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Secreted complement regulatory protein clusterin interacts with dengue virus nonstructural protein 1.
Biochem Biophys Res Commun. 2007 Nov 03; 362(4):1051-6.BB

Abstract

Vascular leakage and shock are the major causes of death in patients with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It has been suggested that patients with an elevated level of the free soluble form of dengue virus (DV) nonstructural protein 1 (sNS1) are at risk of developing DHF. To understand the role of sNS1 in blood, we searched for the host molecule with which NS1 interacts in human plasma by affinity purification using a GST-fused NS1. Complement inhibitory factor clusterin (Clu), which naturally inhibits the formation of terminal complement complex (TCC), was identified by mass spectrometry. A recombinant sNS1 produced from 293T cells and sNS1 from DV-infected Vero cells interacted with human Clu. Since an activated complement system reportedly causes vascular leakage, the interaction between sNS1 and Clu may contribute to the progression of DHF.

Authors+Show Affiliations

Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Tiwanon Rd, Muang, Nonthaburi 11000, Thailand. tkurosu@biken.osaka-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17825259

Citation

Kurosu, Takeshi, et al. "Secreted Complement Regulatory Protein Clusterin Interacts With Dengue Virus Nonstructural Protein 1." Biochemical and Biophysical Research Communications, vol. 362, no. 4, 2007, pp. 1051-6.
Kurosu T, Chaichana P, Yamate M, et al. Secreted complement regulatory protein clusterin interacts with dengue virus nonstructural protein 1. Biochem Biophys Res Commun. 2007;362(4):1051-6.
Kurosu, T., Chaichana, P., Yamate, M., Anantapreecha, S., & Ikuta, K. (2007). Secreted complement regulatory protein clusterin interacts with dengue virus nonstructural protein 1. Biochemical and Biophysical Research Communications, 362(4), 1051-6.
Kurosu T, et al. Secreted Complement Regulatory Protein Clusterin Interacts With Dengue Virus Nonstructural Protein 1. Biochem Biophys Res Commun. 2007 Nov 3;362(4):1051-6. PubMed PMID: 17825259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Secreted complement regulatory protein clusterin interacts with dengue virus nonstructural protein 1. AU - Kurosu,Takeshi, AU - Chaichana,Panjaporn, AU - Yamate,Masanobu, AU - Anantapreecha,Surapee, AU - Ikuta,Kazuyoshi, Y1 - 2007/08/30/ PY - 2007/08/02/received PY - 2007/08/19/accepted PY - 2007/9/11/pubmed PY - 2007/12/14/medline PY - 2007/9/11/entrez SP - 1051 EP - 6 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 362 IS - 4 N2 - Vascular leakage and shock are the major causes of death in patients with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It has been suggested that patients with an elevated level of the free soluble form of dengue virus (DV) nonstructural protein 1 (sNS1) are at risk of developing DHF. To understand the role of sNS1 in blood, we searched for the host molecule with which NS1 interacts in human plasma by affinity purification using a GST-fused NS1. Complement inhibitory factor clusterin (Clu), which naturally inhibits the formation of terminal complement complex (TCC), was identified by mass spectrometry. A recombinant sNS1 produced from 293T cells and sNS1 from DV-infected Vero cells interacted with human Clu. Since an activated complement system reportedly causes vascular leakage, the interaction between sNS1 and Clu may contribute to the progression of DHF. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/17825259/Secreted_complement_regulatory_protein_clusterin_interacts_with_dengue_virus_nonstructural_protein_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(07)01834-7 DB - PRIME DP - Unbound Medicine ER -