms, Lonneke C., et al. "GSTM1 and GSTT1 Polymorphism Influences Protection Against Induced Oxidative DNA Damage By Quercetin and Ascorbic Acid in Human Lymphocytes in Vitro." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 45, no. 12, 2007, pp. 2592-6.
Wilms LC, Claughton TA, de Kok TM, et al. GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro. Food Chem Toxicol. 2007;45(12):2592-6.
Wilms, L. C., Claughton, T. A., de Kok, T. M., & Kleinjans, J. C. (2007). GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 45(12), 2592-6.
Wilms LC, et al. GSTM1 and GSTT1 Polymorphism Influences Protection Against Induced Oxidative DNA Damage By Quercetin and Ascorbic Acid in Human Lymphocytes in Vitro. Food Chem Toxicol. 2007;45(12):2592-6. PubMed PMID: 17706336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro. AU - Wilms,Lonneke C, AU - Claughton,Tamara-Ann, AU - de Kok,Theo M C M, AU - Kleinjans,Jos C S, Y1 - 2007/06/30/ PY - 2006/04/04/received PY - 2007/01/26/revised PY - 2007/06/25/accepted PY - 2007/8/21/pubmed PY - 2007/12/14/medline PY - 2007/8/21/entrez SP - 2592 EP - 6 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 45 IS - 12 N2 - UNLABELLED: Antioxidants are of major importance in the protection against cellular oxidative damage caused by endogenous as well as exogenous free radicals. This study aims to establish the impact of genetic polymorphisms in GSTM1 and GSTT1, which encode for enzymatic antioxidative defence, on H(2)O(2)-induced oxidative DNA damage and on the effectiveness of quercetin and ascorbic acid in preventing this induced damage in human lymphocytes. Lymphocytes from 12 healthy volunteers were pre-incubated either with 10 microM of quercetin or with 10 microM of ascorbic acid, and exposed to 25 microM H(2)O(2) for 1h. The induction of oxidative DNA damage was quantified using the Comet assay. Genotyping of these 12 subjects showed that six individuals were GSTM1+ and six were GSTM1-; eight were GSTT1+ and four GSTT1-. RESULTS: Baseline levels of oxidative DNA damage did not differ between GSTM1 or GSTT1 variants and their respective wild types. Also with respect to ex vivo induced levels of oxidative DNA damage, no significant difference was seen between variants and wild types of both genotypes. The protection against H(2)O(2)-induced oxidative DNA damage by quercetin was significantly higher in GSTT1 wild types than in GSTT1 variants (57% and 9% decrease, respectively; p=0.01); furthermore, GSTT1 wild types were protected against induced oxidative DNA damage by ascorbic acid pre-incubation while GSTT1 variants showed an increase of damage (16% decrease vs. 91% increase; p=0.01). For GSTM1 variants and wild types, observed differences in protective effects of quercetin or ascorbic acid were not statistically significant. Overall, quercetin proves to be better in protecting human lymphocytes in vitro against oxidative DNA damage upon H(2)O(2) challenge than ascorbic acid. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/17706336/GSTM1_and_GSTT1_polymorphism_influences_protection_against_induced_oxidative_DNA_damage_by_quercetin_and_ascorbic_acid_in_human_lymphocytes_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(07)00246-3 DB - PRIME DP - Unbound Medicine ER -