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[Determination of three glycosides from herbs of Swertia punicea by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2007 Jun; 32(12):1184-6.ZZ

Abstract

OBJECTIVE

To develop a RP-HPLC method for determination of three glycosides in Swertia punicea.

METHOD

Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C.

RESULT

The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).

CONCLUSION

It is a simple and sensitive meathod in controlling the quality of S. punicea.

Authors+Show Affiliations

Zhang XQ
Hubei College of Traditional Chinese Medicine, Wuhan 430061, China.
Huang FJ
No affiliation info available
Chen JC
No affiliation info available
Liu YW
No affiliation info available

MeSH

CalibrationChromatography, High Pressure LiquidDrugs, Chinese HerbalGlucosidesGlycosidesIridoid GlucosidesIridoidsPlants, MedicinalQuality ControlReproducibility of ResultsSwertiaXanthones

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17802883

Citation

Zhang, Xiu-qiao, et al. "[Determination of Three Glycosides From Herbs of Swertia Punicea By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 32, no. 12, 2007, pp. 1184-6.
Zhang XQ, Huang FJ, Chen JC, et al. [Determination of three glycosides from herbs of Swertia punicea by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2007;32(12):1184-6.
Zhang, X. Q., Huang, F. J., Chen, J. C., & Liu, Y. W. (2007). [Determination of three glycosides from herbs of Swertia punicea by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 32(12), 1184-6.
Zhang XQ, et al. [Determination of Three Glycosides From Herbs of Swertia Punicea By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2007;32(12):1184-6. PubMed PMID: 17802883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of three glycosides from herbs of Swertia punicea by RP-HPLC]. AU - Zhang,Xiu-qiao, AU - Huang,Feng-jiao, AU - Chen,Jiao-chun, AU - Liu,Yan-wen, PY - 2007/9/7/pubmed PY - 2008/10/7/medline PY - 2007/9/7/entrez SP - 1184 EP - 6 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 32 IS - 12 N2 - OBJECTIVE: To develop a RP-HPLC method for determination of three glycosides in Swertia punicea. METHOD: Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C. RESULT: The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5). CONCLUSION: It is a simple and sensitive meathod in controlling the quality of S. punicea. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/17802883/[Determination_of_three_glycosides_from_herbs_of_Swertia_punicea_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -