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Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products.
J Appl Microbiol. 2007 Sep; 103(3):666-74.JA

Abstract

AIMS

Considering the significant rise in the probiotic market in Columbia, and given the lack of reports concerning the microbial population and strain performance in products from different producers, this study aims at determining the number of viable starter bacteria and probiotics in bio-yoghurts available at the Columbian market, identifying the species and analysing the performance of the isolated strains in bile acid resistance, antagonistic activity against pathogens, and adherence capacity to human intestinal epithelial cells.

METHODS AND RESULTS

Seven bio-yoghurts were analysed for the bacterial species present. Species identification was carried out using 16S rRNA gene targeted PCR. The cultured bacteria were tested for bile acid resistance, adherence to a human intestinal epithelial cell line, and antagonism against the pathogen Salmonella enterica serovar Typhimurium. A total of 17 different strains were identified. Based on plate counting, all bio-yoghurts have at least total viable cells of approximately 10(7) CFU ml(-1). Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus were the most frequently isolated bacteria. Viable Bifidobacterium was only recovered from one product. However, after PCR analysis, DNA of this genus was confirmed in five out of seven products. Major differences were found for S. typhimurium antagonism. The adherence capacity to Caco-2 cells was observed in 10 of the isolated strains. In general, low survival to simulated gastric juice was observed.

CONCLUSIONS

Some of the isolated strains have probiotic potential, although not all of them were present in the advised amount to exert beneficial health effects. However, the full correct scientific name of the isolated bacteria and their viable counts were not included on the product label.

SIGNIFICANCE AND IMPACT OF THE STUDY

This is the first report describing the identification and functionality of starter bacteria and probiotics present in dairy products on the Columbian market.

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Authors+Show Affiliations

Perea Vélez M
Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Leuven, Belgium.
Hermans K
No affiliation info available
Verhoeven TL
No affiliation info available
Lebeer SE
No affiliation info available
Vanderleyden J
No affiliation info available
De Keersmaecker SC
No affiliation info available

MeSH

Bacterial AdhesionBifidobacteriumCaco-2 CellsColombiaColony Count, MicrobialFood HandlingFood MicrobiologyGastric JuiceHumansLactobacillusLactobacillus delbrueckiiProbioticsSalmonella typhimuriumStreptococcus thermophilusYogurt

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17714400

Citation

Perea Vélez, M, et al. "Identification and Characterization of Starter Lactic Acid Bacteria and Probiotics From Columbian Dairy Products." Journal of Applied Microbiology, vol. 103, no. 3, 2007, pp. 666-74.
Perea Vélez M, Hermans K, Verhoeven TL, et al. Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. J Appl Microbiol. 2007;103(3):666-74.
Perea Vélez, M., Hermans, K., Verhoeven, T. L., Lebeer, S. E., Vanderleyden, J., & De Keersmaecker, S. C. (2007). Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. Journal of Applied Microbiology, 103(3), 666-74.
Perea Vélez M, et al. Identification and Characterization of Starter Lactic Acid Bacteria and Probiotics From Columbian Dairy Products. J Appl Microbiol. 2007;103(3):666-74. PubMed PMID: 17714400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. AU - Perea Vélez,M, AU - Hermans,K, AU - Verhoeven,T L A, AU - Lebeer,S E, AU - Vanderleyden,J, AU - De Keersmaecker,S C J, PY - 2007/8/24/pubmed PY - 2007/12/28/medline PY - 2007/8/24/entrez SP - 666 EP - 74 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 103 IS - 3 N2 - AIMS: Considering the significant rise in the probiotic market in Columbia, and given the lack of reports concerning the microbial population and strain performance in products from different producers, this study aims at determining the number of viable starter bacteria and probiotics in bio-yoghurts available at the Columbian market, identifying the species and analysing the performance of the isolated strains in bile acid resistance, antagonistic activity against pathogens, and adherence capacity to human intestinal epithelial cells. METHODS AND RESULTS: Seven bio-yoghurts were analysed for the bacterial species present. Species identification was carried out using 16S rRNA gene targeted PCR. The cultured bacteria were tested for bile acid resistance, adherence to a human intestinal epithelial cell line, and antagonism against the pathogen Salmonella enterica serovar Typhimurium. A total of 17 different strains were identified. Based on plate counting, all bio-yoghurts have at least total viable cells of approximately 10(7) CFU ml(-1). Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus were the most frequently isolated bacteria. Viable Bifidobacterium was only recovered from one product. However, after PCR analysis, DNA of this genus was confirmed in five out of seven products. Major differences were found for S. typhimurium antagonism. The adherence capacity to Caco-2 cells was observed in 10 of the isolated strains. In general, low survival to simulated gastric juice was observed. CONCLUSIONS: Some of the isolated strains have probiotic potential, although not all of them were present in the advised amount to exert beneficial health effects. However, the full correct scientific name of the isolated bacteria and their viable counts were not included on the product label. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report describing the identification and functionality of starter bacteria and probiotics present in dairy products on the Columbian market. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/17714400/Identification_and_characterization_of_starter_lactic_acid_bacteria_and_probiotics_from_Columbian_dairy_products_ L2 - https://doi.org/10.1111/j.1365-2672.2007.03294.x DB - PRIME DP - Unbound Medicine ER -