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Effect of serum treatment on pertussis antibody determination by ELISA.
Dev Biol Stand. 1991; 73:175-84.DB

Abstract

Determination of anti-PT and anti-FHA antibodies by ELISA and neutralization titer by CHO-cell method were performed with inactivated and uninactivated human sera. The findings were as follows: in inactivated sera, higher anti-PT ELISA antibody titers were shown compared with the titers determined with corresponding inactivated sera, especially in sera having low ELISA antibody titers; however, anti-FHA ELISA antibody titers remained identical, with the uninactivated or inactivated sera. CHO-cell neutralization titer remained identical and stable between the uninactivated and inactivated sea. CHO-cell neutralization titers correlated with anti-PT ELISA antibody titers in sera without inactivation. Anti-PT ELISA antibody titers in inactivated sera, however, did not correlate with the corresponding CHO-cell neutralization titers. These results suggest the possible existence of (a) substance(s) with affinity to PT which enhance(s) the ELISA reaction when sera are inactivated. Therefore, the inactivation of sera is not favorable for anti-PT determination by ELISA.

Authors+Show Affiliations

Research Foundation of Microbial Diseases, Osaka University.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1778311

Citation

Tada, Y, et al. "Effect of Serum Treatment On Pertussis Antibody Determination By ELISA." Developments in Biological Standardization, vol. 73, 1991, pp. 175-84.
Tada Y, Ishikawa T, Chazono M, et al. Effect of serum treatment on pertussis antibody determination by ELISA. Dev Biol Stand. 1991;73:175-84.
Tada, Y., Ishikawa, T., Chazono, M., Yoshida, I., & Nii, R. (1991). Effect of serum treatment on pertussis antibody determination by ELISA. Developments in Biological Standardization, 73, 175-84.
Tada Y, et al. Effect of Serum Treatment On Pertussis Antibody Determination By ELISA. Dev Biol Stand. 1991;73:175-84. PubMed PMID: 1778311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of serum treatment on pertussis antibody determination by ELISA. AU - Tada,Y, AU - Ishikawa,T, AU - Chazono,M, AU - Yoshida,I, AU - Nii,R, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 175 EP - 84 JF - Developments in biological standardization JO - Dev Biol Stand VL - 73 N2 - Determination of anti-PT and anti-FHA antibodies by ELISA and neutralization titer by CHO-cell method were performed with inactivated and uninactivated human sera. The findings were as follows: in inactivated sera, higher anti-PT ELISA antibody titers were shown compared with the titers determined with corresponding inactivated sera, especially in sera having low ELISA antibody titers; however, anti-FHA ELISA antibody titers remained identical, with the uninactivated or inactivated sera. CHO-cell neutralization titer remained identical and stable between the uninactivated and inactivated sea. CHO-cell neutralization titers correlated with anti-PT ELISA antibody titers in sera without inactivation. Anti-PT ELISA antibody titers in inactivated sera, however, did not correlate with the corresponding CHO-cell neutralization titers. These results suggest the possible existence of (a) substance(s) with affinity to PT which enhance(s) the ELISA reaction when sera are inactivated. Therefore, the inactivation of sera is not favorable for anti-PT determination by ELISA. SN - 0301-5149 UR - https://www.unboundmedicine.com/medline/citation/1778311/Effect_of_serum_treatment_on_pertussis_antibody_determination_by_ELISA_ DB - PRIME DP - Unbound Medicine ER -