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Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR.
Clin Microbiol Infect. 2007 Oct; 13(10):1001-7.CM

Abstract

The diagnostic value of a multiplex real-time PCR for the detection of Entamoeba histolytica, Giardia lamblia and Cryptosporidium parvum/Cryptosporidium hominis was evaluated by comparing the PCR results obtained with those of routinely performed microscopy of faecal samples from patients consulting their general practitioner (GP) because of gastrointestinal complaints. Analysis of 722 faecal DNA samples revealed that the prevalence of G. lamblia was 9.3% according to PCR, as compared to 5.7% by microscopy. The number of infections detected was more than double in children of school age. Furthermore, G. lamblia infection was detected in 15 (6.6%) of 228 faecal samples submitted to the laboratory for bacterial culture only. C. parvum/C. hominis infections were not diagnosed by routine procedures, but DNA from these organisms was detected in 4.3% of 950 DNA samples. A strong association with age was noted, with Cryptosporidium being detected in 21.8% of 110 children aged <5 years. C. hominis was the most prevalent species. E. histolytica was not detected in this study population. Analysis of microscopy data revealed that the number of additional parasites missed by PCR was small. Overall, the study demonstrated that a multiplex real-time PCR approach is a feasible diagnostic alternative in the clinical laboratory for the detection of parasitic infections in patients consulting GPs because of gastrointestinal symptoms.

Authors+Show Affiliations

Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands. R.ten_Hove@lumc.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17714523

Citation

ten Hove, R, et al. "Detection of Diarrhoea-causing Protozoa in General Practice Patients in the Netherlands By Multiplex Real-time PCR." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 13, no. 10, 2007, pp. 1001-7.
ten Hove R, Schuurman T, Kooistra M, et al. Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR. Clin Microbiol Infect. 2007;13(10):1001-7.
ten Hove, R., Schuurman, T., Kooistra, M., Möller, L., van Lieshout, L., & Verweij, J. J. (2007). Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 13(10), 1001-7.
ten Hove R, et al. Detection of Diarrhoea-causing Protozoa in General Practice Patients in the Netherlands By Multiplex Real-time PCR. Clin Microbiol Infect. 2007;13(10):1001-7. PubMed PMID: 17714523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR. AU - ten Hove,R, AU - Schuurman,T, AU - Kooistra,M, AU - Möller,L, AU - van Lieshout,L, AU - Verweij,J J, Y1 - 2007/08/21/ PY - 2007/8/24/pubmed PY - 2007/12/7/medline PY - 2007/8/24/entrez SP - 1001 EP - 7 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin. Microbiol. Infect. VL - 13 IS - 10 N2 - The diagnostic value of a multiplex real-time PCR for the detection of Entamoeba histolytica, Giardia lamblia and Cryptosporidium parvum/Cryptosporidium hominis was evaluated by comparing the PCR results obtained with those of routinely performed microscopy of faecal samples from patients consulting their general practitioner (GP) because of gastrointestinal complaints. Analysis of 722 faecal DNA samples revealed that the prevalence of G. lamblia was 9.3% according to PCR, as compared to 5.7% by microscopy. The number of infections detected was more than double in children of school age. Furthermore, G. lamblia infection was detected in 15 (6.6%) of 228 faecal samples submitted to the laboratory for bacterial culture only. C. parvum/C. hominis infections were not diagnosed by routine procedures, but DNA from these organisms was detected in 4.3% of 950 DNA samples. A strong association with age was noted, with Cryptosporidium being detected in 21.8% of 110 children aged <5 years. C. hominis was the most prevalent species. E. histolytica was not detected in this study population. Analysis of microscopy data revealed that the number of additional parasites missed by PCR was small. Overall, the study demonstrated that a multiplex real-time PCR approach is a feasible diagnostic alternative in the clinical laboratory for the detection of parasitic infections in patients consulting GPs because of gastrointestinal symptoms. SN - 1198-743X UR - https://www.unboundmedicine.com/medline/citation/17714523/Detection_of_diarrhoea_causing_protozoa_in_general_practice_patients_in_The_Netherlands_by_multiplex_real_time_PCR_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)62088-7 DB - PRIME DP - Unbound Medicine ER -