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Y box-binding protein-1 binds to the dengue virus 3'-untranslated region and mediates antiviral effects.
J Biol Chem. 2007 Oct 19; 282(42):30497-508.JB

Abstract

Dengue virus, a member of the family Flaviviridae, poses a serious public health threat worldwide. Dengue virus is a positive-sense RNA virus that harbors a genome of approximately 10.7 kb. Replication of dengue virus is mediated coordinately by cis-acting genomic sequences, viral proteins, and host cell factors. We have isolated and identified several host cell factors from baby hamster kidney cell extracts that bind with high specificity and high affinity to sequences within the untranslated regions of the dengue virus genome. Among the factors identified, Y box-binding protein-1 (YB-1) and the heterogeneous nuclear ribonucleoproteins (hnRNPs), hnRNP A1, hnRNP A2/B1, and hnRNP Q, bind to the dengue virus 3'-untranslated region. Further analysis indicated that YB-1 binds to the dengue virus 3' stem loop, a conserved structural feature located at the 3' terminus of the 3'-untranslated region of many flaviviruses. Analysis of the impact of YB-1 on replication of dengue virus in YB-1+/+ and YB-1-/- mouse embryo fibroblasts indicated that host YB-1 mediates an antiviral effect. Further studies demonstrated that this antiviral impact is due, at least in part, to a repressive role of YB-1 on dengue virus translation via a mechanism that requires viral genomic sequences. These results suggest a novel role for YB-1 as an antiviral host cell factor.

Authors+Show Affiliations

Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720-7360, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17726010

Citation

Paranjape, Suman Marie, and Eva Harris. "Y Box-binding Protein-1 Binds to the Dengue Virus 3'-untranslated Region and Mediates Antiviral Effects." The Journal of Biological Chemistry, vol. 282, no. 42, 2007, pp. 30497-508.
Paranjape SM, Harris E. Y box-binding protein-1 binds to the dengue virus 3'-untranslated region and mediates antiviral effects. J Biol Chem. 2007;282(42):30497-508.
Paranjape, S. M., & Harris, E. (2007). Y box-binding protein-1 binds to the dengue virus 3'-untranslated region and mediates antiviral effects. The Journal of Biological Chemistry, 282(42), 30497-508.
Paranjape SM, Harris E. Y Box-binding Protein-1 Binds to the Dengue Virus 3'-untranslated Region and Mediates Antiviral Effects. J Biol Chem. 2007 Oct 19;282(42):30497-508. PubMed PMID: 17726010.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Y box-binding protein-1 binds to the dengue virus 3'-untranslated region and mediates antiviral effects. AU - Paranjape,Suman Marie, AU - Harris,Eva, Y1 - 2007/08/28/ PY - 2007/8/30/pubmed PY - 2007/12/13/medline PY - 2007/8/30/entrez SP - 30497 EP - 508 JF - The Journal of biological chemistry JO - J Biol Chem VL - 282 IS - 42 N2 - Dengue virus, a member of the family Flaviviridae, poses a serious public health threat worldwide. Dengue virus is a positive-sense RNA virus that harbors a genome of approximately 10.7 kb. Replication of dengue virus is mediated coordinately by cis-acting genomic sequences, viral proteins, and host cell factors. We have isolated and identified several host cell factors from baby hamster kidney cell extracts that bind with high specificity and high affinity to sequences within the untranslated regions of the dengue virus genome. Among the factors identified, Y box-binding protein-1 (YB-1) and the heterogeneous nuclear ribonucleoproteins (hnRNPs), hnRNP A1, hnRNP A2/B1, and hnRNP Q, bind to the dengue virus 3'-untranslated region. Further analysis indicated that YB-1 binds to the dengue virus 3' stem loop, a conserved structural feature located at the 3' terminus of the 3'-untranslated region of many flaviviruses. Analysis of the impact of YB-1 on replication of dengue virus in YB-1+/+ and YB-1-/- mouse embryo fibroblasts indicated that host YB-1 mediates an antiviral effect. Further studies demonstrated that this antiviral impact is due, at least in part, to a repressive role of YB-1 on dengue virus translation via a mechanism that requires viral genomic sequences. These results suggest a novel role for YB-1 as an antiviral host cell factor. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/17726010/Y_box_binding_protein_1_binds_to_the_dengue_virus_3'_untranslated_region_and_mediates_antiviral_effects_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)59826-8 DB - PRIME DP - Unbound Medicine ER -