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In vitro mating of Colombian isolates of the Cryptococcus neoformans species complex.
Biomedica. 2007 Jun; 27(2):308-14.B

Abstract

INTRODUCTION

Within the Cryptococcus neoformans species complex, two species and five serotypes are recognized: C. neoformans (var. grubii, serotype A; var. neoformans, serotype D and a hybrid, serotype AD) and C. gattii (serotypes B and C). Mating types a and alpha are designated by a single locus, with the mating type alpha being most prevalent in serotype A and D strains.

OBJECTIVE

To evaluate the ability of Colombian isolates of the C. neoformans species complex to mate in vitro with tester strains of the opposite mating type.

MATERIALS AND METHODS

Fifty three clinical isolates were included in this study, 33 C. neoformans var. grubii serotype A, 4 C. neoformans var. neoformans serotype D, all mating type alpha, and 16 C. gattii, 13 serotype B (mating type a) and 3 serotype C (mating type alpha), were mixed on V8 juice agar, using a modified method, with the appropriate tester strains to determine the mating types in vitro.

RESULTS

Mating studies revealed that 9 of 33 (27.3%) serotype A isolates and 6 of 13 (46.2%) serotype B isolates were able to mate. Clamp connections and basidia with basidiospores were observed microscopically, indicating that the mating process had occurred. All mating competent serotype A strains were mating type alpha and the serotype B mating competent strains were mating type a.

CONCLUSION

This is the first report of the determination of the mating ability of Colombian Cryptococcus neoformans isolates to mate in vitro with appropriate tester strains, which is of great importance to study the propagation of the fungus around the globe.

Authors+Show Affiliations

Escandón P
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, D. C, Colombia.
Ngamskulrungroj P
No affiliation info available
Meyer W
No affiliation info available
Castañeda E
No affiliation info available

MeSH

ColombiaCryptococcosisCryptococcus neoformansGenes, Mating Type, FungalHumansMycological Typing TechniquesSerotyping

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17713641

Citation

Escandón, Patricia, et al. "In Vitro Mating of Colombian Isolates of the Cryptococcus Neoformans Species Complex." Biomedica : Revista Del Instituto Nacional De Salud, vol. 27, no. 2, 2007, pp. 308-14.
Escandón P, Ngamskulrungroj P, Meyer W, et al. In vitro mating of Colombian isolates of the Cryptococcus neoformans species complex. Biomedica. 2007;27(2):308-14.
Escandón, P., Ngamskulrungroj, P., Meyer, W., & Castañeda, E. (2007). In vitro mating of Colombian isolates of the Cryptococcus neoformans species complex. Biomedica : Revista Del Instituto Nacional De Salud, 27(2), 308-14.
Escandón P, et al. In Vitro Mating of Colombian Isolates of the Cryptococcus Neoformans Species Complex. Biomedica. 2007;27(2):308-14. PubMed PMID: 17713641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro mating of Colombian isolates of the Cryptococcus neoformans species complex. AU - Escandón,Patricia, AU - Ngamskulrungroj,Popchai, AU - Meyer,Wieland, AU - Castañeda,Elizabeth, Y1 - 2007/08/21/ PY - 2007/8/24/pubmed PY - 2008/2/12/medline PY - 2007/8/24/entrez SP - 308 EP - 14 JF - Biomedica : revista del Instituto Nacional de Salud JO - Biomedica VL - 27 IS - 2 N2 - INTRODUCTION: Within the Cryptococcus neoformans species complex, two species and five serotypes are recognized: C. neoformans (var. grubii, serotype A; var. neoformans, serotype D and a hybrid, serotype AD) and C. gattii (serotypes B and C). Mating types a and alpha are designated by a single locus, with the mating type alpha being most prevalent in serotype A and D strains. OBJECTIVE: To evaluate the ability of Colombian isolates of the C. neoformans species complex to mate in vitro with tester strains of the opposite mating type. MATERIALS AND METHODS: Fifty three clinical isolates were included in this study, 33 C. neoformans var. grubii serotype A, 4 C. neoformans var. neoformans serotype D, all mating type alpha, and 16 C. gattii, 13 serotype B (mating type a) and 3 serotype C (mating type alpha), were mixed on V8 juice agar, using a modified method, with the appropriate tester strains to determine the mating types in vitro. RESULTS: Mating studies revealed that 9 of 33 (27.3%) serotype A isolates and 6 of 13 (46.2%) serotype B isolates were able to mate. Clamp connections and basidia with basidiospores were observed microscopically, indicating that the mating process had occurred. All mating competent serotype A strains were mating type alpha and the serotype B mating competent strains were mating type a. CONCLUSION: This is the first report of the determination of the mating ability of Colombian Cryptococcus neoformans isolates to mate in vitro with appropriate tester strains, which is of great importance to study the propagation of the fungus around the globe. SN - 0120-4157 UR - https://www.unboundmedicine.com/medline/citation/17713641/In_vitro_mating_of_Colombian_isolates_of_the_Cryptococcus_neoformans_species_complex_ DB - PRIME DP - Unbound Medicine ER -