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High-performance liquid chromatographic determination of biogenic amines in wines with an experimental design optimization procedure.
Anal Chim Acta. 2006 Aug 04; 575(1):97-105.AC

Abstract

A novel and sensitive HPLC method for determining biogenic amines in wine samples is described. It involves pre-column labeling of the analytes with 1,2-naphthoquinone-4-sulfonate (NQS) and liquid-liquid extraction of derivatives with chloroform for analyte preconcentration and sample clean-up. A linear gradient elution consisting of a mixture of 2% of acetic acid aqueous solution and methanol is used to separate the amine derivatives in a C18 column. The eluted compounds are detected spectrophotometrically at 270 nm. The optimization of both derivatization and separation conditions is accomplished by means of factorial and central composite designs and multicriteria decision functions. The analytical parameters of the method are established using red wine samples. Detection limits range from 0.006 to 0.315 mg L(-1). The run-to-run repeatabilities of retention times and peak areas are around 0.6 and 5.6%, respectively. Recoveries ranging from 91.9 to 105% prove the accuracy of the method for determining histamine, putrescine, cadaverine, tryptamine, phenylethylamine and serotonin in red wines. The proposed method has been applied to the analysis of commercial wines from different Spanish regions.

Authors+Show Affiliations

Department of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028 Barcelona, Spain. nati@apolo.qui.ub.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17723577

Citation

García-Villar, Natividad, et al. "High-performance Liquid Chromatographic Determination of Biogenic Amines in Wines With an Experimental Design Optimization Procedure." Analytica Chimica Acta, vol. 575, no. 1, 2006, pp. 97-105.
García-Villar N, Saurina J, Hernández-Cassou S. High-performance liquid chromatographic determination of biogenic amines in wines with an experimental design optimization procedure. Anal Chim Acta. 2006;575(1):97-105.
García-Villar, N., Saurina, J., & Hernández-Cassou, S. (2006). High-performance liquid chromatographic determination of biogenic amines in wines with an experimental design optimization procedure. Analytica Chimica Acta, 575(1), 97-105.
García-Villar N, Saurina J, Hernández-Cassou S. High-performance Liquid Chromatographic Determination of Biogenic Amines in Wines With an Experimental Design Optimization Procedure. Anal Chim Acta. 2006 Aug 4;575(1):97-105. PubMed PMID: 17723577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatographic determination of biogenic amines in wines with an experimental design optimization procedure. AU - García-Villar,Natividad, AU - Saurina,Javier, AU - Hernández-Cassou,Santiago, Y1 - 2006/05/27/ PY - 2006/01/31/received PY - 2006/05/12/revised PY - 2006/05/18/accepted PY - 2007/8/29/pubmed PY - 2007/8/29/medline PY - 2007/8/29/entrez SP - 97 EP - 105 JF - Analytica chimica acta JO - Anal Chim Acta VL - 575 IS - 1 N2 - A novel and sensitive HPLC method for determining biogenic amines in wine samples is described. It involves pre-column labeling of the analytes with 1,2-naphthoquinone-4-sulfonate (NQS) and liquid-liquid extraction of derivatives with chloroform for analyte preconcentration and sample clean-up. A linear gradient elution consisting of a mixture of 2% of acetic acid aqueous solution and methanol is used to separate the amine derivatives in a C18 column. The eluted compounds are detected spectrophotometrically at 270 nm. The optimization of both derivatization and separation conditions is accomplished by means of factorial and central composite designs and multicriteria decision functions. The analytical parameters of the method are established using red wine samples. Detection limits range from 0.006 to 0.315 mg L(-1). The run-to-run repeatabilities of retention times and peak areas are around 0.6 and 5.6%, respectively. Recoveries ranging from 91.9 to 105% prove the accuracy of the method for determining histamine, putrescine, cadaverine, tryptamine, phenylethylamine and serotonin in red wines. The proposed method has been applied to the analysis of commercial wines from different Spanish regions. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17723577/High_performance_liquid_chromatographic_determination_of_biogenic_amines_in_wines_with_an_experimental_design_optimization_procedure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(06)01141-X DB - PRIME DP - Unbound Medicine ER -
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