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Vasopressin response and shunting modulation in cirrhotic rats by chronic nitric oxide inhibition.
J Gastroenterol Hepatol. 2008 Jul; 23(7 Pt 2):e265-9.JG

Abstract

BACKGROUND AND AIM

Nitric oxide (NO) plays a significant role in the vascular hyposensitivity to vasoconstrictors in cirrhosis. Chronic NO inhibition improves the portal-systemic collateral responsiveness to arginine(8)-vasopressin (AVP) and ameliorates shunting degree in rats with prehepatic portal hypertension. This study investigated whether long-term NO inhibition by N(G)-nitro-L-arginine methyl ester (L-NAME) enhances the collateral vascular responsiveness to AVP and alleviates the severity of shunting in cirrhotic rats.

METHODS

Bile duct-ligated (BDL) rats received L-NAME in tap water (25 mg/kg/day) or tap water only (control) for 1 week from the 36th day after BDL. On the 43rd day, the mean arterial pressure and portal pressure were measured. With an in situ perfusion model of portal-systemic collateral vasculature, different concentrations of AVP (10(-10)-10(-7) mol/L) with a constant flow rate (12 mL/min) were applied to assess the perfusion pressure changes of collaterals. In addition, flow pressure curves were obtained with different flow rates (6-18 mL/min): the slopes serve as indices of collateral vascular resistance and the higher resistance indicates less collateral.

RESULTS

The mean arterial pressure was significantly increased after L-NAME treatment (P < 0.05), whereas the heart rate and portal pressure were not significantly modified. As compared with the controls, the L-NAME group exerted significantly higher perfusion pressure changes to AVP at the concentrations of 3 x 10(-8), 10(-7) and 3 x 10(-7) mol/L. In addition, chronic L-NAME administration induced collateral vascular resistance elevation, suggesting the attenuation of portal-systemic shunting.

CONCLUSION

Chronic NO inhibition improves the collateral vascular responsiveness to AVP and ameliorates portal-systemic shunting in BDL cirrhotic rats.

Authors+Show Affiliations

Department of Medicine, Division of Gastroenterology, Taipei Veterans General Hospital, and National Yang-Ming University School of Medicine, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17764528

Citation

Huang, Hui-Chun, et al. "Vasopressin Response and Shunting Modulation in Cirrhotic Rats By Chronic Nitric Oxide Inhibition." Journal of Gastroenterology and Hepatology, vol. 23, no. 7 Pt 2, 2008, pp. e265-9.
Huang HC, Wang SS, Lee FY, et al. Vasopressin response and shunting modulation in cirrhotic rats by chronic nitric oxide inhibition. J Gastroenterol Hepatol. 2008;23(7 Pt 2):e265-9.
Huang, H. C., Wang, S. S., Lee, F. Y., Chang, C. C., Chang, F. Y., Lin, H. C., Hou, M. C., & Lee, S. D. (2008). Vasopressin response and shunting modulation in cirrhotic rats by chronic nitric oxide inhibition. Journal of Gastroenterology and Hepatology, 23(7 Pt 2), e265-9.
Huang HC, et al. Vasopressin Response and Shunting Modulation in Cirrhotic Rats By Chronic Nitric Oxide Inhibition. J Gastroenterol Hepatol. 2008;23(7 Pt 2):e265-9. PubMed PMID: 17764528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vasopressin response and shunting modulation in cirrhotic rats by chronic nitric oxide inhibition. AU - Huang,Hui-Chun, AU - Wang,Sun-Sang, AU - Lee,Fa-Yauh, AU - Chang,Ching-Chih, AU - Chang,Full-Young, AU - Lin,Han-Chieh, AU - Hou,Ming-Chih, AU - Lee,Shou-Dong, Y1 - 2007/08/30/ PY - 2007/9/4/pubmed PY - 2008/10/31/medline PY - 2007/9/4/entrez SP - e265 EP - 9 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 23 IS - 7 Pt 2 N2 - BACKGROUND AND AIM: Nitric oxide (NO) plays a significant role in the vascular hyposensitivity to vasoconstrictors in cirrhosis. Chronic NO inhibition improves the portal-systemic collateral responsiveness to arginine(8)-vasopressin (AVP) and ameliorates shunting degree in rats with prehepatic portal hypertension. This study investigated whether long-term NO inhibition by N(G)-nitro-L-arginine methyl ester (L-NAME) enhances the collateral vascular responsiveness to AVP and alleviates the severity of shunting in cirrhotic rats. METHODS: Bile duct-ligated (BDL) rats received L-NAME in tap water (25 mg/kg/day) or tap water only (control) for 1 week from the 36th day after BDL. On the 43rd day, the mean arterial pressure and portal pressure were measured. With an in situ perfusion model of portal-systemic collateral vasculature, different concentrations of AVP (10(-10)-10(-7) mol/L) with a constant flow rate (12 mL/min) were applied to assess the perfusion pressure changes of collaterals. In addition, flow pressure curves were obtained with different flow rates (6-18 mL/min): the slopes serve as indices of collateral vascular resistance and the higher resistance indicates less collateral. RESULTS: The mean arterial pressure was significantly increased after L-NAME treatment (P < 0.05), whereas the heart rate and portal pressure were not significantly modified. As compared with the controls, the L-NAME group exerted significantly higher perfusion pressure changes to AVP at the concentrations of 3 x 10(-8), 10(-7) and 3 x 10(-7) mol/L. In addition, chronic L-NAME administration induced collateral vascular resistance elevation, suggesting the attenuation of portal-systemic shunting. CONCLUSION: Chronic NO inhibition improves the collateral vascular responsiveness to AVP and ameliorates portal-systemic shunting in BDL cirrhotic rats. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/17764528/Vasopressin_response_and_shunting_modulation_in_cirrhotic_rats_by_chronic_nitric_oxide_inhibition_ L2 - https://doi.org/10.1111/j.1440-1746.2007.05122.x DB - PRIME DP - Unbound Medicine ER -