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Metabolic characterization of Brassica rapa leaves by NMR spectroscopy.
J Agric Food Chem. 2007 Sep 19; 55(19):7936-43.JA

Abstract

The Brassica has been intensively studied due to the nutritional and beneficial effects. However, many species, varieties, and cultivars of this genus and the resulting large metabolic variation have been obstacles for systematic research of the plant. In order to overcome the problems posed by the biological variation, the metabolomic analysis of various cultivars of Brassica rapa was performed by NMR spectroscopy combined with multivariate data analysis. Discriminating metabolites in different cultivars and development stages were elucidated by diverse 2D-NMR techniques after sorting out different significant signals using (1)H NMR measurements and principal component analysis. Among the elucidated metabolites, several organic and amino acids, carbohydrates, adenine, indole acetic acid (IAA), phenylpropanoids, flavonoids, and glucosinolates were found to be the metabolites contributing to the differentiation between cultivars and age of Brassica rapa. On the basis of these results, the distribution of plant metabolites among different cultivars and development stages of B. rapa is discussed.

Authors+Show Affiliations

Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17708639

Citation

Abdel-Farid, Ibrahim Bayoumi, et al. "Metabolic Characterization of Brassica Rapa Leaves By NMR Spectroscopy." Journal of Agricultural and Food Chemistry, vol. 55, no. 19, 2007, pp. 7936-43.
Abdel-Farid IB, Kim HK, Choi YH, et al. Metabolic characterization of Brassica rapa leaves by NMR spectroscopy. J Agric Food Chem. 2007;55(19):7936-43.
Abdel-Farid, I. B., Kim, H. K., Choi, Y. H., & Verpoorte, R. (2007). Metabolic characterization of Brassica rapa leaves by NMR spectroscopy. Journal of Agricultural and Food Chemistry, 55(19), 7936-43.
Abdel-Farid IB, et al. Metabolic Characterization of Brassica Rapa Leaves By NMR Spectroscopy. J Agric Food Chem. 2007 Sep 19;55(19):7936-43. PubMed PMID: 17708639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic characterization of Brassica rapa leaves by NMR spectroscopy. AU - Abdel-Farid,Ibrahim Bayoumi, AU - Kim,Hye Kyong, AU - Choi,Young Hae, AU - Verpoorte,Robert, Y1 - 2007/08/21/ PY - 2007/8/22/pubmed PY - 2007/12/13/medline PY - 2007/8/22/entrez SP - 7936 EP - 43 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 19 N2 - The Brassica has been intensively studied due to the nutritional and beneficial effects. However, many species, varieties, and cultivars of this genus and the resulting large metabolic variation have been obstacles for systematic research of the plant. In order to overcome the problems posed by the biological variation, the metabolomic analysis of various cultivars of Brassica rapa was performed by NMR spectroscopy combined with multivariate data analysis. Discriminating metabolites in different cultivars and development stages were elucidated by diverse 2D-NMR techniques after sorting out different significant signals using (1)H NMR measurements and principal component analysis. Among the elucidated metabolites, several organic and amino acids, carbohydrates, adenine, indole acetic acid (IAA), phenylpropanoids, flavonoids, and glucosinolates were found to be the metabolites contributing to the differentiation between cultivars and age of Brassica rapa. On the basis of these results, the distribution of plant metabolites among different cultivars and development stages of B. rapa is discussed. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17708639/Metabolic_characterization_of_Brassica_rapa_leaves_by_NMR_spectroscopy_ L2 - https://doi.org/10.1021/jf071294b DB - PRIME DP - Unbound Medicine ER -