TY - JOUR T1 - Characterization and phylogeny of two beta-cytoskeletal actins from Hemibarbus mylodon (Cyprinidae, Cypriniformes), a threatened fish species in Korea. AU - Kim,Keun-Yong, AU - Lee,Sang Yoon, AU - Cho,Young Sun, AU - Bang,In Chul, AU - Kim,Dong Soo, AU - Nam,Yoon Kwon, PY - 2007/9/14/pubmed PY - 2008/9/19/medline PY - 2007/9/14/entrez SP - 87 EP - 97 JF - DNA sequence : the journal of DNA sequencing and mapping JO - DNA Seq VL - 19 IS - 2 N2 - Complementary DNA and genomic sequences representing two different beta-actins were isolated from a threatened freshwater fish species Hemibarbus mylodon. The beta-actin 1 and 2 encoded an identical number of amino acids (375 aa), and shared 88.8 and 99.7% of identity at coding nucleotide and amino acid levels, respectively. Genomic open reading frame (ORF) sequences of both isoforms contained five translated exons interrupted by four introns with conserved GT/AG exon/intron boundary rule. Semi-quantitative RT-PCR showed that the two isoform mRNAs were ubiquitously detected in all tissues tested, but transcript levels were variable across tissues. Phylogenetic analysis showed that H. mylodon beta-actin 1 and 2 were clustered into two distinct major and minor branches of Cypriniformes, respectively. Comparisons of the 5'-upstream region and 3'-UTR of H. mylodon beta-actin 1 also showed a high degree of homology with those of the major teleost beta-actins and warmblooded vertebrate beta-cytoskeletal actins, suggesting their more recent common origin. SN - 1042-5179 UR - https://www.unboundmedicine.com/medline/citation/17852350/Characterization_and_phylogeny_of_two_beta_cytoskeletal_actins_from_Hemibarbus_mylodon__Cyprinidae_Cypriniformes__a_threatened_fish_species_in_Korea_ DB - PRIME DP - Unbound Medicine ER -