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Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli.
Res Microbiol. 2007 Sep; 158(7):591-9.RM

Abstract

A collection of clinical Shiga-toxin-producing Escherichia coli (STEC) strains, mainly belonging to serotypes O26, O103, O111, O145 and O157, was characterised by a polyphasic approach including molecular serotyping, PCR-based detection of virulence factors (stx1, stx2, eae, EHEC-hlyA, saa, katP, espP), carbohydrate fermentation profiles using API50 tests and random amplification of polymorphic DNA (RAPD) fingerprinting. An RAPD protocol based on the combination of 2 primers resulted in sufficiently complex patterns enabling discrimination to the serotype level. Moreover, carbohydrate fermentation profiles obtained after evaluating up to 50 different carbohydrates led to separation of different STEC serotypes. Virulence typing results confirm the association of Shiga toxins and intimin subtypes with specific serotypes and clinical diagnosis. Clinical diagnosis of strains did not correlate with either RAPD profiles or carbohydrate fermentation patterns.

Authors+Show Affiliations

bjorn.posse@ugent.beUniversity of Ghent, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Hygiene and Technology, Salisburylaan 133, 9820 Merelbeke, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17845842

Citation

Possé, Björn, et al. "Metabolic and Genetic Profiling of Clinical O157 and non-O157 Shiga-toxin-producing Escherichia Coli." Research in Microbiology, vol. 158, no. 7, 2007, pp. 591-9.
Possé B, De Zutter L, Heyndrickx M, et al. Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli. Res Microbiol. 2007;158(7):591-9.
Possé, B., De Zutter, L., Heyndrickx, M., & Herman, L. (2007). Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli. Research in Microbiology, 158(7), 591-9.
Possé B, et al. Metabolic and Genetic Profiling of Clinical O157 and non-O157 Shiga-toxin-producing Escherichia Coli. Res Microbiol. 2007;158(7):591-9. PubMed PMID: 17845842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli. AU - Possé,Björn, AU - De Zutter,Lieven, AU - Heyndrickx,Marc, AU - Herman,Lieve, Y1 - 2007/06/19/ PY - 2007/04/30/received PY - 2007/06/06/revised PY - 2007/06/07/accepted PY - 2007/9/12/pubmed PY - 2008/1/8/medline PY - 2007/9/12/entrez SP - 591 EP - 9 JF - Research in microbiology JO - Res Microbiol VL - 158 IS - 7 N2 - A collection of clinical Shiga-toxin-producing Escherichia coli (STEC) strains, mainly belonging to serotypes O26, O103, O111, O145 and O157, was characterised by a polyphasic approach including molecular serotyping, PCR-based detection of virulence factors (stx1, stx2, eae, EHEC-hlyA, saa, katP, espP), carbohydrate fermentation profiles using API50 tests and random amplification of polymorphic DNA (RAPD) fingerprinting. An RAPD protocol based on the combination of 2 primers resulted in sufficiently complex patterns enabling discrimination to the serotype level. Moreover, carbohydrate fermentation profiles obtained after evaluating up to 50 different carbohydrates led to separation of different STEC serotypes. Virulence typing results confirm the association of Shiga toxins and intimin subtypes with specific serotypes and clinical diagnosis. Clinical diagnosis of strains did not correlate with either RAPD profiles or carbohydrate fermentation patterns. SN - 0923-2508 UR - https://www.unboundmedicine.com/medline/citation/17845842/Metabolic_and_genetic_profiling_of_clinical_O157_and_non_O157_Shiga_toxin_producing_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(07)00126-X DB - PRIME DP - Unbound Medicine ER -