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Interface membrane fibroblasts around aseptically loosened endoprostheses express MMP-13.
J Orthop Res. 2008 Feb; 26(2):143-52.JO

Abstract

The objective of this article was to assess whether matrix metalloproteinase-13 (MMP-13) is produced by cells of the peri-implant interface tissues and to further characterize these cells. Tissue specimens were collected from the bone-prosthesis interface at the time of revision surgery of clinically loosened hip and knee arthroplasties (n = 27). Synovial tissues from osteoarthritic patients and young patients with mild joint deformity were used as controls (n = 6). Tissue samples were fixed in 4% PFA, decalcified with EDTA, and embedded in paraffin. Sections (4 microm) were stained with hematoxylin/eosin and for the osteoclastic marker enzyme tartrate resistant acid phosphatase. Monocytes/macrophages were characterized with a monoclonal antibody against CD68 and mRNAs encoding MMP-13 and alpha(1) collagen I (COL1A1) were detected by in situ hybridization. Cells expressing transcripts encoding MMP-13 were found in 70% of the interface tissues. These cells colocalized with a cell population expressing COL1A1 mRNA, and were fibroblastic in appearance. MMP-13 expressing cells were found in the close vicinity of osteoclasts and multinuclear giant cells. No signals for transcripts encoding MMP-13 were detected in multinuclear giant cells or in osteoclasts. Control tissues were negative for transcripts encoding MMP-13 mRNA. Fibroblasts of the interface from aseptically loosened endoprostheses selectively express MMP-13. By the expression and the release of MMP-13, these fibroblastic cells may contribute to the local degradation of the extracellular matrix and to bone resorption.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Klinikum Grosshadern, Ludwig-Maximilians-University Munich, Marchioninistr. 15, 81377 Munich, Germany. susanne.wagner@med.uni-muenchen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17853491

Citation

Wagner, Susanne, et al. "Interface Membrane Fibroblasts Around Aseptically Loosened Endoprostheses Express MMP-13." Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, vol. 26, no. 2, 2008, pp. 143-52.
Wagner S, Gollwitzer H, Wernicke D, et al. Interface membrane fibroblasts around aseptically loosened endoprostheses express MMP-13. J Orthop Res. 2008;26(2):143-52.
Wagner, S., Gollwitzer, H., Wernicke, D., Langer, R., Siebenrock, K. A., & Hofstetter, W. (2008). Interface membrane fibroblasts around aseptically loosened endoprostheses express MMP-13. Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 26(2), 143-52.
Wagner S, et al. Interface Membrane Fibroblasts Around Aseptically Loosened Endoprostheses Express MMP-13. J Orthop Res. 2008;26(2):143-52. PubMed PMID: 17853491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interface membrane fibroblasts around aseptically loosened endoprostheses express MMP-13. AU - Wagner,Susanne, AU - Gollwitzer,Hans, AU - Wernicke,Dirk, AU - Langer,Rupert, AU - Siebenrock,Klaus A, AU - Hofstetter,Willy, PY - 2007/9/14/pubmed PY - 2008/2/6/medline PY - 2007/9/14/entrez SP - 143 EP - 52 JF - Journal of orthopaedic research : official publication of the Orthopaedic Research Society JO - J Orthop Res VL - 26 IS - 2 N2 - The objective of this article was to assess whether matrix metalloproteinase-13 (MMP-13) is produced by cells of the peri-implant interface tissues and to further characterize these cells. Tissue specimens were collected from the bone-prosthesis interface at the time of revision surgery of clinically loosened hip and knee arthroplasties (n = 27). Synovial tissues from osteoarthritic patients and young patients with mild joint deformity were used as controls (n = 6). Tissue samples were fixed in 4% PFA, decalcified with EDTA, and embedded in paraffin. Sections (4 microm) were stained with hematoxylin/eosin and for the osteoclastic marker enzyme tartrate resistant acid phosphatase. Monocytes/macrophages were characterized with a monoclonal antibody against CD68 and mRNAs encoding MMP-13 and alpha(1) collagen I (COL1A1) were detected by in situ hybridization. Cells expressing transcripts encoding MMP-13 were found in 70% of the interface tissues. These cells colocalized with a cell population expressing COL1A1 mRNA, and were fibroblastic in appearance. MMP-13 expressing cells were found in the close vicinity of osteoclasts and multinuclear giant cells. No signals for transcripts encoding MMP-13 were detected in multinuclear giant cells or in osteoclasts. Control tissues were negative for transcripts encoding MMP-13 mRNA. Fibroblasts of the interface from aseptically loosened endoprostheses selectively express MMP-13. By the expression and the release of MMP-13, these fibroblastic cells may contribute to the local degradation of the extracellular matrix and to bone resorption. SN - 1554-527X UR - https://www.unboundmedicine.com/medline/citation/17853491/Interface_membrane_fibroblasts_around_aseptically_loosened_endoprostheses_express_MMP_13_ L2 - https://doi.org/10.1002/jor.20494 DB - PRIME DP - Unbound Medicine ER -