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Determination of vigabatrin in human plasma by means of CE with LIF detection.
Electrophoresis. 2007 Oct; 28(19):3535-41.E

Abstract

A method has been developed for the quantitation of the antiepileptic drug vigabatrin (VGB) in human plasma. It is based on CE with LIF detection. The effect of the pH of the buffer and of N-methylglucamine (GLC) as BGE constituent was investigated. The final BGE consisted of 50 mM borate buffer, pH 9.0, with 100 mM GLC and enabled separation within 12 min at 20 kV voltage. An SPE procedure was used for the pretreatment of biological samples, based on mixed-mode lipophilic-cation exchange cartridges, followed by a derivatization step with 6-carboxyfluorescein-N-succinimidyl ester (CFSE). Fluorescence was excited by an Ar-ion laser (lambda(exc) = 488 nm). Linearity was observed in the 10-120 microg/mL plasma concentration range. Extraction yield was >96%, precision (expressed as RSD) <6.7% and accuracy (recovery) was between 97.0 and 101.6%. The method has been successfully applied to the analysis of VGB in plasma of epileptic patients undergoing therapy with the drug.

Authors+Show Affiliations

Pharmaco-Toxicological Analysis Laboratory, Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17828800

Citation

Musenga, Alessandro, et al. "Determination of Vigabatrin in Human Plasma By Means of CE With LIF Detection." Electrophoresis, vol. 28, no. 19, 2007, pp. 3535-41.
Musenga A, Mandrioli R, Comin I, et al. Determination of vigabatrin in human plasma by means of CE with LIF detection. Electrophoresis. 2007;28(19):3535-41.
Musenga, A., Mandrioli, R., Comin, I., Kenndler, E., & Raggi, M. A. (2007). Determination of vigabatrin in human plasma by means of CE with LIF detection. Electrophoresis, 28(19), 3535-41.
Musenga A, et al. Determination of Vigabatrin in Human Plasma By Means of CE With LIF Detection. Electrophoresis. 2007;28(19):3535-41. PubMed PMID: 17828800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of vigabatrin in human plasma by means of CE with LIF detection. AU - Musenga,Alessandro, AU - Mandrioli,Roberto, AU - Comin,Irene, AU - Kenndler,Ernst, AU - Raggi,Maria Augusta, PY - 2007/9/11/pubmed PY - 2008/2/7/medline PY - 2007/9/11/entrez SP - 3535 EP - 41 JF - Electrophoresis JO - Electrophoresis VL - 28 IS - 19 N2 - A method has been developed for the quantitation of the antiepileptic drug vigabatrin (VGB) in human plasma. It is based on CE with LIF detection. The effect of the pH of the buffer and of N-methylglucamine (GLC) as BGE constituent was investigated. The final BGE consisted of 50 mM borate buffer, pH 9.0, with 100 mM GLC and enabled separation within 12 min at 20 kV voltage. An SPE procedure was used for the pretreatment of biological samples, based on mixed-mode lipophilic-cation exchange cartridges, followed by a derivatization step with 6-carboxyfluorescein-N-succinimidyl ester (CFSE). Fluorescence was excited by an Ar-ion laser (lambda(exc) = 488 nm). Linearity was observed in the 10-120 microg/mL plasma concentration range. Extraction yield was >96%, precision (expressed as RSD) <6.7% and accuracy (recovery) was between 97.0 and 101.6%. The method has been successfully applied to the analysis of VGB in plasma of epileptic patients undergoing therapy with the drug. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17828800/Determination_of_vigabatrin_in_human_plasma_by_means_of_CE_with_LIF_detection_ L2 - https://doi.org/10.1002/elps.200700139 DB - PRIME DP - Unbound Medicine ER -