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Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia.
Plant Biotechnol J. 2007 Nov; 5(6):746-58.PB

Abstract

Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lavender plants expressing the Arabidopsis thaliana HMG1 cDNA, encoding the catalytic domain of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR1S), a key enzyme of the mevalonic acid (MVA) pathway. Transgenic T0 plants accumulated significantly more essential oil constituents as compared to controls (up to 2.1- and 1.8-fold in leaves and flowers, respectively). Enhanced expression of HMGR1S also increased the amount of the end-product sterols, beta-sitosterol and stigmasterol (average differences of 1.8- and 1.9-fold, respectively), but did not affect the accumulation of carotenoids or chlorophylls. We also analysed T1 plants derived from self-pollinated seeds of T0 lines that flowered after growing for 2 years in the greenhouse. The increased levels of essential oil and sterols observed in the transgenic T0 plants were maintained in the progeny that inherited the HMG1 transgene. Our results demonstrate that genetic manipulation of the MVA pathway increases essential oil yield in spike lavender, suggesting a contribution for this cytosolic pathway to monoterpene and sesquiterpene biosynthesis in leaves and flowers of the species.

Authors+Show Affiliations

Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, 46100 Burjasot, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17714440

Citation

Muñoz-Bertomeu, Jesús, et al. "Up-regulation of an N-terminal Truncated 3-hydroxy-3-methylglutaryl CoA Reductase Enhances Production of Essential Oils and Sterols in Transgenic Lavandula Latifolia." Plant Biotechnology Journal, vol. 5, no. 6, 2007, pp. 746-58.
Muñoz-Bertomeu J, Sales E, Ros R, et al. Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia. Plant Biotechnol J. 2007;5(6):746-58.
Muñoz-Bertomeu, J., Sales, E., Ros, R., Arrillaga, I., & Segura, J. (2007). Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia. Plant Biotechnology Journal, 5(6), 746-58.
Muñoz-Bertomeu J, et al. Up-regulation of an N-terminal Truncated 3-hydroxy-3-methylglutaryl CoA Reductase Enhances Production of Essential Oils and Sterols in Transgenic Lavandula Latifolia. Plant Biotechnol J. 2007;5(6):746-58. PubMed PMID: 17714440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia. AU - Muñoz-Bertomeu,Jesús, AU - Sales,Ester, AU - Ros,Roc, AU - Arrillaga,Isabel, AU - Segura,Juan, Y1 - 2007/08/22/ PY - 2007/8/24/pubmed PY - 2007/12/28/medline PY - 2007/8/24/entrez SP - 746 EP - 58 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 5 IS - 6 N2 - Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lavender plants expressing the Arabidopsis thaliana HMG1 cDNA, encoding the catalytic domain of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR1S), a key enzyme of the mevalonic acid (MVA) pathway. Transgenic T0 plants accumulated significantly more essential oil constituents as compared to controls (up to 2.1- and 1.8-fold in leaves and flowers, respectively). Enhanced expression of HMGR1S also increased the amount of the end-product sterols, beta-sitosterol and stigmasterol (average differences of 1.8- and 1.9-fold, respectively), but did not affect the accumulation of carotenoids or chlorophylls. We also analysed T1 plants derived from self-pollinated seeds of T0 lines that flowered after growing for 2 years in the greenhouse. The increased levels of essential oil and sterols observed in the transgenic T0 plants were maintained in the progeny that inherited the HMG1 transgene. Our results demonstrate that genetic manipulation of the MVA pathway increases essential oil yield in spike lavender, suggesting a contribution for this cytosolic pathway to monoterpene and sesquiterpene biosynthesis in leaves and flowers of the species. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/17714440/Up_regulation_of_an_N_terminal_truncated_3_hydroxy_3_methylglutaryl_CoA_reductase_enhances_production_of_essential_oils_and_sterols_in_transgenic_Lavandula_latifolia_ L2 - https://doi.org/10.1111/j.1467-7652.2007.00286.x DB - PRIME DP - Unbound Medicine ER -