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Antimicrobial activity of the methanolic extract and of the chemical constituents isolated from Newbouldia laevis.
Pharmazie. 2007 Jul; 62(7):552-6.P

Abstract

The methanolic extract (NLB) and ten compounds isolated from the root bark of Newbouldia laevis Seem, namely chrysoeriol (1), newbouldiaquinone (2), 2-acetylfuro-1,4-naphthoquinone (3), 2-hydroxy-3-methoxy-9,10-dioxo-9,10-dihydroanthracene-1-carbaldehyde (4), lapachol (5), beta-sitosterol-3-O-beta-D-glucopyranoside (6), oleanolic acid (7), canthic acid (8) newbouldiamide (9) and 2-(4-hydroxyphenyl)-ethyltrioctanoate (10), were tested for in vitro antimicrobial activity. Twenty one microorganisms belonging to six Gram-positive and twelve Gram-negative bacterial species as well as three yeasts from Candida species were tested for their susceptibility to NLB and the pure isolated compounds based on the Agar Hole Diffusion test and the Liquid Dilution method. The Hole Diffusion assay indicated that NLB and compound 7 were active against all tested pathogens while other compounds showed selective activity with the antimicrobial spectra varying from 76% (compound 10) to 95 % (compound 6). Minimal inhibitory concentrations (MIC) also illustrated the important antimicrobial activity of NLB and of the isolated compounds. MIC values obtained varied from 9.76 to 312.50 microg/ml for NLB, and 0.038 to 9.76 microg/ml for pure compounds against most of the tested microorganisms. The antimicrobial activities of compounds 2, 4 and 9 are described here extensively for the first time. The results indicate a promising basis for the use of Newbouldia laevis and some of its active principles in the treatment of infectious diseases.

Authors+Show Affiliations

Kuete V
Department of Biochemistry, Yaounde University I, Yaounde, Cameroon. kuetevictor@yahoo.fr
Eyong KO
No affiliation info available
Folefoc GN
No affiliation info available
Beng VP
No affiliation info available
Hussain H
No affiliation info available
Krohn K
No affiliation info available
Nkengfack AE
No affiliation info available

MeSH

Anti-Infective AgentsCulture MediaDiffusionGram-Negative BacteriaGram-Positive BacteriaMethanolMicrobial Sensitivity TestsPlant BarkPlant ExtractsPlantsSolventsYeasts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17718200

Citation

Kuete, V, et al. "Antimicrobial Activity of the Methanolic Extract and of the Chemical Constituents Isolated From Newbouldia Laevis." Die Pharmazie, vol. 62, no. 7, 2007, pp. 552-6.
Kuete V, Eyong KO, Folefoc GN, et al. Antimicrobial activity of the methanolic extract and of the chemical constituents isolated from Newbouldia laevis. Pharmazie. 2007;62(7):552-6.
Kuete, V., Eyong, K. O., Folefoc, G. N., Beng, V. P., Hussain, H., Krohn, K., & Nkengfack, A. E. (2007). Antimicrobial activity of the methanolic extract and of the chemical constituents isolated from Newbouldia laevis. Die Pharmazie, 62(7), 552-6.
Kuete V, et al. Antimicrobial Activity of the Methanolic Extract and of the Chemical Constituents Isolated From Newbouldia Laevis. Pharmazie. 2007;62(7):552-6. PubMed PMID: 17718200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial activity of the methanolic extract and of the chemical constituents isolated from Newbouldia laevis. AU - Kuete,V, AU - Eyong,K O, AU - Folefoc,G N, AU - Beng,V P, AU - Hussain,H, AU - Krohn,K, AU - Nkengfack,A E, PY - 2007/8/28/pubmed PY - 2007/9/22/medline PY - 2007/8/28/entrez SP - 552 EP - 6 JF - Die Pharmazie JO - Pharmazie VL - 62 IS - 7 N2 - The methanolic extract (NLB) and ten compounds isolated from the root bark of Newbouldia laevis Seem, namely chrysoeriol (1), newbouldiaquinone (2), 2-acetylfuro-1,4-naphthoquinone (3), 2-hydroxy-3-methoxy-9,10-dioxo-9,10-dihydroanthracene-1-carbaldehyde (4), lapachol (5), beta-sitosterol-3-O-beta-D-glucopyranoside (6), oleanolic acid (7), canthic acid (8) newbouldiamide (9) and 2-(4-hydroxyphenyl)-ethyltrioctanoate (10), were tested for in vitro antimicrobial activity. Twenty one microorganisms belonging to six Gram-positive and twelve Gram-negative bacterial species as well as three yeasts from Candida species were tested for their susceptibility to NLB and the pure isolated compounds based on the Agar Hole Diffusion test and the Liquid Dilution method. The Hole Diffusion assay indicated that NLB and compound 7 were active against all tested pathogens while other compounds showed selective activity with the antimicrobial spectra varying from 76% (compound 10) to 95 % (compound 6). Minimal inhibitory concentrations (MIC) also illustrated the important antimicrobial activity of NLB and of the isolated compounds. MIC values obtained varied from 9.76 to 312.50 microg/ml for NLB, and 0.038 to 9.76 microg/ml for pure compounds against most of the tested microorganisms. The antimicrobial activities of compounds 2, 4 and 9 are described here extensively for the first time. The results indicate a promising basis for the use of Newbouldia laevis and some of its active principles in the treatment of infectious diseases. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/17718200/Antimicrobial_activity_of_the_methanolic_extract_and_of_the_chemical_constituents_isolated_from_Newbouldia_laevis_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:17718200 DB - PRIME DP - Unbound Medicine ER -