Tags

Type your tag names separated by a space and hit enter

Urinary analysis of 8-oxoguanine, 8-oxoguanosine, fapy-guanine and 8-oxo-2'-deoxyguanosine by high-performance liquid chromatography-electrospray tandem mass spectrometry as a measure of oxidative stress.
J Chromatogr A. 2007 Oct 05; 1167(1):54-62.JC

Abstract

A sensitive and specific assay aimed at measuring the oxidized nucleic acids, 8-oxoguanine (8-oxoGua), fapy-guanine (Fapy-Gua), 8-oxoguanosine (8-oxoGuo), 8-oxo-2'-deoxyguanosine (8-oxodG) has been developed by coupling reversed phase liquid chromatography (HPLC) with electrospray tandem mass spectrometry detection (MS/MS) and isotope dilution. The HPLC-MS/MS approach with multiple reaction monitoring (MRM) allowed for the sensitive determination of 8-oxoGua, Fapy-Gua, 8-oxoGuo, and 8-oxodG in human urine samples. There is no sample preparation needed except for the addition of buffer and (13)C- and (15)N-labeled internal standards to the urine prior to sample injection into the HPLC-MS/MS system. This method was tested in urine samples from non-smokers, smokers, non-smokers with chronic kidney disease (CKD) and smokers with CKD, to assess the level of oxidative damage to nucleic acids. Markers of both RNA and DNA damage were significantly increased in the smokers with and without CKD compared to their respective control subjects. These findings suggest that a highly specific and sensitive analytical method such as isotope dilution HPLC-MS/MS may represent a valuable tool for the measurement of oxidative stress in human subjects.

Links

Publisher Full Text

Authors+Show Affiliations

Malayappan B
Department of Aging and Geriatrics, College of Medicine, Institute on Aging, Division of Biology of Aging, University of Florida, Gainesville, FL 32611, USA.
Garrett TJ
No affiliation info available
Segal M
No affiliation info available
Leeuwenburgh C
No affiliation info available

MeSH

8-Hydroxy-2'-DeoxyguanosineCarbon IsotopesChromatography, High Pressure LiquidDNA DamageDeoxyguanosineGuanineGuanosineHumansIndicator Dilution TechniquesKidney Failure, ChronicMolecular StructureNitrogen IsotopesOxidation-ReductionOxidative StressPyrimidinesReference StandardsSmokingSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17765254

Citation

Malayappan, Bhaskar, et al. "Urinary Analysis of 8-oxoguanine, 8-oxoguanosine, Fapy-guanine and 8-oxo-2'-deoxyguanosine By High-performance Liquid Chromatography-electrospray Tandem Mass Spectrometry as a Measure of Oxidative Stress." Journal of Chromatography. A, vol. 1167, no. 1, 2007, pp. 54-62.
Malayappan B, Garrett TJ, Segal M, et al. Urinary analysis of 8-oxoguanine, 8-oxoguanosine, fapy-guanine and 8-oxo-2'-deoxyguanosine by high-performance liquid chromatography-electrospray tandem mass spectrometry as a measure of oxidative stress. J Chromatogr A. 2007;1167(1):54-62.
Malayappan, B., Garrett, T. J., Segal, M., & Leeuwenburgh, C. (2007). Urinary analysis of 8-oxoguanine, 8-oxoguanosine, fapy-guanine and 8-oxo-2'-deoxyguanosine by high-performance liquid chromatography-electrospray tandem mass spectrometry as a measure of oxidative stress. Journal of Chromatography. A, 1167(1), 54-62.
Malayappan B, et al. Urinary Analysis of 8-oxoguanine, 8-oxoguanosine, Fapy-guanine and 8-oxo-2'-deoxyguanosine By High-performance Liquid Chromatography-electrospray Tandem Mass Spectrometry as a Measure of Oxidative Stress. J Chromatogr A. 2007 Oct 5;1167(1):54-62. PubMed PMID: 17765254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urinary analysis of 8-oxoguanine, 8-oxoguanosine, fapy-guanine and 8-oxo-2'-deoxyguanosine by high-performance liquid chromatography-electrospray tandem mass spectrometry as a measure of oxidative stress. AU - Malayappan,Bhaskar, AU - Garrett,Timothy J, AU - Segal,Mark, AU - Leeuwenburgh,Christiaan, Y1 - 2007/08/17/ PY - 2007/06/12/received PY - 2007/08/06/revised PY - 2007/08/13/accepted PY - 2007/9/4/pubmed PY - 2007/12/7/medline PY - 2007/9/4/entrez SP - 54 EP - 62 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1167 IS - 1 N2 - A sensitive and specific assay aimed at measuring the oxidized nucleic acids, 8-oxoguanine (8-oxoGua), fapy-guanine (Fapy-Gua), 8-oxoguanosine (8-oxoGuo), 8-oxo-2'-deoxyguanosine (8-oxodG) has been developed by coupling reversed phase liquid chromatography (HPLC) with electrospray tandem mass spectrometry detection (MS/MS) and isotope dilution. The HPLC-MS/MS approach with multiple reaction monitoring (MRM) allowed for the sensitive determination of 8-oxoGua, Fapy-Gua, 8-oxoGuo, and 8-oxodG in human urine samples. There is no sample preparation needed except for the addition of buffer and (13)C- and (15)N-labeled internal standards to the urine prior to sample injection into the HPLC-MS/MS system. This method was tested in urine samples from non-smokers, smokers, non-smokers with chronic kidney disease (CKD) and smokers with CKD, to assess the level of oxidative damage to nucleic acids. Markers of both RNA and DNA damage were significantly increased in the smokers with and without CKD compared to their respective control subjects. These findings suggest that a highly specific and sensitive analytical method such as isotope dilution HPLC-MS/MS may represent a valuable tool for the measurement of oxidative stress in human subjects. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17765254/Urinary_analysis_of_8_oxoguanine_8_oxoguanosine_fapy_guanine_and_8_oxo_2'_deoxyguanosine_by_high_performance_liquid_chromatography_electrospray_tandem_mass_spectrometry_as_a_measure_of_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)01385-4 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓13]

Related Citations

More