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Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine.
Eur J Pharmacol. 2007 Dec 08; 576(1-3):143-50.EJ

Abstract

TRPA1 is a member of the transient receptor potential (TRP) channel family expressed in sensory neurons. The present study focused on the effects of TRPA1 activation on contractile responses in isolated mouse intestine preparations. The jejunum, ileum, and proximal and distal colon were surgically isolated from male ddY mice. Intestinal motility was recorded as changes in isotonic tension. TRPA1, TRPM8, and TRPV1 expressions were examined by reverse transcription-polymerase chain reaction (RT-PCR). A TRPA1 agonist allyl isothiocyanate (AITC) dose-dependently induced contractions in the proximal and distal colon, whereas in the jejunum and ileum, even 100 muM AITC caused very little contraction. Likewise, a TRPA1 and TRPM8 agonist icilin, a TRPA1 agonist allicin, and a TRPV1 agonist capsaicin induced contractions in the colon. However, a TRPM8 agonist menthol induced long-lasting relaxation in the colon. Repeated exposure to AITC produced desensitization of its own contraction in the colon. Moreover, contractions induced by AITC generate cross-desensitization with icilin and capsaicin. Tetrodotoxin completely abolished AITC-induced contractions in the colon, whereas atropine significantly attenuated AITC-induced contractions in the distal colon, but not in the proximal colon. Menthol-induced relaxation in the colon was not inhibited by tetrodotoxin and atropine. RT-PCR analysis revealed the expression of TRPA1 and TRPV1, but not TRPM8, throughout the mouse intestine. These results suggest that TRPA1, but not TRPM8, are functionally expressed in the enteric nervous system throughout the mouse intestine on neurons that may also co-express TRPV1, yet the contractile responses to TRPA1 activation differ depending on their location along the intestine.

Authors+Show Affiliations

Department of Molecular Pharmacology and Pharmacotherapeutics, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17825279

Citation

Penuelas, Angelica, et al. "Contractile Effect of TRPA1 Receptor Agonists in the Isolated Mouse Intestine." European Journal of Pharmacology, vol. 576, no. 1-3, 2007, pp. 143-50.
Penuelas A, Tashima K, Tsuchiya S, et al. Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine. Eur J Pharmacol. 2007;576(1-3):143-50.
Penuelas, A., Tashima, K., Tsuchiya, S., Matsumoto, K., Nakamura, T., Horie, S., & Yano, S. (2007). Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine. European Journal of Pharmacology, 576(1-3), 143-50.
Penuelas A, et al. Contractile Effect of TRPA1 Receptor Agonists in the Isolated Mouse Intestine. Eur J Pharmacol. 2007 Dec 8;576(1-3):143-50. PubMed PMID: 17825279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine. AU - Penuelas,Angelica, AU - Tashima,Kimihito, AU - Tsuchiya,Shizuko, AU - Matsumoto,Kenjiro, AU - Nakamura,Tomonori, AU - Horie,Syunji, AU - Yano,Shingo, Y1 - 2007/08/17/ PY - 2007/03/09/received PY - 2007/08/04/revised PY - 2007/08/13/accepted PY - 2007/9/11/pubmed PY - 2008/3/7/medline PY - 2007/9/11/entrez SP - 143 EP - 50 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 576 IS - 1-3 N2 - TRPA1 is a member of the transient receptor potential (TRP) channel family expressed in sensory neurons. The present study focused on the effects of TRPA1 activation on contractile responses in isolated mouse intestine preparations. The jejunum, ileum, and proximal and distal colon were surgically isolated from male ddY mice. Intestinal motility was recorded as changes in isotonic tension. TRPA1, TRPM8, and TRPV1 expressions were examined by reverse transcription-polymerase chain reaction (RT-PCR). A TRPA1 agonist allyl isothiocyanate (AITC) dose-dependently induced contractions in the proximal and distal colon, whereas in the jejunum and ileum, even 100 muM AITC caused very little contraction. Likewise, a TRPA1 and TRPM8 agonist icilin, a TRPA1 agonist allicin, and a TRPV1 agonist capsaicin induced contractions in the colon. However, a TRPM8 agonist menthol induced long-lasting relaxation in the colon. Repeated exposure to AITC produced desensitization of its own contraction in the colon. Moreover, contractions induced by AITC generate cross-desensitization with icilin and capsaicin. Tetrodotoxin completely abolished AITC-induced contractions in the colon, whereas atropine significantly attenuated AITC-induced contractions in the distal colon, but not in the proximal colon. Menthol-induced relaxation in the colon was not inhibited by tetrodotoxin and atropine. RT-PCR analysis revealed the expression of TRPA1 and TRPV1, but not TRPM8, throughout the mouse intestine. These results suggest that TRPA1, but not TRPM8, are functionally expressed in the enteric nervous system throughout the mouse intestine on neurons that may also co-express TRPV1, yet the contractile responses to TRPA1 activation differ depending on their location along the intestine. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/17825279/Contractile_effect_of_TRPA1_receptor_agonists_in_the_isolated_mouse_intestine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)00908-9 DB - PRIME DP - Unbound Medicine ER -