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Highly sensitive and selective spectrophotometric and spectrofluorimetric methods for the determination of ropinirole hydrochloride in tablets.
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Jun; 70(1):69-78.SA

Abstract

Three sensitive, selective, accurate spectrophotometric and spectrofluorimetric methods have been developed for the determination of ropinirole hydrochloride in tablets. The first method was based on measuring the absorbance of drug solution in methanol at 250 nm. The Beer's law was obeyed in the concentration range 2.5-24 microg ml(-1). The second method was based on the charge transfer reaction of drug, as n-electron donor with 7,7,8,8-tetracyanoquinodimethane (TCNQ), as pi-acceptor in acetonitrile to give radical anions that are measured at 842 nm. The Beer's law was obeyed in the concentration range 0.6-8 microg ml(-1). The third method was based on derivatization reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in borate buffer of pH 8.5 followed by measuring the fluorescence intensity at 525 nm with excitation at 464 nm in chloroform. Beer's law was obeyed in the concentration range 0.01-1.3 microg ml(-1). The derivatization reaction product of drug with NBD-Cl was characterized by IR, 1H NMR and mass spectroscopy. The developed methods were validated. The following analytical parameters were investigated: the molar absorptivity (epsilon), limit of detection (LOD, microg ml(-1)) and limit of quantitation (LOQ, microg ml(-1)), precision, accuracy, recovery, and Sandell's sensitivity. Selectivity was validated by subjecting stock solution of ropinirole to acidic, basic, oxidative, and thermal degradation. No interference was observed from common excipients present in formulations. The proposed methods were successfully applied for determination of drug in tablets. The results of these proposed methods were compared with each other statistically.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Beyazit, Istanbul, Turkey. aydogmus@istanbul.edu.tr

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17719838

Citation

Aydoğmuş, Zeynep. "Highly Sensitive and Selective Spectrophotometric and Spectrofluorimetric Methods for the Determination of Ropinirole Hydrochloride in Tablets." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 70, no. 1, 2008, pp. 69-78.
Aydoğmuş Z. Highly sensitive and selective spectrophotometric and spectrofluorimetric methods for the determination of ropinirole hydrochloride in tablets. Spectrochim Acta A Mol Biomol Spectrosc. 2008;70(1):69-78.
Aydoğmuş, Z. (2008). Highly sensitive and selective spectrophotometric and spectrofluorimetric methods for the determination of ropinirole hydrochloride in tablets. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 70(1), 69-78.
Aydoğmuş Z. Highly Sensitive and Selective Spectrophotometric and Spectrofluorimetric Methods for the Determination of Ropinirole Hydrochloride in Tablets. Spectrochim Acta A Mol Biomol Spectrosc. 2008;70(1):69-78. PubMed PMID: 17719838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive and selective spectrophotometric and spectrofluorimetric methods for the determination of ropinirole hydrochloride in tablets. A1 - Aydoğmuş,Zeynep, Y1 - 2007/07/17/ PY - 2007/03/23/received PY - 2007/06/15/revised PY - 2007/07/12/accepted PY - 2007/8/28/pubmed PY - 2008/8/6/medline PY - 2007/8/28/entrez SP - 69 EP - 78 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 70 IS - 1 N2 - Three sensitive, selective, accurate spectrophotometric and spectrofluorimetric methods have been developed for the determination of ropinirole hydrochloride in tablets. The first method was based on measuring the absorbance of drug solution in methanol at 250 nm. The Beer's law was obeyed in the concentration range 2.5-24 microg ml(-1). The second method was based on the charge transfer reaction of drug, as n-electron donor with 7,7,8,8-tetracyanoquinodimethane (TCNQ), as pi-acceptor in acetonitrile to give radical anions that are measured at 842 nm. The Beer's law was obeyed in the concentration range 0.6-8 microg ml(-1). The third method was based on derivatization reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in borate buffer of pH 8.5 followed by measuring the fluorescence intensity at 525 nm with excitation at 464 nm in chloroform. Beer's law was obeyed in the concentration range 0.01-1.3 microg ml(-1). The derivatization reaction product of drug with NBD-Cl was characterized by IR, 1H NMR and mass spectroscopy. The developed methods were validated. The following analytical parameters were investigated: the molar absorptivity (epsilon), limit of detection (LOD, microg ml(-1)) and limit of quantitation (LOQ, microg ml(-1)), precision, accuracy, recovery, and Sandell's sensitivity. Selectivity was validated by subjecting stock solution of ropinirole to acidic, basic, oxidative, and thermal degradation. No interference was observed from common excipients present in formulations. The proposed methods were successfully applied for determination of drug in tablets. The results of these proposed methods were compared with each other statistically. SN - 1386-1425 UR - https://www.unboundmedicine.com/medline/citation/17719838/Highly_sensitive_and_selective_spectrophotometric_and_spectrofluorimetric_methods_for_the_determination_of_ropinirole_hydrochloride_in_tablets_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(07)00392-7 DB - PRIME DP - Unbound Medicine ER -