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Effects of a 3-day fast on regional lipid and glucose metabolism in human skeletal muscle and adipose tissue.
Acta Physiol (Oxf). 2007 Nov; 191(3):205-16.AP

Abstract

AIM

Fasting is characterized by increased whole body lipolysis and lipid oxidation, decreased glucose oxidation and insulin resistance. To identify the regional sources and underlying mechanisms, we studied 10 healthy male volunteers post-absorptively and after 72 h of fasting.

METHODS

Each study comprised a 3-h basal period and a 3-h hyperinsulinaemic euglycaemic clamp and we used a combination of leg and forearm arteriovenous techniques, upper and lower body microdialysis and glucose and palmitate tracers.

RESULTS

In the basal state, plasma levels, fluxes and oxidation rates of free fatty acids all roughly doubled after fasting. Palmitate fluxes across the forearm and leg also increased by two to threefold and interstitial leg muscle glycerol concentrations doubled. Subcutaneous femoral glycerol concentrations and blood flows were unaltered, but abdominal subcutaneous blood flow increased by 50% in the presence of unchanged glycerol concentrations, indicating stimulated abdominal lipolysis. During the clamp, we observed whole body insulin resistance and glucose uptake across the leg and forearm decreased by 60%.

CONCLUSION

Our data show that fasting induces insulin resistance in upper and lower body muscles and suggest that increased lipolysis, is primarily due to the activation of lipolysis in muscle-associated fat (in the leg) and in upper body subcutaneous fat, whereas peripheral subcutaneous fat is spared.

Authors+Show Affiliations

Department of Anaesthesia and Intensive Care Medicine, Aarhus University Hospital, Noerrebrogade, Aarhus, Denmark. jakobgjedsted@dadlnet.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17784905

Citation

Gjedsted, J, et al. "Effects of a 3-day Fast On Regional Lipid and Glucose Metabolism in Human Skeletal Muscle and Adipose Tissue." Acta Physiologica (Oxford, England), vol. 191, no. 3, 2007, pp. 205-16.
Gjedsted J, Gormsen LC, Nielsen S, et al. Effects of a 3-day fast on regional lipid and glucose metabolism in human skeletal muscle and adipose tissue. Acta Physiol (Oxf). 2007;191(3):205-16.
Gjedsted, J., Gormsen, L. C., Nielsen, S., Schmitz, O., Djurhuus, C. B., Keiding, S., Ørskov, H., Tønnesen, E., & Møller, N. (2007). Effects of a 3-day fast on regional lipid and glucose metabolism in human skeletal muscle and adipose tissue. Acta Physiologica (Oxford, England), 191(3), 205-16.
Gjedsted J, et al. Effects of a 3-day Fast On Regional Lipid and Glucose Metabolism in Human Skeletal Muscle and Adipose Tissue. Acta Physiol (Oxf). 2007;191(3):205-16. PubMed PMID: 17784905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a 3-day fast on regional lipid and glucose metabolism in human skeletal muscle and adipose tissue. AU - Gjedsted,J, AU - Gormsen,L C, AU - Nielsen,S, AU - Schmitz,O, AU - Djurhuus,C B, AU - Keiding,S, AU - Ørskov,H, AU - Tønnesen,E, AU - Møller,N, Y1 - 2007/09/03/ PY - 2007/9/6/pubmed PY - 2008/4/1/medline PY - 2007/9/6/entrez SP - 205 EP - 16 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 191 IS - 3 N2 - AIM: Fasting is characterized by increased whole body lipolysis and lipid oxidation, decreased glucose oxidation and insulin resistance. To identify the regional sources and underlying mechanisms, we studied 10 healthy male volunteers post-absorptively and after 72 h of fasting. METHODS: Each study comprised a 3-h basal period and a 3-h hyperinsulinaemic euglycaemic clamp and we used a combination of leg and forearm arteriovenous techniques, upper and lower body microdialysis and glucose and palmitate tracers. RESULTS: In the basal state, plasma levels, fluxes and oxidation rates of free fatty acids all roughly doubled after fasting. Palmitate fluxes across the forearm and leg also increased by two to threefold and interstitial leg muscle glycerol concentrations doubled. Subcutaneous femoral glycerol concentrations and blood flows were unaltered, but abdominal subcutaneous blood flow increased by 50% in the presence of unchanged glycerol concentrations, indicating stimulated abdominal lipolysis. During the clamp, we observed whole body insulin resistance and glucose uptake across the leg and forearm decreased by 60%. CONCLUSION: Our data show that fasting induces insulin resistance in upper and lower body muscles and suggest that increased lipolysis, is primarily due to the activation of lipolysis in muscle-associated fat (in the leg) and in upper body subcutaneous fat, whereas peripheral subcutaneous fat is spared. SN - 1748-1708 UR - https://www.unboundmedicine.com/medline/citation/17784905/Effects_of_a_3_day_fast_on_regional_lipid_and_glucose_metabolism_in_human_skeletal_muscle_and_adipose_tissue_ L2 - https://doi.org/10.1111/j.1748-1716.2007.01740.x DB - PRIME DP - Unbound Medicine ER -