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Comparison of polymerase chain reaction technique with other methods for detection of Mycobacterium leprae in tissues of wild nine-banded armadillos.
Lepr Rev. 1991 Dec; 62(4):362-73.LR

Abstract

Thirty, nine-banded armadillos weighing between 3 and 5 kilograms trapped from an area endemic for armadillo leprosy were collected at random; killed, autopsied and examined histopathologically. Also, one of the right inguinal lymph nodes was removed under sterile precautions and examined using PCR, direct smear examination, mouse footpad study, culture in laboratory media and histopathology with a view to detecting Mycobacterium leprae. Blood was collected at death and tested for IgM antibodies to PGL-1. According to the PCR study of the inguinal lymph nodes 16 of 30 armadillos (53.3%) had evidence of M. leprae. Significant levels of IgM antibodies to PGL-1 and identifiable lepromatous granuloma in inguinal lymph nodes were found in 2 animals (6.7%) with advanced disseminated disease. The prevalence of generalized leprosy according to autopsy study was 13.3% and according to histopathological examination of ear tissue 3.3%. The presence of M. leprae in the tissues evoked no special tissue reaction in the early stages. The pattern of spread of the disease in 2 animals closely resembled that found in experimental animals infected intracutaneously. Initiation of infection by inoculation of M. leprae through thorn pricks remains a distinct possibility.

Authors+Show Affiliations

Laboratory Research Branch, Gillis W. Long Hansen's Disease Center, Carville, Louisiana 70721.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1784151

Citation

Job, C K., et al. "Comparison of Polymerase Chain Reaction Technique With Other Methods for Detection of Mycobacterium Leprae in Tissues of Wild Nine-banded Armadillos." Leprosy Review, vol. 62, no. 4, 1991, pp. 362-73.
Job CK, Drain V, Williams DL, et al. Comparison of polymerase chain reaction technique with other methods for detection of Mycobacterium leprae in tissues of wild nine-banded armadillos. Lepr Rev. 1991;62(4):362-73.
Job, C. K., Drain, V., Williams, D. L., Gillis, T. P., Truman, R. W., Sanchez, R. M., Deming, A. T., & Hastings, R. C. (1991). Comparison of polymerase chain reaction technique with other methods for detection of Mycobacterium leprae in tissues of wild nine-banded armadillos. Leprosy Review, 62(4), 362-73.
Job CK, et al. Comparison of Polymerase Chain Reaction Technique With Other Methods for Detection of Mycobacterium Leprae in Tissues of Wild Nine-banded Armadillos. Lepr Rev. 1991;62(4):362-73. PubMed PMID: 1784151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of polymerase chain reaction technique with other methods for detection of Mycobacterium leprae in tissues of wild nine-banded armadillos. AU - Job,C K, AU - Drain,V, AU - Williams,D L, AU - Gillis,T P, AU - Truman,R W, AU - Sanchez,R M, AU - Deming,A T, AU - Hastings,R C, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 362 EP - 73 JF - Leprosy review JO - Lepr Rev VL - 62 IS - 4 N2 - Thirty, nine-banded armadillos weighing between 3 and 5 kilograms trapped from an area endemic for armadillo leprosy were collected at random; killed, autopsied and examined histopathologically. Also, one of the right inguinal lymph nodes was removed under sterile precautions and examined using PCR, direct smear examination, mouse footpad study, culture in laboratory media and histopathology with a view to detecting Mycobacterium leprae. Blood was collected at death and tested for IgM antibodies to PGL-1. According to the PCR study of the inguinal lymph nodes 16 of 30 armadillos (53.3%) had evidence of M. leprae. Significant levels of IgM antibodies to PGL-1 and identifiable lepromatous granuloma in inguinal lymph nodes were found in 2 animals (6.7%) with advanced disseminated disease. The prevalence of generalized leprosy according to autopsy study was 13.3% and according to histopathological examination of ear tissue 3.3%. The presence of M. leprae in the tissues evoked no special tissue reaction in the early stages. The pattern of spread of the disease in 2 animals closely resembled that found in experimental animals infected intracutaneously. Initiation of infection by inoculation of M. leprae through thorn pricks remains a distinct possibility. SN - 0305-7518 UR - https://www.unboundmedicine.com/medline/citation/1784151/Comparison_of_polymerase_chain_reaction_technique_with_other_methods_for_detection_of_Mycobacterium_leprae_in_tissues_of_wild_nine_banded_armadillos_ DB - PRIME DP - Unbound Medicine ER -