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Oxytocin ameliorates skin damage and oxidant gastric injury in rats with thermal trauma.
Burns. 2008 May; 34(3):361-9.B

Abstract

Transient splanchnic vasoconstriction following major burns causes oxidative and/or nitrosative damage in gastrointestinal tissues due to ischemia, which is followed by reperfusion injury. Oxytocin (OT), a hypothalamic nonapeptide, possesses antisecretory and antiulcer effects, facilitates wound healing and is involved in immune and inflammatory processes. To assess the possible protective effect of oxytocin (OT) against burn-induced gastric injury, Sprague-Dawley rats (250-300g) were randomly divided into three groups as control (n=8), OT-treated burn (n=8) and saline-treated burn (n=8) groups. Under anesthesia, the shaved dorsal skin of rats was exposed to 90 degrees C water for 10s to induce burn injury covering 30% of total body surface area in a standardized manner. Either oxytocin (5microg/kg) or saline was administered subcutaneously immediately after and at 24h following burn, and the rats were decapitated at 48h. Serum samples were assayed for TNF-alpha, and stomach was taken for the determination of malondialdehyde (MDA), myeloperoxidase (MPO) activity, DNA fragmentation rate (%) and histopathological examination. MDA and MPO were assayed for products of lipid peroxidation and as an index of tissue neutrophil infiltration, respectively. When compared to control group, burn caused significant increases in gastric MDA and MPO activity and increased microscopic damage scores at 48h (p<0.001). Oxytocin treatment reversed the burn-induced elevations in MDA and MPO levels and reduced the gastric damage scores (p<0.001, p<0.01), while TNF-alpha levels, which were increased significantly at 48thh after injury (p<0.001), were abolished with OT treatment (p<0.001). The results of this study suggest that oxytocin may provide a therapeutic benefit in diminishing burn-induced gastric inflammation by depressing tissue neutrophil infiltration and decreasing the release of inflammatory cytokines, but requires further investigation as a potential therapeutic agent in ameliorating the systemic effects of severe burn.

Authors+Show Affiliations

Marmara University, School of Medicine, Department of Physiology, Haydarpaşa, Istanbul 34668, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17826914

Citation

Işeri, Sevgin Ozlem, et al. "Oxytocin Ameliorates Skin Damage and Oxidant Gastric Injury in Rats With Thermal Trauma." Burns : Journal of the International Society for Burn Injuries, vol. 34, no. 3, 2008, pp. 361-9.
Işeri SO, Gedik IE, Erzik C, et al. Oxytocin ameliorates skin damage and oxidant gastric injury in rats with thermal trauma. Burns. 2008;34(3):361-9.
Işeri, S. O., Gedik, I. E., Erzik, C., Uslu, B., Arbak, S., Gedik, N., & Yeğen, B. C. (2008). Oxytocin ameliorates skin damage and oxidant gastric injury in rats with thermal trauma. Burns : Journal of the International Society for Burn Injuries, 34(3), 361-9.
Işeri SO, et al. Oxytocin Ameliorates Skin Damage and Oxidant Gastric Injury in Rats With Thermal Trauma. Burns. 2008;34(3):361-9. PubMed PMID: 17826914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxytocin ameliorates skin damage and oxidant gastric injury in rats with thermal trauma. AU - Işeri,Sevgin Ozlem, AU - Gedik,Ismail Ertuğrul, AU - Erzik,Can, AU - Uslu,Bahar, AU - Arbak,Serap, AU - Gedik,Nursal, AU - Yeğen,Berrak C, Y1 - 2007/09/10/ PY - 2006/10/04/received PY - 2007/03/29/accepted PY - 2007/9/11/pubmed PY - 2008/8/30/medline PY - 2007/9/11/entrez SP - 361 EP - 9 JF - Burns : journal of the International Society for Burn Injuries JO - Burns VL - 34 IS - 3 N2 - Transient splanchnic vasoconstriction following major burns causes oxidative and/or nitrosative damage in gastrointestinal tissues due to ischemia, which is followed by reperfusion injury. Oxytocin (OT), a hypothalamic nonapeptide, possesses antisecretory and antiulcer effects, facilitates wound healing and is involved in immune and inflammatory processes. To assess the possible protective effect of oxytocin (OT) against burn-induced gastric injury, Sprague-Dawley rats (250-300g) were randomly divided into three groups as control (n=8), OT-treated burn (n=8) and saline-treated burn (n=8) groups. Under anesthesia, the shaved dorsal skin of rats was exposed to 90 degrees C water for 10s to induce burn injury covering 30% of total body surface area in a standardized manner. Either oxytocin (5microg/kg) or saline was administered subcutaneously immediately after and at 24h following burn, and the rats were decapitated at 48h. Serum samples were assayed for TNF-alpha, and stomach was taken for the determination of malondialdehyde (MDA), myeloperoxidase (MPO) activity, DNA fragmentation rate (%) and histopathological examination. MDA and MPO were assayed for products of lipid peroxidation and as an index of tissue neutrophil infiltration, respectively. When compared to control group, burn caused significant increases in gastric MDA and MPO activity and increased microscopic damage scores at 48h (p<0.001). Oxytocin treatment reversed the burn-induced elevations in MDA and MPO levels and reduced the gastric damage scores (p<0.001, p<0.01), while TNF-alpha levels, which were increased significantly at 48thh after injury (p<0.001), were abolished with OT treatment (p<0.001). The results of this study suggest that oxytocin may provide a therapeutic benefit in diminishing burn-induced gastric inflammation by depressing tissue neutrophil infiltration and decreasing the release of inflammatory cytokines, but requires further investigation as a potential therapeutic agent in ameliorating the systemic effects of severe burn. SN - 0305-4179 UR - https://www.unboundmedicine.com/medline/citation/17826914/Oxytocin_ameliorates_skin_damage_and_oxidant_gastric_injury_in_rats_with_thermal_trauma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0305-4179(07)00103-9 DB - PRIME DP - Unbound Medicine ER -