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Sensitive high-performance liquid chromatography-tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15; 858(1-2):159-67.JC

Abstract

A method to determine total and free mycophenolic acid (MPA) and its metabolites, the phenolic (MPAG) and acyl (AcMPAG) glucuronides, using HPLC and mass spectrometry was developed. Mean recoveries in plasma and urine samples were >85%, and the lower limits of quantification for MPA, MPAG and AcMPAG were 0.05, 0.05 and 0.01 mg/L, respectively. For plasma, the assay was linear over 0.05-50 mg/L for MPA and MPAG, and from 0.01 to 10mg/L for AcMPAG. A validation study demonstrated good inter- and intra-day precision (CV<or=11%) and accuracy (bias<or=16%) and satisfactory specificity and stability. Pharmacokinetic parameters were assessed in plasma and urine from healthy volunteers after an oral dose of mycophenolate mofetil.

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Authors+Show Affiliations

Benoit-Biancamano MO
Canada Research Chair in Pharmacogenomics, Laboratory of Pharmacogenomics, Oncology and Molecular Endocrinology Research Center, CHUL Research Center and Faculty of Pharmacy, Laval University, G1V 4G2 Québec, Canada.
Caron P
No affiliation info available
Lévesque E
No affiliation info available
Delage R
No affiliation info available
Couture F
No affiliation info available
Guillemette C
No affiliation info available

MeSH

Chromatography, High Pressure LiquidGlucuronidesHumansMycophenolic AcidReproducibility of ResultsSolid Phase ExtractionTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17827076

Citation

Benoit-Biancamano, Marie-Odile, et al. "Sensitive High-performance Liquid Chromatography-tandem Mass Spectrometry Method for Quantitative Analysis of Mycophenolic Acid and Its Glucuronide Metabolites in Human Plasma and Urine." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 858, no. 1-2, 2007, pp. 159-67.
Benoit-Biancamano MO, Caron P, Lévesque E, et al. Sensitive high-performance liquid chromatography-tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;858(1-2):159-67.
Benoit-Biancamano, M. O., Caron, P., Lévesque, E., Delage, R., Couture, F., & Guillemette, C. (2007). Sensitive high-performance liquid chromatography-tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 858(1-2), 159-67.
Benoit-Biancamano MO, et al. Sensitive High-performance Liquid Chromatography-tandem Mass Spectrometry Method for Quantitative Analysis of Mycophenolic Acid and Its Glucuronide Metabolites in Human Plasma and Urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15;858(1-2):159-67. PubMed PMID: 17827076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive high-performance liquid chromatography-tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine. AU - Benoit-Biancamano,Marie-Odile, AU - Caron,Patrick, AU - Lévesque,Eric, AU - Delage,Robert, AU - Couture,Félix, AU - Guillemette,Chantal, Y1 - 2007/08/24/ PY - 2007/02/23/received PY - 2007/08/14/revised PY - 2007/08/16/accepted PY - 2007/9/11/pubmed PY - 2007/12/20/medline PY - 2007/9/11/entrez SP - 159 EP - 67 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 858 IS - 1-2 N2 - A method to determine total and free mycophenolic acid (MPA) and its metabolites, the phenolic (MPAG) and acyl (AcMPAG) glucuronides, using HPLC and mass spectrometry was developed. Mean recoveries in plasma and urine samples were >85%, and the lower limits of quantification for MPA, MPAG and AcMPAG were 0.05, 0.05 and 0.01 mg/L, respectively. For plasma, the assay was linear over 0.05-50 mg/L for MPA and MPAG, and from 0.01 to 10mg/L for AcMPAG. A validation study demonstrated good inter- and intra-day precision (CV<or=11%) and accuracy (bias<or=16%) and satisfactory specificity and stability. Pharmacokinetic parameters were assessed in plasma and urine from healthy volunteers after an oral dose of mycophenolate mofetil. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17827076/Sensitive_high_performance_liquid_chromatography_tandem_mass_spectrometry_method_for_quantitative_analysis_of_mycophenolic_acid_and_its_glucuronide_metabolites_in_human_plasma_and_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00595-8 DB - PRIME DP - Unbound Medicine ER -