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Measurement of cotinine in urine by liquid chromatography tandem mass spectrometry.
Ann Clin Biochem. 2007 Sep; 44(Pt 5):455-62.AC

Abstract

BACKGROUND

Cotinine is the major metabolite of nicotine. It is also a specific biomarker for nicotine exposure in cigarette smokers. The measurement of urine cotinine concentration will enable: (1) the assessment of the smoking status of lung transplant patients and (2) tobacco abstinence to be studied in patients during treatment under smoking cessation programmes.

METHODS

We have developed and validated a method for the measurement of urinary cotinine using reversed phase high-performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (LC-MS/MS). This technique utilizes online ion exchange coupled with an analytical column to eliminate ion suppression effects. The chromatography was performed using a Waters 2795 Alliance HT LC system.

RESULTS

Cotinine and d3-cotinine had a retention time of 2.5 min and the cycle time from injection to injection was 4 min. The transition identified for cotinine was m/z 177.1>79.6 and for d3-cotinine m/z 180.2>79.6. This method was linear up to 1000 microg/L. Mean recovery of the assay was 112% with a range of 107-117% (n=9). The limit of quantitation for this assay was 2.5 microg/L and the limit of detection was 0.156 microg/L. The intra- and inter-assay imprecision was <12% and <10% respectively over a concentration range of 22-660 microg/L.

CONCLUSIONS

We have developed a robust and rapid assay for measuring and analysing urine cotinine by LC-MS/MS, by utilizing a technique, which has reduced ion suppression effects. Ultimately, the method will facilitate the assessment of lung transplant patients' smoking status.

Authors+Show Affiliations

Department of Clinical Chemistry, Royal Liverpool University Hospital, Prescot Street, Liverpool L7 8XP, UK. carieannchadwick@yahoo.co.ukNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17761031

Citation

Chadwick, Carrie Ann, and Brian Keevil. "Measurement of Cotinine in Urine By Liquid Chromatography Tandem Mass Spectrometry." Annals of Clinical Biochemistry, vol. 44, no. Pt 5, 2007, pp. 455-62.
Chadwick CA, Keevil B. Measurement of cotinine in urine by liquid chromatography tandem mass spectrometry. Ann Clin Biochem. 2007;44(Pt 5):455-62.
Chadwick, C. A., & Keevil, B. (2007). Measurement of cotinine in urine by liquid chromatography tandem mass spectrometry. Annals of Clinical Biochemistry, 44(Pt 5), 455-62.
Chadwick CA, Keevil B. Measurement of Cotinine in Urine By Liquid Chromatography Tandem Mass Spectrometry. Ann Clin Biochem. 2007;44(Pt 5):455-62. PubMed PMID: 17761031.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of cotinine in urine by liquid chromatography tandem mass spectrometry. AU - Chadwick,Carrie Ann, AU - Keevil,Brian, PY - 2007/9/1/pubmed PY - 2007/12/21/medline PY - 2007/9/1/entrez SP - 455 EP - 62 JF - Annals of clinical biochemistry JO - Ann Clin Biochem VL - 44 IS - Pt 5 N2 - BACKGROUND: Cotinine is the major metabolite of nicotine. It is also a specific biomarker for nicotine exposure in cigarette smokers. The measurement of urine cotinine concentration will enable: (1) the assessment of the smoking status of lung transplant patients and (2) tobacco abstinence to be studied in patients during treatment under smoking cessation programmes. METHODS: We have developed and validated a method for the measurement of urinary cotinine using reversed phase high-performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (LC-MS/MS). This technique utilizes online ion exchange coupled with an analytical column to eliminate ion suppression effects. The chromatography was performed using a Waters 2795 Alliance HT LC system. RESULTS: Cotinine and d3-cotinine had a retention time of 2.5 min and the cycle time from injection to injection was 4 min. The transition identified for cotinine was m/z 177.1>79.6 and for d3-cotinine m/z 180.2>79.6. This method was linear up to 1000 microg/L. Mean recovery of the assay was 112% with a range of 107-117% (n=9). The limit of quantitation for this assay was 2.5 microg/L and the limit of detection was 0.156 microg/L. The intra- and inter-assay imprecision was <12% and <10% respectively over a concentration range of 22-660 microg/L. CONCLUSIONS: We have developed a robust and rapid assay for measuring and analysing urine cotinine by LC-MS/MS, by utilizing a technique, which has reduced ion suppression effects. Ultimately, the method will facilitate the assessment of lung transplant patients' smoking status. SN - 0004-5632 UR - https://www.unboundmedicine.com/medline/citation/17761031/Measurement_of_cotinine_in_urine_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://journals.sagepub.com/doi/10.1258/000456307781645996?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -