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Antioxidant status and lipid peroxidation end products in patients of type 1 diabetes mellitus.
J Med Assoc Thai. 2006 Nov; 89 Suppl 5:S141-6.JM

Abstract

BACKGROUND AND OBJECTIVE

In Type 1 diabetes mellitus (DM), hyperglycemia is considered a primary cause of diabetic vascular complications and is associated with oxidative stress. The role of antioxidants, particularly alpha tocopherol, in Type 1 DM and its contribution in the development of vascular complications is not clear. Therefore, the present study aims to investigate the relationship between antioxidant status (alpha tocopherol) and lipid peroxidation end products (malondialdehyde; MDA) in the plasma of 20 Type 1 DM and 20 nondiabetic healthy control subjects.

MATERIAL AND METHOD

Lipid levels in all subjects were analyzed spectrophotometrically by enzymatic reagent kits. Plasma MDA was assessed by spectrofluorometry, whereas plasma alpha tocopherol was estimated by high performance liquid chromatography in Type 1 DM as well as in the control subjects of matched sex and ages. The results of Type 1 DM were compared with a control group using unpaired Student's t-test. The correlations between fasting plasma glucose and other laboratory parameters were assessed by Pearson rank correlation coefficient.

RESULTS

The plasma MDA concentration was significantly higher in Type 1 diabetic patients as compared to controls, (p < 0.01). A significantly reduced plasma antioxidant status of Type 1 DM patients was found only in alpha tocopherol / total lipid as compared to controls (p < 0.05). However, no significant difference was observed in plasma a tocopherol and a tocopherol / total cholesterol (p > 0.05) as compared to the control subjects. The positive correlation between MDA and FPG was demonstrated in Type 1 diabetic compared with normal subjects.

CONCLUSION

We conclude that antioxidant supplementation may be necessary for treatment to reduce oxidative stress for diabetic complication protection in Type 1 DM.

Authors+Show Affiliations

Peerapatdit T
Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Likidlilid A
No affiliation info available
Patchanans N
No affiliation info available
Somkasetrin A
No affiliation info available

MeSH

AdolescentAdultAntioxidantsArteriosclerosisBiomarkersBlood GlucoseCase-Control StudiesChromatography, High Pressure LiquidDiabetes Mellitus, Type 1FemaleHumansLipid PeroxidationMaleMiddle AgedOxidative StressRisk FactorsSpectrophotometryalpha-Tocopherol

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17718255

Citation

Peerapatdit, Thavatchai, et al. "Antioxidant Status and Lipid Peroxidation End Products in Patients of Type 1 Diabetes Mellitus." Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, vol. 89 Suppl 5, 2006, pp. S141-6.
Peerapatdit T, Likidlilid A, Patchanans N, et al. Antioxidant status and lipid peroxidation end products in patients of type 1 diabetes mellitus. J Med Assoc Thai. 2006;89 Suppl 5:S141-6.
Peerapatdit, T., Likidlilid, A., Patchanans, N., & Somkasetrin, A. (2006). Antioxidant status and lipid peroxidation end products in patients of type 1 diabetes mellitus. Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 89 Suppl 5, S141-6.
Peerapatdit T, et al. Antioxidant Status and Lipid Peroxidation End Products in Patients of Type 1 Diabetes Mellitus. J Med Assoc Thai. 2006;89 Suppl 5:S141-6. PubMed PMID: 17718255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant status and lipid peroxidation end products in patients of type 1 diabetes mellitus. AU - Peerapatdit,Thavatchai, AU - Likidlilid,Atip, AU - Patchanans,Natchai, AU - Somkasetrin,Anchaleekorn, PY - 2007/8/28/pubmed PY - 2007/10/17/medline PY - 2007/8/28/entrez SP - S141 EP - 6 JF - Journal of the Medical Association of Thailand = Chotmaihet thangphaet JO - J Med Assoc Thai VL - 89 Suppl 5 N2 - BACKGROUND AND OBJECTIVE: In Type 1 diabetes mellitus (DM), hyperglycemia is considered a primary cause of diabetic vascular complications and is associated with oxidative stress. The role of antioxidants, particularly alpha tocopherol, in Type 1 DM and its contribution in the development of vascular complications is not clear. Therefore, the present study aims to investigate the relationship between antioxidant status (alpha tocopherol) and lipid peroxidation end products (malondialdehyde; MDA) in the plasma of 20 Type 1 DM and 20 nondiabetic healthy control subjects. MATERIAL AND METHOD: Lipid levels in all subjects were analyzed spectrophotometrically by enzymatic reagent kits. Plasma MDA was assessed by spectrofluorometry, whereas plasma alpha tocopherol was estimated by high performance liquid chromatography in Type 1 DM as well as in the control subjects of matched sex and ages. The results of Type 1 DM were compared with a control group using unpaired Student's t-test. The correlations between fasting plasma glucose and other laboratory parameters were assessed by Pearson rank correlation coefficient. RESULTS: The plasma MDA concentration was significantly higher in Type 1 diabetic patients as compared to controls, (p < 0.01). A significantly reduced plasma antioxidant status of Type 1 DM patients was found only in alpha tocopherol / total lipid as compared to controls (p < 0.05). However, no significant difference was observed in plasma a tocopherol and a tocopherol / total cholesterol (p > 0.05) as compared to the control subjects. The positive correlation between MDA and FPG was demonstrated in Type 1 diabetic compared with normal subjects. CONCLUSION: We conclude that antioxidant supplementation may be necessary for treatment to reduce oxidative stress for diabetic complication protection in Type 1 DM. SN - 0125-2208 UR - https://www.unboundmedicine.com/medline/citation/17718255/Antioxidant_status_and_lipid_peroxidation_end_products_in_patients_of_type_1_diabetes_mellitus_ L2 - http://www.diseaseinfosearch.org/result/8214 DB - PRIME DP - Unbound Medicine ER -