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Dietary lupin protein lowers triglyceride concentrations in liver and plasma in rats by reducing hepatic gene expression of sterol regulatory element-binding protein-1c.
Ann Nutr Metab 2007; 51(4):387-92AN

Abstract

BACKGROUND

Recently, it has been shown that dietary lupin protein lowers plasma triglyceride concentrations in rats. In this study, we investigated the hypothesis that this effect is due to a downregulation of sterol regulatory element-binding protein (SREBP)-1c, a transcription factor that regulates the expression of lipogenic enzymes in the livers of rats.

METHODS

Two groups of 12 rats each were fed semisynthetic diets containing 200 g/kg of either casein (control group) or lupin protein from Lupinus albus for 22 days.

RESULTS

Rats fed the diet containing lupin protein had lower concentrations of triglycerides in the liver, plasma and VLDL + chylomicrons (p < 0.05). The concentration of protein in VLDL + chylomicrons was also lower in rats fed lupin protein than in rats fed casein (p < 0.05). The mRNA concentrations of SREBP-1c and fatty acid synthase in the liver were lower in rats fed lupin protein than in rats fed casein (p < 0.05). The mRNA concentrations of lipoprotein lipase in the liver did not differ between both groups of rats.

CONCLUSION

This study confirms that a protein isolated from L. albus is strongly hypotriglyceridemic in rats. It is shown for the first time that this effect is at least in part due to a downregulation of SREBP-1c in the liver which in turn leads to a reduction in hepatic fatty acid synthesis.

Authors+Show Affiliations

Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17785965

Citation

Spielmann, Julia, et al. "Dietary Lupin Protein Lowers Triglyceride Concentrations in Liver and Plasma in Rats By Reducing Hepatic Gene Expression of Sterol Regulatory Element-binding Protein-1c." Annals of Nutrition & Metabolism, vol. 51, no. 4, 2007, pp. 387-92.
Spielmann J, Shukla A, Brandsch C, et al. Dietary lupin protein lowers triglyceride concentrations in liver and plasma in rats by reducing hepatic gene expression of sterol regulatory element-binding protein-1c. Ann Nutr Metab. 2007;51(4):387-92.
Spielmann, J., Shukla, A., Brandsch, C., Hirche, F., Stangl, G. I., & Eder, K. (2007). Dietary lupin protein lowers triglyceride concentrations in liver and plasma in rats by reducing hepatic gene expression of sterol regulatory element-binding protein-1c. Annals of Nutrition & Metabolism, 51(4), pp. 387-92.
Spielmann J, et al. Dietary Lupin Protein Lowers Triglyceride Concentrations in Liver and Plasma in Rats By Reducing Hepatic Gene Expression of Sterol Regulatory Element-binding Protein-1c. Ann Nutr Metab. 2007;51(4):387-92. PubMed PMID: 17785965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary lupin protein lowers triglyceride concentrations in liver and plasma in rats by reducing hepatic gene expression of sterol regulatory element-binding protein-1c. AU - Spielmann,Julia, AU - Shukla,Anjali, AU - Brandsch,Corinna, AU - Hirche,Frank, AU - Stangl,Gabriele I, AU - Eder,Klaus, Y1 - 2007/08/30/ PY - 2007/03/20/received PY - 2007/05/02/accepted PY - 2007/9/6/pubmed PY - 2007/10/13/medline PY - 2007/9/6/entrez SP - 387 EP - 92 JF - Annals of nutrition & metabolism JO - Ann. Nutr. Metab. VL - 51 IS - 4 N2 - BACKGROUND: Recently, it has been shown that dietary lupin protein lowers plasma triglyceride concentrations in rats. In this study, we investigated the hypothesis that this effect is due to a downregulation of sterol regulatory element-binding protein (SREBP)-1c, a transcription factor that regulates the expression of lipogenic enzymes in the livers of rats. METHODS: Two groups of 12 rats each were fed semisynthetic diets containing 200 g/kg of either casein (control group) or lupin protein from Lupinus albus for 22 days. RESULTS: Rats fed the diet containing lupin protein had lower concentrations of triglycerides in the liver, plasma and VLDL + chylomicrons (p < 0.05). The concentration of protein in VLDL + chylomicrons was also lower in rats fed lupin protein than in rats fed casein (p < 0.05). The mRNA concentrations of SREBP-1c and fatty acid synthase in the liver were lower in rats fed lupin protein than in rats fed casein (p < 0.05). The mRNA concentrations of lipoprotein lipase in the liver did not differ between both groups of rats. CONCLUSION: This study confirms that a protein isolated from L. albus is strongly hypotriglyceridemic in rats. It is shown for the first time that this effect is at least in part due to a downregulation of SREBP-1c in the liver which in turn leads to a reduction in hepatic fatty acid synthesis. SN - 1421-9697 UR - https://www.unboundmedicine.com/medline/citation/17785965/Dietary_lupin_protein_lowers_triglyceride_concentrations_in_liver_and_plasma_in_rats_by_reducing_hepatic_gene_expression_of_sterol_regulatory_element_binding_protein_1c_ L2 - https://www.karger.com?DOI=10.1159/000107720 DB - PRIME DP - Unbound Medicine ER -