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Analyses of macrolide antibiotic residues in eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2007; 21(19):3213-22.RC

Abstract

Two liquid chromatography mass spectrometric techniques, i.e. ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-Tof MS) and high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS), were used for quantification, confirmation or identification of six macrolide antibiotic residues and/or their degradation products in eggs, raw milk, and/or honey. Macrolides were extracted from food samples by acetonitrile or phosphate buffer (0.1 M, pH 8.0), and sample extracts were further cleaned up using solid-phase extraction cartridges. UPLC/Q-Tof data were acquired in Tof MS full scan mode that allowed both quantification and confirmation of macrolides, and identification of their degradation products. LC/MS/MS data acquisition was achieved using multiple reaction monitoring (MRM), i.e. two transitions, to provide a high degree of sensitivity and repeatability. Matrix-matched standard calibration curves with the use of roxithromycin as an internal standard were utilized to achieve the best accuracy of the method. Both techniques demonstrated good quantitative performance in terms of accuracy and repeatability. LC/MS/MS had advantages over UPLC/Q-Tof MS in that its limits of detection were lower and repeatability was somewhat better. UPLC/Q-Tof provided ultimate and unequivocal confirmation of positive findings, and allowed degradation product identification based on accurate mass. The combination of the two techniques can be very beneficial or complementary in routine analysis of macrolide antibiotic residues and their degradation products in food matrices to ensure the safety of food supply.

Authors+Show Affiliations

Canadian Food Inspection Agency, Calgary Laboratory, 3650-36th Street N.W., Calgary, Alberta, Canada. wangj@inspection.gc.caNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17768705

Citation

Wang, Jian, and Daniel Leung. "Analyses of Macrolide Antibiotic Residues in Eggs, Raw Milk, and Honey Using Both Ultra-performance Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry and High-performance Liquid Chromatography/tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 19, 2007, pp. 3213-22.
Wang J, Leung D. Analyses of macrolide antibiotic residues in eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2007;21(19):3213-22.
Wang, J., & Leung, D. (2007). Analyses of macrolide antibiotic residues in eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 21(19), 3213-22.
Wang J, Leung D. Analyses of Macrolide Antibiotic Residues in Eggs, Raw Milk, and Honey Using Both Ultra-performance Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry and High-performance Liquid Chromatography/tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2007;21(19):3213-22. PubMed PMID: 17768705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analyses of macrolide antibiotic residues in eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry. AU - Wang,Jian, AU - Leung,Daniel, PY - 2007/9/5/pubmed PY - 2007/11/14/medline PY - 2007/9/5/entrez SP - 3213 EP - 22 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 19 N2 - Two liquid chromatography mass spectrometric techniques, i.e. ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-Tof MS) and high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS), were used for quantification, confirmation or identification of six macrolide antibiotic residues and/or their degradation products in eggs, raw milk, and/or honey. Macrolides were extracted from food samples by acetonitrile or phosphate buffer (0.1 M, pH 8.0), and sample extracts were further cleaned up using solid-phase extraction cartridges. UPLC/Q-Tof data were acquired in Tof MS full scan mode that allowed both quantification and confirmation of macrolides, and identification of their degradation products. LC/MS/MS data acquisition was achieved using multiple reaction monitoring (MRM), i.e. two transitions, to provide a high degree of sensitivity and repeatability. Matrix-matched standard calibration curves with the use of roxithromycin as an internal standard were utilized to achieve the best accuracy of the method. Both techniques demonstrated good quantitative performance in terms of accuracy and repeatability. LC/MS/MS had advantages over UPLC/Q-Tof MS in that its limits of detection were lower and repeatability was somewhat better. UPLC/Q-Tof provided ultimate and unequivocal confirmation of positive findings, and allowed degradation product identification based on accurate mass. The combination of the two techniques can be very beneficial or complementary in routine analysis of macrolide antibiotic residues and their degradation products in food matrices to ensure the safety of food supply. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17768705/Analyses_of_macrolide_antibiotic_residues_in_eggs_raw_milk_and_honey_using_both_ultra_performance_liquid_chromatography/quadrupole_time_of_flight_mass_spectrometry_and_high_performance_liquid_chromatography/tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3207 DB - PRIME DP - Unbound Medicine ER -