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Isolation and sequencing of two FMRFamide-related peptides from the gut of Locusta migratoria L.
Peptides. 2007 Aug; 28(8):1490-7.P

Abstract

Two FMRFamide-related peptides (FaRPs) have been isolated and sequenced from the whole gut of Locusta migratoria L. Peptides were extracted from 500 locust whole guts and separated using reversed-phase high performance liquid chromatography (RP-HPLC). Fractions containing FMRFamide-like immunoreactive (FLI) material were identified using radioimmunoassay (RIA). Sequencing of fractions, using tandem mass spectrometry (MALDI-TOF MS/MS), revealed the myosuppressin previously isolated from the locust CNS, SchistoFLRFamide (PDVDHVFLRFamide), and a novel extended RFamide (LWENLRFamide). The isolation of SchistoFLRFamide from midgut tissue supports the hypothesis that this myosuppressin is released locally from FLI processes over the gut and/or from endocrine-like midgut cells to play a role in the regulation of digestion.

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Authors+Show Affiliations

Hill SR
Division of Chemical Ecology, Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden. sharon.hill@ltj.slu.se
Orchard I
No affiliation info available

MeSH

Amino Acid SequenceAnimalsChromatography, High Pressure LiquidDigestive SystemFMRFamideIn Vitro TechniquesInsect ProteinsLocusta migratoriaMuscle ContractionNeuropeptidesSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17707763

Citation

Hill, Sharon R., and Ian Orchard. "Isolation and Sequencing of Two FMRFamide-related Peptides From the Gut of Locusta Migratoria L." Peptides, vol. 28, no. 8, 2007, pp. 1490-7.
Hill SR, Orchard I. Isolation and sequencing of two FMRFamide-related peptides from the gut of Locusta migratoria L. Peptides. 2007;28(8):1490-7.
Hill, S. R., & Orchard, I. (2007). Isolation and sequencing of two FMRFamide-related peptides from the gut of Locusta migratoria L. Peptides, 28(8), 1490-7.
Hill SR, Orchard I. Isolation and Sequencing of Two FMRFamide-related Peptides From the Gut of Locusta Migratoria L. Peptides. 2007;28(8):1490-7. PubMed PMID: 17707763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and sequencing of two FMRFamide-related peptides from the gut of Locusta migratoria L. AU - Hill,Sharon R, AU - Orchard,Ian, Y1 - 2007/06/22/ PY - 2006/08/16/received PY - 2007/06/02/revised PY - 2007/06/04/accepted PY - 2007/8/21/pubmed PY - 2007/11/9/medline PY - 2007/8/21/entrez SP - 1490 EP - 7 JF - Peptides JO - Peptides VL - 28 IS - 8 N2 - Two FMRFamide-related peptides (FaRPs) have been isolated and sequenced from the whole gut of Locusta migratoria L. Peptides were extracted from 500 locust whole guts and separated using reversed-phase high performance liquid chromatography (RP-HPLC). Fractions containing FMRFamide-like immunoreactive (FLI) material were identified using radioimmunoassay (RIA). Sequencing of fractions, using tandem mass spectrometry (MALDI-TOF MS/MS), revealed the myosuppressin previously isolated from the locust CNS, SchistoFLRFamide (PDVDHVFLRFamide), and a novel extended RFamide (LWENLRFamide). The isolation of SchistoFLRFamide from midgut tissue supports the hypothesis that this myosuppressin is released locally from FLI processes over the gut and/or from endocrine-like midgut cells to play a role in the regulation of digestion. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/17707763/Isolation_and_sequencing_of_two_FMRFamide_related_peptides_from_the_gut_of_Locusta_migratoria_L_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(07)00199-4 DB - PRIME DP - Unbound Medicine ER -