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Prevalence of IgE-binding to Art v 1, Art v 4 and Amb a 1 in mugwort-allergic patients.
Int Arch Allergy Immunol 2008; 145(2):94-101IA

Abstract

BACKGROUND

Mugwort (Artemisia vulgaris) represents an important source of weed pollen allergens. The objectives of the present study were (i) to analyze the IgE binding profiles in a group of mugwort-allergic patients, (ii) to identify individual marker allergens crucial for the diagnosis of mugwort allergy and (iii) to identify potential crossreactive allergens present in ragweed (Ambrosia artemisiifolia) pollen extract.

METHODS

Sera from 100 pediatric mugwort-allergic patients were analyzed for their IgE binding pattern to natural mugwort and ragweed pollen proteins, purified natural and recombinant Art v 1, recombinant Art v 4 and recombinant Amb a 1 using immunoblots and ELISA.

RESULTS

91% of the patients' sera tested displayed IgE binding to one or more mugwort pollen allergens in ELISA and 88% were positive in immunoblot. Purified natural Art v 1 was recognized by 79%, the recombinant protein by 39% of the patients tested and purified recombinant Art v 4 by 34% of the patients' sera. 67% of the sera displayed crossreactive IgE to one or more ragweed pollen allergens. Recombinant Amb a 1 was noted in only 14% of the mugwort-allergic sera.

CONCLUSIONS

Allergen-specific in vitro diagnosis was performed in 100 pediatric mugwort-allergic serum samples. Using two allergens (Art v 1 and Art v 4), 91% of the patients could be identified as mugwort pollen-sensitized patients by IgE in vitro tests. Crossreactivity to ragweed pollen allergens was demonstrated by in vitro experiments, suggesting a new important and potent allergen source expanding across Europe.

Authors+Show Affiliations

Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17823540

Citation

Oberhuber, C, et al. "Prevalence of IgE-binding to Art V 1, Art V 4 and Amb a 1 in Mugwort-allergic Patients." International Archives of Allergy and Immunology, vol. 145, no. 2, 2008, pp. 94-101.
Oberhuber C, Ma Y, Wopfner N, et al. Prevalence of IgE-binding to Art v 1, Art v 4 and Amb a 1 in mugwort-allergic patients. Int Arch Allergy Immunol. 2008;145(2):94-101.
Oberhuber, C., Ma, Y., Wopfner, N., Gadermaier, G., Dedic, A., Niggemann, B., ... Hoffmann-Sommergruber, K. (2008). Prevalence of IgE-binding to Art v 1, Art v 4 and Amb a 1 in mugwort-allergic patients. International Archives of Allergy and Immunology, 145(2), pp. 94-101.
Oberhuber C, et al. Prevalence of IgE-binding to Art V 1, Art V 4 and Amb a 1 in Mugwort-allergic Patients. Int Arch Allergy Immunol. 2008;145(2):94-101. PubMed PMID: 17823540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of IgE-binding to Art v 1, Art v 4 and Amb a 1 in mugwort-allergic patients. AU - Oberhuber,C, AU - Ma,Y, AU - Wopfner,N, AU - Gadermaier,G, AU - Dedic,A, AU - Niggemann,B, AU - Maderegger,B, AU - Gruber,P, AU - Ferreira,F, AU - Scheiner,O, AU - Hoffmann-Sommergruber,K, Y1 - 2007/09/07/ PY - 2006/12/18/received PY - 2007/05/30/accepted PY - 2007/9/8/pubmed PY - 2008/2/13/medline PY - 2007/9/8/entrez SP - 94 EP - 101 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 145 IS - 2 N2 - BACKGROUND: Mugwort (Artemisia vulgaris) represents an important source of weed pollen allergens. The objectives of the present study were (i) to analyze the IgE binding profiles in a group of mugwort-allergic patients, (ii) to identify individual marker allergens crucial for the diagnosis of mugwort allergy and (iii) to identify potential crossreactive allergens present in ragweed (Ambrosia artemisiifolia) pollen extract. METHODS: Sera from 100 pediatric mugwort-allergic patients were analyzed for their IgE binding pattern to natural mugwort and ragweed pollen proteins, purified natural and recombinant Art v 1, recombinant Art v 4 and recombinant Amb a 1 using immunoblots and ELISA. RESULTS: 91% of the patients' sera tested displayed IgE binding to one or more mugwort pollen allergens in ELISA and 88% were positive in immunoblot. Purified natural Art v 1 was recognized by 79%, the recombinant protein by 39% of the patients tested and purified recombinant Art v 4 by 34% of the patients' sera. 67% of the sera displayed crossreactive IgE to one or more ragweed pollen allergens. Recombinant Amb a 1 was noted in only 14% of the mugwort-allergic sera. CONCLUSIONS: Allergen-specific in vitro diagnosis was performed in 100 pediatric mugwort-allergic serum samples. Using two allergens (Art v 1 and Art v 4), 91% of the patients could be identified as mugwort pollen-sensitized patients by IgE in vitro tests. Crossreactivity to ragweed pollen allergens was demonstrated by in vitro experiments, suggesting a new important and potent allergen source expanding across Europe. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/17823540/Prevalence_of_IgE_binding_to_Art_v_1_Art_v_4_and_Amb_a_1_in_mugwort_allergic_patients_ L2 - https://www.karger.com?DOI=10.1159/000108134 DB - PRIME DP - Unbound Medicine ER -