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[The inoculative properties of cold-adapted reassortant A(H5N2) influenza strain during intranasal administration to mice].
Vopr Virusol. 2007 Jul-Aug; 52(4):27-30.VV

Abstract

Classical genetic reassortant techniques were used to have a cold-adapted (ca) reassortant A/17/Duck/Potsdam/86/92 (H5N2) that inherited the hemagglutinin (HA) gene from the nonpathogenic avian virus A/Duck/Potsdam/ 1402-6186 (H5N2) and the genes of neuraminidase (NA) and non-glycated proteins from the ca attenuation donor A/Leningrad/134/17/57 (H2N2). All experiments were performed under increased biological protection (BSV-3+). The reassortant and parent H5N2 virus were non-pathogenic to Balb/c mice, the reassortant replication in the murine nasal passages (3.5 Ig EID50/ml) being higher than that in the lung (2.1 lg EID50/ml). Intranasal inoculation of mice with reassortant A/17/Duck/Potsdam/86/92 caused an immune response to both homological H5N2 virus and antigenically differing variants of influenza A (H5N1) virus isolated from humans in 1997 and 2003. The mice intranasally immunized with the ca reassortant were protected against fatal infection with the highly pathogenic A/Hong Kong/483/9797 (H5N1) virus and against infection with A/Hong Kong/213/03(H5N1) virus (80 and 100%, respectively).

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

17722607

Citation

Desheva, Iu A., et al. "[The Inoculative Properties of Cold-adapted Reassortant A(H5N2) Influenza Strain During Intranasal Administration to Mice]." Voprosy Virusologii, vol. 52, no. 4, 2007, pp. 27-30.
Desheva IuA, Lu Kh, Rekstin AR, et al. [The inoculative properties of cold-adapted reassortant A(H5N2) influenza strain during intranasal administration to mice]. Vopr Virusol. 2007;52(4):27-30.
Desheva, I. u. A., Lu, K. h., Rekstin, A. R., Kats, D. M., Rudenko, L. G., & Klimov, A. I. (2007). [The inoculative properties of cold-adapted reassortant A(H5N2) influenza strain during intranasal administration to mice]. Voprosy Virusologii, 52(4), 27-30.
Desheva IuA, et al. [The Inoculative Properties of Cold-adapted Reassortant A(H5N2) Influenza Strain During Intranasal Administration to Mice]. Vopr Virusol. 2007 Jul-Aug;52(4):27-30. PubMed PMID: 17722607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The inoculative properties of cold-adapted reassortant A(H5N2) influenza strain during intranasal administration to mice]. AU - Desheva,Iu A, AU - Lu,Kh, AU - Rekstin,A R, AU - Kats,D M, AU - Rudenko,L G, AU - Klimov,A I, PY - 2007/8/29/pubmed PY - 2007/10/6/medline PY - 2007/8/29/entrez SP - 27 EP - 30 JF - Voprosy virusologii JO - Vopr Virusol VL - 52 IS - 4 N2 - Classical genetic reassortant techniques were used to have a cold-adapted (ca) reassortant A/17/Duck/Potsdam/86/92 (H5N2) that inherited the hemagglutinin (HA) gene from the nonpathogenic avian virus A/Duck/Potsdam/ 1402-6186 (H5N2) and the genes of neuraminidase (NA) and non-glycated proteins from the ca attenuation donor A/Leningrad/134/17/57 (H2N2). All experiments were performed under increased biological protection (BSV-3+). The reassortant and parent H5N2 virus were non-pathogenic to Balb/c mice, the reassortant replication in the murine nasal passages (3.5 Ig EID50/ml) being higher than that in the lung (2.1 lg EID50/ml). Intranasal inoculation of mice with reassortant A/17/Duck/Potsdam/86/92 caused an immune response to both homological H5N2 virus and antigenically differing variants of influenza A (H5N1) virus isolated from humans in 1997 and 2003. The mice intranasally immunized with the ca reassortant were protected against fatal infection with the highly pathogenic A/Hong Kong/483/9797 (H5N1) virus and against infection with A/Hong Kong/213/03(H5N1) virus (80 and 100%, respectively). SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/17722607/[The_inoculative_properties_of_cold_adapted_reassortant_A_H5N2__influenza_strain_during_intranasal_administration_to_mice]_ L2 - https://medlineplus.gov/vaccines.html DB - PRIME DP - Unbound Medicine ER -