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Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode.
Biosens Bioelectron. 2007 Nov 30; 23(4):528-35.BB

Abstract

A bienzymatic glucose biosensor was proposed for selective and sensitive detection of glucose. This mediatorless biosensor was made by simultaneous immobilization of glucose oxidase (GOD) and horseradish peroxidase (HRP) in an electropolymerized pyrrole (PPy) film on a single-wall carbon nanotubes (SWNT) coated electrode. The amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.1 versus Ag/AgCl to reduce the enzymatically produced H(2)O(2) with minimal interference from the coexisting electroactive compounds. The single-wall carbon nanotubes, sandwiched between the enzyme loading polypyrrole (PPy) layer and the conducting substrate (gold electrode), could efficiently promote the direct electron transfer of HRP. Operational characteristics of the bienzymatic sensor, in terms of linear range, detection limit, sensitivity, selectivity and stability, were presented in detail.

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Authors+Show Affiliations

Zhu L
School of Chemistry, Northeast Normal University, Changchun, 130024 Jilin, China. zhuliande@yahoo.com.cn <zhuliande@yahoo.com.cn>
Yang R
No affiliation info available
Zhai J
No affiliation info available
Tian C
No affiliation info available

MeSH

Biosensing TechniquesEnzymes, ImmobilizedGlucoseGlucose OxidaseHorseradish PeroxidaseMicroscopy, Electron, ScanningNanotubes, Carbon

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17764922

Citation

Zhu, Liande, et al. "Bienzymatic Glucose Biosensor Based On Co-immobilization of Peroxidase and Glucose Oxidase On a Carbon Nanotubes Electrode." Biosensors & Bioelectronics, vol. 23, no. 4, 2007, pp. 528-35.
Zhu L, Yang R, Zhai J, et al. Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode. Biosens Bioelectron. 2007;23(4):528-35.
Zhu, L., Yang, R., Zhai, J., & Tian, C. (2007). Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode. Biosensors & Bioelectronics, 23(4), 528-35.
Zhu L, et al. Bienzymatic Glucose Biosensor Based On Co-immobilization of Peroxidase and Glucose Oxidase On a Carbon Nanotubes Electrode. Biosens Bioelectron. 2007 Nov 30;23(4):528-35. PubMed PMID: 17764922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode. AU - Zhu,Liande, AU - Yang,Ruilan, AU - Zhai,Jiangli, AU - Tian,Chunyuan, Y1 - 2007/07/20/ PY - 2007/04/02/received PY - 2007/07/06/revised PY - 2007/07/11/accepted PY - 2007/9/4/pubmed PY - 2008/2/27/medline PY - 2007/9/4/entrez SP - 528 EP - 35 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 4 N2 - A bienzymatic glucose biosensor was proposed for selective and sensitive detection of glucose. This mediatorless biosensor was made by simultaneous immobilization of glucose oxidase (GOD) and horseradish peroxidase (HRP) in an electropolymerized pyrrole (PPy) film on a single-wall carbon nanotubes (SWNT) coated electrode. The amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.1 versus Ag/AgCl to reduce the enzymatically produced H(2)O(2) with minimal interference from the coexisting electroactive compounds. The single-wall carbon nanotubes, sandwiched between the enzyme loading polypyrrole (PPy) layer and the conducting substrate (gold electrode), could efficiently promote the direct electron transfer of HRP. Operational characteristics of the bienzymatic sensor, in terms of linear range, detection limit, sensitivity, selectivity and stability, were presented in detail. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17764922/Bienzymatic_glucose_biosensor_based_on_co_immobilization_of_peroxidase_and_glucose_oxidase_on_a_carbon_nanotubes_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00280-1 DB - PRIME DP - Unbound Medicine ER -