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[Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis].
Zhongguo Zhong Yao Za Zhi. 2007 Jun; 32(12):1171-4.ZZ

Abstract

OBJECTIVE

To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.

METHOD

The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.

RESULT

The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and (-) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.

CONCLUSION

The method can be used to control the quality of Rhizoma fagopyri dibotoryis.

Authors+Show Affiliations

Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. ybinmm@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17802879

Citation

Yang, Bin, et al. "[Studies On HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 32, no. 12, 2007, pp. 1171-4.
Yang B, Xin MT, Lu DM, et al. [Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis]. Zhongguo Zhong Yao Za Zhi. 2007;32(12):1171-4.
Yang, B., Xin, M. T., Lu, D. M., Qu, Q. L., Li, H., & Huang, L. Q. (2007). [Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 32(12), 1171-4.
Yang B, et al. [Studies On HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis]. Zhongguo Zhong Yao Za Zhi. 2007;32(12):1171-4. PubMed PMID: 17802879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis]. AU - Yang,Bin, AU - Xin,Min-tong, AU - Lu,Dong-mei, AU - Qu,Qiao-ling, AU - Li,Hua, AU - Huang,Lu-qi, PY - 2007/9/7/pubmed PY - 2008/10/7/medline PY - 2007/9/7/entrez SP - 1171 EP - 4 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 32 IS - 12 N2 - OBJECTIVE: To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis. METHOD: The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun. RESULT: The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and (-) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively. CONCLUSION: The method can be used to control the quality of Rhizoma fagopyri dibotoryis. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/17802879/[Studies_on_HPLC_fingerprint_of_Rhizoma_Fagopyri_Dibotoryis]_ DB - PRIME DP - Unbound Medicine ER -