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TRPV1-mediated diuresis and natriuresis induced by hypertonic saline perfusion of the renal pelvis.
Am J Nephrol. 2007; 27(5):530-7.AJ

Abstract

BACKGROUND

The transient receptor potential vanilloid type 1 (TRPV1) channel is known to be activated by multiple stimuli, albeit its role in mediating renal function is largely unknown. This study was designed to test the hypothesis that TRPV1 mediates diuresis and natriuresis induced by hypertonic saline perfusion into the pelvis.

METHODS

NaCl or KCl was perfused into the left renal pelvis of rats at a rate without changing renal pelvic pressure. Afferent renal nerve activity (ARNA), urine flow rate (V) and urinary sodium excretion (UNaV) in the presence or absence of selective antagonists of TRPV1, capsazepine (CAPZ), or neurokinin-1 (NK1) receptors, RP67580, were examined.

RESULTS

Unilateral renal pelvis perfusion of NaCl at 600 mM, but not 150 or 300 mM, increased ipsilateral ARNA and contralateral V and UNaV, which were blocked by ipsilateral administration of CAPZ or RP67580. In contrast, KCl perfused at 150 or 300 mM, but not 600 mM, increased ipsilateral ARNA and contralateral V and UNaV, which were insensitive to CAPZ.

CONCLUSION

Unilateral hypertonic saline perfusion causes contralateral diuresis and natriuresis via TRPV1 or NK1 activation, indicating that these receptors may play a critical role in sensing microenvironmental changes in the renal pelvis to modulate renal function in health and disease.

Authors+Show Affiliations

Department of Medicine, Michigan State University, East Lansing, Mich. 48823, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17717412

Citation

Zhu, Yi, et al. "TRPV1-mediated Diuresis and Natriuresis Induced By Hypertonic Saline Perfusion of the Renal Pelvis." American Journal of Nephrology, vol. 27, no. 5, 2007, pp. 530-7.
Zhu Y, Xie C, Wang DH. TRPV1-mediated diuresis and natriuresis induced by hypertonic saline perfusion of the renal pelvis. Am J Nephrol. 2007;27(5):530-7.
Zhu, Y., Xie, C., & Wang, D. H. (2007). TRPV1-mediated diuresis and natriuresis induced by hypertonic saline perfusion of the renal pelvis. American Journal of Nephrology, 27(5), 530-7.
Zhu Y, Xie C, Wang DH. TRPV1-mediated Diuresis and Natriuresis Induced By Hypertonic Saline Perfusion of the Renal Pelvis. Am J Nephrol. 2007;27(5):530-7. PubMed PMID: 17717412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPV1-mediated diuresis and natriuresis induced by hypertonic saline perfusion of the renal pelvis. AU - Zhu,Yi, AU - Xie,Chaoqin, AU - Wang,Donna H, Y1 - 2007/08/24/ PY - 2007/05/23/received PY - 2007/07/11/accepted PY - 2007/8/25/pubmed PY - 2007/10/17/medline PY - 2007/8/25/entrez SP - 530 EP - 7 JF - American journal of nephrology JO - Am J Nephrol VL - 27 IS - 5 N2 - BACKGROUND: The transient receptor potential vanilloid type 1 (TRPV1) channel is known to be activated by multiple stimuli, albeit its role in mediating renal function is largely unknown. This study was designed to test the hypothesis that TRPV1 mediates diuresis and natriuresis induced by hypertonic saline perfusion into the pelvis. METHODS: NaCl or KCl was perfused into the left renal pelvis of rats at a rate without changing renal pelvic pressure. Afferent renal nerve activity (ARNA), urine flow rate (V) and urinary sodium excretion (UNaV) in the presence or absence of selective antagonists of TRPV1, capsazepine (CAPZ), or neurokinin-1 (NK1) receptors, RP67580, were examined. RESULTS: Unilateral renal pelvis perfusion of NaCl at 600 mM, but not 150 or 300 mM, increased ipsilateral ARNA and contralateral V and UNaV, which were blocked by ipsilateral administration of CAPZ or RP67580. In contrast, KCl perfused at 150 or 300 mM, but not 600 mM, increased ipsilateral ARNA and contralateral V and UNaV, which were insensitive to CAPZ. CONCLUSION: Unilateral hypertonic saline perfusion causes contralateral diuresis and natriuresis via TRPV1 or NK1 activation, indicating that these receptors may play a critical role in sensing microenvironmental changes in the renal pelvis to modulate renal function in health and disease. SN - 1421-9670 UR - https://www.unboundmedicine.com/medline/citation/17717412/TRPV1_mediated_diuresis_and_natriuresis_induced_by_hypertonic_saline_perfusion_of_the_renal_pelvis_ L2 - https://www.karger.com?DOI=10.1159/000107665 DB - PRIME DP - Unbound Medicine ER -