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Direct determination of metformin in urine by adsorptive catalytic square-wave voltammetry.
J Pharm Biomed Anal. 2007 Oct 18; 45(2):275-81.JP

Abstract

A new adsorptive catalytic voltammetric method for voltammetric determination of metformin based on the catalytic hydrogen evolution reaction at a hanging mercury drop electrode was developed. The electrode reaction was analyzed under conditions of linear sweep voltammetry (LSV), differential pulse voltammetry (DPV) and Osteryoung-type square-wave voltammetry (SWV). The peak current depends on pH of the medium, concentration and chemical composition of the buffer solution, and instrumental parameters. The optimal conditions for quantitative determination were obtained in an acetate buffer at pH 4.7. The voltammetric procedure was characterized with respect to the repeatability, precision and the recovery. The detection and quantification limits were found to be 1.8 x 10(-8) and 5.9 x 10(-8) mol l(-1) for SWV, 3.2 x 10(-8) and 1.0 x 10(-7) mol l(-1) for DPV, and 7.7 x 10(-8) and 2.5 x 10(-7) mol l(-1) for LSV, respectively. The SW voltammetric method, as the most sensitive one, was applied for determination of metformin in human urine. The voltammetric method has been validated by using HPLC with UV detection.

Authors+Show Affiliations

Department of Instrumental Analysis, University of Łódź, Pomorska 163, 90-236 Łódź, Poland. skrzpek@uni.lodz.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17822866

Citation

Skrzypek, Sławomira, et al. "Direct Determination of Metformin in Urine By Adsorptive Catalytic Square-wave Voltammetry." Journal of Pharmaceutical and Biomedical Analysis, vol. 45, no. 2, 2007, pp. 275-81.
Skrzypek S, Mirceski V, Ciesielski W, et al. Direct determination of metformin in urine by adsorptive catalytic square-wave voltammetry. J Pharm Biomed Anal. 2007;45(2):275-81.
Skrzypek, S., Mirceski, V., Ciesielski, W., Sokołowski, A., & Zakrzewski, R. (2007). Direct determination of metformin in urine by adsorptive catalytic square-wave voltammetry. Journal of Pharmaceutical and Biomedical Analysis, 45(2), 275-81.
Skrzypek S, et al. Direct Determination of Metformin in Urine By Adsorptive Catalytic Square-wave Voltammetry. J Pharm Biomed Anal. 2007 Oct 18;45(2):275-81. PubMed PMID: 17822866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct determination of metformin in urine by adsorptive catalytic square-wave voltammetry. AU - Skrzypek,Sławomira, AU - Mirceski,Valentin, AU - Ciesielski,Witold, AU - Sokołowski,Adam, AU - Zakrzewski,Robert, Y1 - 2007/07/12/ PY - 2006/12/15/received PY - 2007/06/12/revised PY - 2007/07/06/accepted PY - 2007/9/8/pubmed PY - 2007/12/14/medline PY - 2007/9/8/entrez SP - 275 EP - 81 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 45 IS - 2 N2 - A new adsorptive catalytic voltammetric method for voltammetric determination of metformin based on the catalytic hydrogen evolution reaction at a hanging mercury drop electrode was developed. The electrode reaction was analyzed under conditions of linear sweep voltammetry (LSV), differential pulse voltammetry (DPV) and Osteryoung-type square-wave voltammetry (SWV). The peak current depends on pH of the medium, concentration and chemical composition of the buffer solution, and instrumental parameters. The optimal conditions for quantitative determination were obtained in an acetate buffer at pH 4.7. The voltammetric procedure was characterized with respect to the repeatability, precision and the recovery. The detection and quantification limits were found to be 1.8 x 10(-8) and 5.9 x 10(-8) mol l(-1) for SWV, 3.2 x 10(-8) and 1.0 x 10(-7) mol l(-1) for DPV, and 7.7 x 10(-8) and 2.5 x 10(-7) mol l(-1) for LSV, respectively. The SW voltammetric method, as the most sensitive one, was applied for determination of metformin in human urine. The voltammetric method has been validated by using HPLC with UV detection. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/17822866/Direct_determination_of_metformin_in_urine_by_adsorptive_catalytic_square_wave_voltammetry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(07)00402-5 DB - PRIME DP - Unbound Medicine ER -