TY - JOUR T1 - A high-efficiency capillary electrophoresis-based method for characterizing the sizes of Au nanoparticles. A1 - Liu,Fu-Ken, Y1 - 2007/08/31/ PY - 2007/08/03/received PY - 2007/08/19/revised PY - 2007/08/24/accepted PY - 2007/9/14/pubmed PY - 2007/11/8/medline PY - 2007/9/14/entrez SP - 231 EP - 5 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1167 IS - 2 N2 - In this paper, we present a highly efficient capillary electrophoresis (CE)-based method for characterizing the sizes of Au nanoparticles (NPs). We used the reversed electrode polarity stacking mode (REPSM) of CE for on-line enhancement of the detection and separation of Au NPs. Under the on-line enhancement separation conditions [buffer: SDS (70 mM) and 3-cyclohexylamino-1-propanesulfonic acid (10 mM) at pH 10.0; applied voltage: 20 kV; using the REPSM for sample concentration], the detection sensitivity toward the Au NPs improved by up to 260-fold. A linear relationship (R(2)=0.994) existed between the electrophoretic mobilities and the sizes of the Au NPs within the range of diameters from 5.3 to 59.9 nm; the relative standard deviations of the electrophoretic mobilities for these NPs were below 0.6%. When using these conditions to analyze Au NPs produced through seed-assisted synthesis, a good correlation existed between the sizes obtained using CE and those provided by transmission electron microscopy. Furthermore, the analyses performed using CE were rapid (<4 min) and required very low sample volumes (several nanoliters), yet they proceeded with high sensitivity. These findings confirm that CE is an efficient tool for characterizing the sizes of NPs. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17850805/A_high_efficiency_capillary_electrophoresis_based_method_for_characterizing_the_sizes_of_Au_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)01460-4 DB - PRIME DP - Unbound Medicine ER -