Tags

Type your tag names separated by a space and hit enter

Large scale pesticide multiresidue methods in food combining liquid chromatography--time-of-flight mass spectrometry and tandem mass spectrometry.
Anal Chem. 2007 Oct 01; 79(19):7308-23.AC

Abstract

Liquid chromatography tandem mass spectrometry (LC-MS/MS) and liquid chromatography time-of-flight mass spectrometry (LC-TOFMS) are powerful and complementary techniques that can independently cover the majority of the challenges related with pesticide residue food control. The sequential combination of both systems benefits from their complementary advantages and assists to increase the performance and to simplify routine large scale pesticide multiresidue methods. The proposed approach consists of three stages: (1) automated pesticide screening by LC-TOFMS; (2) identification by LC-TOFMS accurate mass measurements; and (3) confirmation and quantitation by LC-MS/MS. We have developed a fast comprehensive (identification/confirmation + quantitation) automated screening method for 100 target pesticides in crops. In the first stage, a set of data including m/z accurate mass windows (within 20 mDa width) and retention time is obtained (using a standard solution containing all the targeted pesticides) in order to build the automated screening procedure, which is created automatically by assigning retention time and the m/z mass window for each target pesticide. Samples are then analyzed, and the method enables the screening and preliminary identification of the species first by retention time and m/z mass window, followed by subsequent identification (only if positive results) by LC-TOFMS accurate mass measurements. After that, final confirmation of the positive findings using two MRM transitions and accurate quantitation is performed by LC-MS/MS using a hybrid triple quadrupole linear ion trap (QqLIT) mass spectrometer. In addition, the use of this QqLIT instrument also offers additional advantageous scanning modes (enhanced product ion and MS3 modes) for confirmatory purposes in compounds with poor fragmentation. Examples of applications to real samples show the potential of the proposed approach, including the detection of nonselected "a priori" compounds as a typical case of retrospective evaluation of banned or misused substances.

Authors+Show Affiliations

Department of Physical and Analytical Chemistry, University of Jaén, 23071 Jaén, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17718536

Citation

García-Reyes, Juan F., et al. "Large Scale Pesticide Multiresidue Methods in Food Combining Liquid Chromatography--time-of-flight Mass Spectrometry and Tandem Mass Spectrometry." Analytical Chemistry, vol. 79, no. 19, 2007, pp. 7308-23.
García-Reyes JF, Hernando MD, Ferrer C, et al. Large scale pesticide multiresidue methods in food combining liquid chromatography--time-of-flight mass spectrometry and tandem mass spectrometry. Anal Chem. 2007;79(19):7308-23.
García-Reyes, J. F., Hernando, M. D., Ferrer, C., Molina-Díaz, A., & Fernández-Alba, A. R. (2007). Large scale pesticide multiresidue methods in food combining liquid chromatography--time-of-flight mass spectrometry and tandem mass spectrometry. Analytical Chemistry, 79(19), 7308-23.
García-Reyes JF, et al. Large Scale Pesticide Multiresidue Methods in Food Combining Liquid Chromatography--time-of-flight Mass Spectrometry and Tandem Mass Spectrometry. Anal Chem. 2007 Oct 1;79(19):7308-23. PubMed PMID: 17718536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large scale pesticide multiresidue methods in food combining liquid chromatography--time-of-flight mass spectrometry and tandem mass spectrometry. AU - García-Reyes,Juan F, AU - Hernando,M Dolores, AU - Ferrer,Carmen, AU - Molina-Díaz,Antonio, AU - Fernández-Alba,Amadeo R, Y1 - 2007/08/25/ PY - 2007/8/28/pubmed PY - 2008/1/8/medline PY - 2007/8/28/entrez SP - 7308 EP - 23 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 19 N2 - Liquid chromatography tandem mass spectrometry (LC-MS/MS) and liquid chromatography time-of-flight mass spectrometry (LC-TOFMS) are powerful and complementary techniques that can independently cover the majority of the challenges related with pesticide residue food control. The sequential combination of both systems benefits from their complementary advantages and assists to increase the performance and to simplify routine large scale pesticide multiresidue methods. The proposed approach consists of three stages: (1) automated pesticide screening by LC-TOFMS; (2) identification by LC-TOFMS accurate mass measurements; and (3) confirmation and quantitation by LC-MS/MS. We have developed a fast comprehensive (identification/confirmation + quantitation) automated screening method for 100 target pesticides in crops. In the first stage, a set of data including m/z accurate mass windows (within 20 mDa width) and retention time is obtained (using a standard solution containing all the targeted pesticides) in order to build the automated screening procedure, which is created automatically by assigning retention time and the m/z mass window for each target pesticide. Samples are then analyzed, and the method enables the screening and preliminary identification of the species first by retention time and m/z mass window, followed by subsequent identification (only if positive results) by LC-TOFMS accurate mass measurements. After that, final confirmation of the positive findings using two MRM transitions and accurate quantitation is performed by LC-MS/MS using a hybrid triple quadrupole linear ion trap (QqLIT) mass spectrometer. In addition, the use of this QqLIT instrument also offers additional advantageous scanning modes (enhanced product ion and MS3 modes) for confirmatory purposes in compounds with poor fragmentation. Examples of applications to real samples show the potential of the proposed approach, including the detection of nonselected "a priori" compounds as a typical case of retrospective evaluation of banned or misused substances. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17718536/Large_scale_pesticide_multiresidue_methods_in_food_combining_liquid_chromatography__time_of_flight_mass_spectrometry_and_tandem_mass_spectrometry_ L2 - https://doi.org/10.1021/ac070855v DB - PRIME DP - Unbound Medicine ER -