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BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype.
Cytotherapy. 2007; 9(5):439-50.C

Abstract

BACKGROUND

Mesenchymal stromal cells (MSC) isolated from adult human BM are characterized by their fibroblast-like morphology, adherent growth and capacity to differentiate into adipocytes, osteocytes, chondrocytes, cardiomyocytes and neuroprogenitors. After culturing these cells in vitro, they express the cell-surface molecules CD44, CD90, SH2 and SH3, and are negative for CD34 and the hematopoietic marker CD45. The aim of this study was to characterize the in vivo phenotype of MSC relative to the expression of CD34 and CD45.

METHODS

BM mononuclear cells were stained with Ab against both molecules and separated into the CD34(+), CD34(-), CD45(+) CD34(+), CD45(high+) CD34(-), CD45(med,low+) CD34(-) and CD45(-) CD34(-) subpopulations, which were then cultured under the same conditions and analyzed for growth of MSC.

RESULTS

A small population of MSC arose from the CD45(+) CD34(+) fraction, although the majority was obtained from the CD45(-) CD34(-) subpopulation. MSC from all fractions could be differentiated into adipocytes and osteocytes. In addition, MSC from the CD34(+) and CD34(-) fractions were shown to differentiate into chondrocytes. After in vitro culture, MSC from both fractions possessed the same phenotype, which was negative for CD34 and CD45.

DISCUSSION

MSC from the CD45(+) CD34(+) fraction change their phenotype under in vitro conditions.

Authors+Show Affiliations

Department of Hematology/Oncology, University Medical Center, Freiburg, Germany. stefankaiser2@gmx.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17786605

Citation

Kaiser, S, et al. "BM Cells Giving Rise to MSC in Culture Have a Heterogeneous CD34 and CD45 Phenotype." Cytotherapy, vol. 9, no. 5, 2007, pp. 439-50.
Kaiser S, Hackanson B, Follo M, et al. BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype. Cytotherapy. 2007;9(5):439-50.
Kaiser, S., Hackanson, B., Follo, M., Mehlhorn, A., Geiger, K., Ihorst, G., & Kapp, U. (2007). BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype. Cytotherapy, 9(5), 439-50.
Kaiser S, et al. BM Cells Giving Rise to MSC in Culture Have a Heterogeneous CD34 and CD45 Phenotype. Cytotherapy. 2007;9(5):439-50. PubMed PMID: 17786605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype. AU - Kaiser,S, AU - Hackanson,B, AU - Follo,M, AU - Mehlhorn,A, AU - Geiger,K, AU - Ihorst,G, AU - Kapp,U, PY - 2007/9/6/pubmed PY - 2007/11/9/medline PY - 2007/9/6/entrez SP - 439 EP - 50 JF - Cytotherapy JO - Cytotherapy VL - 9 IS - 5 N2 - BACKGROUND: Mesenchymal stromal cells (MSC) isolated from adult human BM are characterized by their fibroblast-like morphology, adherent growth and capacity to differentiate into adipocytes, osteocytes, chondrocytes, cardiomyocytes and neuroprogenitors. After culturing these cells in vitro, they express the cell-surface molecules CD44, CD90, SH2 and SH3, and are negative for CD34 and the hematopoietic marker CD45. The aim of this study was to characterize the in vivo phenotype of MSC relative to the expression of CD34 and CD45. METHODS: BM mononuclear cells were stained with Ab against both molecules and separated into the CD34(+), CD34(-), CD45(+) CD34(+), CD45(high+) CD34(-), CD45(med,low+) CD34(-) and CD45(-) CD34(-) subpopulations, which were then cultured under the same conditions and analyzed for growth of MSC. RESULTS: A small population of MSC arose from the CD45(+) CD34(+) fraction, although the majority was obtained from the CD45(-) CD34(-) subpopulation. MSC from all fractions could be differentiated into adipocytes and osteocytes. In addition, MSC from the CD34(+) and CD34(-) fractions were shown to differentiate into chondrocytes. After in vitro culture, MSC from both fractions possessed the same phenotype, which was negative for CD34 and CD45. DISCUSSION: MSC from the CD45(+) CD34(+) fraction change their phenotype under in vitro conditions. SN - 1465-3249 UR - https://www.unboundmedicine.com/medline/citation/17786605/BM_cells_giving_rise_to_MSC_in_culture_have_a_heterogeneous_CD34_and_CD45_phenotype_ L2 - https://linkinghub.elsevier.com/retrieve/pii/778246531 DB - PRIME DP - Unbound Medicine ER -